Results 251 to 260 of about 5,805,397 (279)

Single-Molecule FRET with Diffusion and Conformational Dynamics

The Journal of Physical Chemistry B, 2007
Under relatively mild conditions, we show how one can extract information about conformational dynamics from Förster resonance energy transfer (FRET) experiments on diffusing molecules without modeling diffusion. Starting from a rigorous theory that does treat diffusion, we first examine when the single-molecule FRET efficiency distribution can be ...
Irina V, Gopich, Attila, Szabo
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Distribution Analysis for Single Molecule FRET Measurement

The Journal of Physical Chemistry B, 2008
A new numerical analysis method for experimental single-pair fluorescence resonance energy transfer (sp-FRET) data is proposed. In this method, every single data point was plotted in a style of a cumulative distribution function and dedicated to curve-fitting analysis, so that the analysis does not depend on bin size.
Kenji, Okamoto, Masahide, Terazima
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Single-Molecule FRET-Based Dynamic DNA Sensor

ACS Sensors, 2021
Selective and sensitive detection of nucleic acid biomarkers is of great significance in early-stage diagnosis and targeted therapy. Therefore, the development of diagnostic methods capable of detecting diseases at the molecular level in biological fluids is vital to the emerging revolution in the early diagnosis of diseases. However, the vast majority
Anoja Megalathan, Kalani M. Wijesinghe, Soma Dhakal   +2 more
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iMAX FRET (Information Maximized FRET) for Multipoint Single-Molecule Structural Analysis [PDF]

Nano Letters
Understanding the structure of biomolecules is vital for deciphering their roles in biological systems. Single-molecule techniques have emerged as alternatives to conventional ensemble structure analysis methods for uncovering new biology in molecular ...
Mark Howarth, Sung Hyun Kim, Carlos de Lannoy   +2 more
exaly   +4 more sources

RNA Intramolecular Dynamics by Single‐Molecule FRET

Current Protocols in Nucleic Acid Chemistry, 2008
AbstractInvestigation of single RNA molecules using fluorescence resonance energy transfer (FRET) is a powerful approach to investigate dynamic and thermodynamic aspects of the folding process of a given RNA. Its application requires interdisciplinary work from the fields of chemistry, biochemistry, and physics.
Hengesbach, Martin, Kobitski, Andrei, Voigts-Hoffmann, Felix, Frauer, Carina, Nienhaus, G. Ulrich, Helm, Mark   +5 more
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Single-molecule FRET studies of ion channels

Progress in Biophysics and Molecular Biology, 2017
Different types of fluorescence spectroscopy approaches have over the last two decades become important techniques in studies of ion channel structure and dynamics. Many fluorescence methods have been used to examine a huge variety of ion channels. Any fluorescence study of ion channels requires the presence of fluorophores, which may be intrinsic to ...
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Single-molecule FRET studies of Cas9 endonuclease

2019
Since its discovery, the CRISPR-Cas9 system has been in the center of attention for its promising applications in genome editing. However, in order to apply this system successfully in genetic engineering, all aspects of its molecular mechanism have to be well understood. One of the best ways to investigate the intricacies of the molecular mechanism is
Viktorija, Globyte, Chirlmin, Joo
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Single-Molecule FRET-Based Multiplexed Detection

Single-molecule multiplexed detection is a high-promise toolkit for the expanding field of biosensing and molecular diagnostics. Among many single-molecule techniques available today for biomarker sensing including fluorescence, force, electrochemical, spectroscopic, barcoding, and other techniques, fluorescence-based approaches are arguably the most ...
Srishty, Sethi, Kalani M, Wijesinghe, Soma, Dhakal   +2 more
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Characterizing 3D RNA structure by single molecule FRET

Methods, 2016
The importance of elucidating the three dimensional structures of RNA molecules is becoming increasingly clear. However, traditional protein structural techniques such as NMR and X-ray crystallography have several important drawbacks when probing long RNA molecules.
James D, Stephenson, Julia C, Kenyon, Martyn F, Symmons, Andrew M L, Lever   +3 more
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Quantitative structural information from single-molecule FRET

Faraday Discussions, 2015
Single-molecule studies can be used to study biological processes directly and in real-time. In particular, the fluorescence energy transfer between reporter dye molecules attached to specific sites on macromolecular complexes can be used to infer distance information. When several measurements are combined, the information can be used to determine the
M, Beckers, F, Drechsler, T, Eilert, J, Nagy, J, Michaelis   +4 more
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