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Manipulating Protein Conformations by Single-Molecule AFM-FRET Nanoscopy [PDF]
ACS Nano, 2012 Combining atomic force microscopy and fluorescence resonance energy transfer spectroscopy (AFM-FRET), we have developed a single-molecule AFM-FRET nanoscopy approach capable of effectively pinpointing and mechanically manipulating a targeted dye-labeled ...
Maolin Lu, H Peter Lu, Yufan He
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2023
Fluorescence resonance energy transfer (FRET) is a photophysical phenomenon that has been repurposed as a biophysical tool to measure nanometer distances. With FRET by DNA eXchange, or FRET X, many points of interest (POIs) in a single object can be probed, overcoming a major limitation of conventional single-molecule FRET.
Filius, M. (author) +2 more
openaire +3 more sources
Fluorescence resonance energy transfer (FRET) is a photophysical phenomenon that has been repurposed as a biophysical tool to measure nanometer distances. With FRET by DNA eXchange, or FRET X, many points of interest (POIs) in a single object can be probed, overcoming a major limitation of conventional single-molecule FRET.
Filius, M. (author) +2 more
openaire +3 more sources
Correlative Single-Molecule FRET and DNA-PAINT Imaging
Nano Letters, 2018 DNA-PAINT is an optical super-resolution microscopy method that can visualize nanoscale protein arrangements and provide spectrally unlimited multiplexing capabilities.
Sebastian Malkusch +2 more
exaly +3 more sources
Single-Molecule FRET of Intrinsically Disordered Proteins
Annual Review of Physical Chemistry, 2020Intrinsically disordered proteins (IDPs) are now widely recognized as playing critical roles in a broad range of cellular functions as well as being implicated in diverse diseases. Their lack of stable secondary structure and tertiary interactions, coupled with their sensitivity to measurement conditions, stymies many traditional structural biology ...
Elizabeth Rhoades, Lauren Ann Metskas
exaly +4 more sources
Theory and Analysis of Single-Molecule FRET Experiments
2021Inter-dye distances and conformational dynamics can be studied using single-molecule FRET measurements. We consider two approaches to analyze sequences of photons with recorded photon colors and arrival times. The first approach is based on FRET efficiency histograms obtained from binned photon sequences.
Irina V, Gopich, Hoi Sung, Chung
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MASH-FRET: A Simplified Approach for Single-Molecule Multiplexing Using FRET
Analytical Chemistry, 2021Multiplexed detection has been a big motivation in biomarker analysis as it not only saves cost and labor but also improves the reliability of diagnosis. Among the many approaches for multiplexed detection, fluorescence resonance energy transfer (FRET)-based multiplexing is gaining popularity particularly due to its low background and quantitative ...
Anisa Kaur, Mischa Ellison, Soma Dhakal
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Preparing Sample Chambers for Single-Molecule FRET
Cold Spring Harbor Protocols, 2012Single-molecule (sm) fluorescence detection is a powerful method for studying biological events without time and population averaging. Förster (fluorescence) resonance energy transfer (FRET) is a spectroscopic technique in which the efficiency of energy transfer from donor to acceptor molecules is used to determine distances between molecules in the 30–
Chirlmin, Joo, Taekjip, Ha
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Single Molecules, 2001
Single molecule studies have revolutionized our understanding of how biomolecules work. The ability to watch one molecule at a time reveals not only the average properties detected in ensemble measurements, but can yield the entire distribution of relevant properties, including subpopulations and rare events.
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Single molecule studies have revolutionized our understanding of how biomolecules work. The ability to watch one molecule at a time reveals not only the average properties detected in ensemble measurements, but can yield the entire distribution of relevant properties, including subpopulations and rare events.
openaire +1 more source
Single-Molecule FRET with Diffusion and Conformational Dynamics
The Journal of Physical Chemistry B, 2007Under relatively mild conditions, we show how one can extract information about conformational dynamics from Förster resonance energy transfer (FRET) experiments on diffusing molecules without modeling diffusion. Starting from a rigorous theory that does treat diffusion, we first examine when the single-molecule FRET efficiency distribution can be ...
Irina V, Gopich, Attila, Szabo
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Distribution Analysis for Single Molecule FRET Measurement
The Journal of Physical Chemistry B, 2008A new numerical analysis method for experimental single-pair fluorescence resonance energy transfer (sp-FRET) data is proposed. In this method, every single data point was plotted in a style of a cumulative distribution function and dedicated to curve-fitting analysis, so that the analysis does not depend on bin size.
Kenji, Okamoto, Masahide, Terazima
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