Results 261 to 270 of about 62,512 (285)
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Characterizing 3D RNA structure by single molecule FRET

Methods, 2016
The importance of elucidating the three dimensional structures of RNA molecules is becoming increasingly clear. However, traditional protein structural techniques such as NMR and X-ray crystallography have several important drawbacks when probing long RNA molecules.
James D, Stephenson   +3 more
openaire   +2 more sources

Multiplexed Nucleic Acid Sensing with Single-Molecule FRET

ACS Sensors, 2019
Multiplex detection of biomolecules is important in bionanotechnology and clinical diagnostics. Multiplexing using engineered solutions such as microarrays, synthetic nanopores, and DNA barcodes is promising, but they require sophisticated design/engineering and typically yield semiquantitative information. Single-molecule fluorescence resonance energy
Anisa Kaur, Kumar Sapkota, Soma Dhakal
openaire   +2 more sources

Macromolecular Models by Single Molecule FRET

2012
Single molecule fluorescence energy transfer (FRET) experiments enable investigations of macromolecular conformation and folding by the introduction of fluorescent dyes at specific sites in the macromolecule. Multiple such experiments can be performed with different labeling site combinations in order to map complex conformational changes or ...
Axel T. Brunger   +5 more
openaire   +1 more source

Confocal Single-Molecule FRET for Protein Conformational Dynamics

2013
Single-molecule Fӧrster-type resonance energy transfer (smFRET) is a unique technique capable of following conformational motions of individual protein molecules. The direct observation of individual proteins provides rich information that would be washed away in ensemble measurements, hence opening up new avenues for establishing the protein structure-
Yan-Wen, Tan   +3 more
openaire   +2 more sources

Labeling proteins for single-molecule FRET.

Cold Spring Harbor protocols, 2013
Single-molecule (sm) fluorescence detection is a powerful method for studying biological events without time and population averaging. Förster (fluorescence) resonance energy transfer (FRET) is a spectroscopic technique for measuring distances in the 30-80 Å range in which excitation energy of a donor molecule is transferred to an acceptor via ...
Chirlmin, Joo, Taekjip, Ha
openaire   +1 more source

iSMS: single-molecule FRET microscopy software

Nature Methods, 2015
Preus, Soren   +4 more
openaire   +3 more sources

Förster resonance energy transfer (FRET)-based small-molecule sensors and imaging agents

Chemical Society Reviews, 2020
Luling Wu, Chusen Huang, Ben P Emery
exaly  

Functionalized upconversion nanoparticles: New strategy towards FRET-based luminescence bio-sensing

Coordination Chemistry Reviews, 2021
Anees A Ansari, Guanying Chen
exaly  

Self-assembled nanoscale biosensors based on quantum dot FRET donors

Nature Materials, 2003
Igor L Medintz   +2 more
exaly  

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