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Characterizing 3D RNA structure by single molecule FRET
Methods, 2016The importance of elucidating the three dimensional structures of RNA molecules is becoming increasingly clear. However, traditional protein structural techniques such as NMR and X-ray crystallography have several important drawbacks when probing long RNA molecules.
James D, Stephenson +3 more
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Multiplexed Nucleic Acid Sensing with Single-Molecule FRET
ACS Sensors, 2019Multiplex detection of biomolecules is important in bionanotechnology and clinical diagnostics. Multiplexing using engineered solutions such as microarrays, synthetic nanopores, and DNA barcodes is promising, but they require sophisticated design/engineering and typically yield semiquantitative information. Single-molecule fluorescence resonance energy
Anisa Kaur, Kumar Sapkota, Soma Dhakal
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Macromolecular Models by Single Molecule FRET
2012Single molecule fluorescence energy transfer (FRET) experiments enable investigations of macromolecular conformation and folding by the introduction of fluorescent dyes at specific sites in the macromolecule. Multiple such experiments can be performed with different labeling site combinations in order to map complex conformational changes or ...
Axel T. Brunger +5 more
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Confocal Single-Molecule FRET for Protein Conformational Dynamics
2013Single-molecule Fӧrster-type resonance energy transfer (smFRET) is a unique technique capable of following conformational motions of individual protein molecules. The direct observation of individual proteins provides rich information that would be washed away in ensemble measurements, hence opening up new avenues for establishing the protein structure-
Yan-Wen, Tan +3 more
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Labeling proteins for single-molecule FRET.
Cold Spring Harbor protocols, 2013Single-molecule (sm) fluorescence detection is a powerful method for studying biological events without time and population averaging. Förster (fluorescence) resonance energy transfer (FRET) is a spectroscopic technique for measuring distances in the 30-80 Å range in which excitation energy of a donor molecule is transferred to an acceptor via ...
Chirlmin, Joo, Taekjip, Ha
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iSMS: single-molecule FRET microscopy software
Nature Methods, 2015Preus, Soren +4 more
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Förster resonance energy transfer (FRET)-based small-molecule sensors and imaging agents
Chemical Society Reviews, 2020Luling Wu, Chusen Huang, Ben P Emery
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Functionalized upconversion nanoparticles: New strategy towards FRET-based luminescence bio-sensing
Coordination Chemistry Reviews, 2021Anees A Ansari, Guanying Chen
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Self-assembled nanoscale biosensors based on quantum dot FRET donors
Nature Materials, 2003Igor L Medintz +2 more
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