Results 31 to 40 of about 5,030,666 (315)

Single-molecule detection on a portable 3D-printed microscope

open access: yesNature Communications, 2019
Single-molecule in vitro assays require dedicated confocal microscopes equipped with fluorescence correlation spectroscopy (FCS) modules. Here the authors present a compact, cheap and open-source 3D-printed confocal microscope for single photon counting ...
James W. P. Brown   +7 more
doaj   +1 more source

Single-Molecule Reaction-Diffusion

open access: yesBiophysical Journal, 2023
Abstract We propose to capture reaction-diffusion on a molecule-by-molecule basis from the fastest acquirable timescale, namely individual photon arrivals. We illustrate our method on intrinsically disordered human proteins, the linker histone H1.0 as well as its chaperone prothymosin α
Lance W.Q. Xu   +3 more
openaire   +3 more sources

Protocol to generate fast-dissociating recombinant antibody fragments for multiplexed super-resolution microscopy

open access: yesSTAR Protocols, 2023
Summary: Multiplexed high-density label super-resolution microscopy image reconstruction by integrating exchangeable single-molecule localization (IRIS) enables elucidating fine structures and molecular distribution in cells and tissues.
Qianli Zhang   +2 more
doaj   +1 more source

A single-molecule diode [PDF]

open access: yesProceedings of the National Academy of Sciences, 2005
We have designed and synthesized a molecular rod that consists of two weakly coupled electronic π -systems with mutually shifted energy levels. The asymmetry thus implied manifests itself in a current–voltage characteristic with pronounced dependence on the sign of the bias voltage, which makes the molecule a prototype for a molecular diode.
Elbing, M.   +9 more
openaire   +3 more sources

A Memristive Element Based on an Electrically Controlled Single‐Molecule Reaction [PDF]

open access: yes, 2020
A single-molecule memory element is electrically-controlled using two distinct reaction mechanisms as reported by K. Moth-Poulsen, J. Hihath, and co-workers (DOI:10.1002/anie.202002300).
Haipeng B. Li   +14 more
core   +1 more source

Single molecules in fluorescence fluctuation spectroscopy: Effective volume and photon counting histogram

open access: yes, 2022
Paper 686204This paper presents a stochastic theory for the interpretation of photon counting histograms in fluorescence fluctuation spectroscopy (FFS). New concepts of an effective volume and a single molecule probability distribution are introduced to ...
Greiner, B., Ackermann, J., Mathis, H.
core   +1 more source

Decoding the Structural Dynamics and Conformational Alternations of DNA Secondary Structures by Single-Molecule FRET Microspectroscopy

open access: yesFrontiers in Molecular Biosciences, 2021
In addition to the canonical double helix form, DNA is known to be extrapolated into several other secondary structural patterns involving themselves in inter- and intramolecular type hydrogen bonding. The secondary structures of nucleic acids go through
Debolina Bandyopadhyay   +3 more
doaj   +1 more source

Live cell imaging of single RNA molecules with fluorogenic Mango II arrays

open access: yesNature Communications, 2020
Fluorogenic RNA aptamers have been used for RNA imaging, but folding and fluorescence stability often limited their use in high resolution applications.
Adam D. Cawte   +2 more
doaj   +1 more source

Sphingomyelin metabolism underlies Ras excitability for efficient cell migration and chemotaxis

open access: yesCell Structure and Function, 2023
In eukaryotic motile cells, the active Ras (Ras-GTP)-enriched domain is generated in an asymmetric manner on the cell membrane through the excitable dynamics of an intracellular signaling network.
Da Young Shin   +4 more
doaj   +1 more source

Nanotechnology and Single Molecules

open access: yesChemPhysChem, 2004
For many years now, scientific and technological research has witnessed a dramatic increase of interest in studies of single molecules. A large number of ongoing projects are dealing with examinations of individual particles, and many of them have been funded by the Volkswagen Stiftung.
openaire   +3 more sources

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