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Single-Molecule FRET Analysis of Helicase Functions
2009In recent years, advancements in single-biomolecule probing techniques have provided critical information on and greater insight into the nature of biomolecules. Of significance is the application of single-molecule fluorescence resonance energy transfer (smFRET) to probe isolated events and changes at the nanometer scales.
Eli, Rothenberg, Taekjip, Ha
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Single-molecule FRET studies of Cas9 endonuclease
2019Since its discovery, the CRISPR-Cas9 system has been in the center of attention for its promising applications in genome editing. However, in order to apply this system successfully in genetic engineering, all aspects of its molecular mechanism have to be well understood. One of the best ways to investigate the intricacies of the molecular mechanism is
Viktorija, Globyte, Chirlmin, Joo
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Single-Molecule FRET-Based Dynamic DNA Sensor
ACS Sensors, 2021Selective and sensitive detection of nucleic acid biomarkers is of great significance in early-stage diagnosis and targeted therapy. Therefore, the development of diagnostic methods capable of detecting diseases at the molecular level in biological fluids is vital to the emerging revolution in the early diagnosis of diseases. However, the vast majority
Anoja Megalathan +2 more
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RNA Intramolecular Dynamics by Single‐Molecule FRET
Current Protocols in Nucleic Acid Chemistry, 2008AbstractInvestigation of single RNA molecules using fluorescence resonance energy transfer (FRET) is a powerful approach to investigate dynamic and thermodynamic aspects of the folding process of a given RNA. Its application requires interdisciplinary work from the fields of chemistry, biochemistry, and physics.
Hengesbach, Martin +5 more
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Single-molecule FRET studies of ion channels
Progress in Biophysics and Molecular Biology, 2017Different types of fluorescence spectroscopy approaches have over the last two decades become important techniques in studies of ion channel structure and dynamics. Many fluorescence methods have been used to examine a huge variety of ion channels. Any fluorescence study of ion channels requires the presence of fluorophores, which may be intrinsic to ...
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Single-Molecule FRET Analysis of Replicative Helicases
2018Over the recent years single-molecule fluorescence resonance energy transfer (smFRET) technique has proven to be one of the most powerful tools for revealing mechanistic insights into helicase activities. Here we describe details of single-molecule FRET assays for probing DNA unwinding activities as well as functional dynamics by replicative helicases ...
Seung-Jae, Lee +2 more
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Single-Molecule FRET-Based Multiplexed Detection
Single-molecule multiplexed detection is a high-promise toolkit for the expanding field of biosensing and molecular diagnostics. Among many single-molecule techniques available today for biomarker sensing including fluorescence, force, electrochemical, spectroscopic, barcoding, and other techniques, fluorescence-based approaches are arguably the most ...Srishty, Sethi +2 more
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Single molecule FRET methodology for investigating glutamate receptors
2021Single molecule Förster Resonance Energy Transfer (smFRET) allows us to measure variation in distances between donor and acceptor fluorophores attached to a protein, providing the conformational landscape of the protein with respect to this specific distance. smFRET can be performed on freely diffusing molecules or on tethered molecules.
Nabina, Paudyal +2 more
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Quantitative structural information from single-molecule FRET
Faraday Discussions, 2015Single-molecule studies can be used to study biological processes directly and in real-time. In particular, the fluorescence energy transfer between reporter dye molecules attached to specific sites on macromolecular complexes can be used to infer distance information. When several measurements are combined, the information can be used to determine the
M, Beckers +4 more
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Multiplexed Nucleic Acid Sensing with Single-Molecule FRET
ACS Sensors, 2019Multiplex detection of biomolecules is important in bionanotechnology and clinical diagnostics. Multiplexing using engineered solutions such as microarrays, synthetic nanopores, and DNA barcodes is promising, but they require sophisticated design/engineering and typically yield semiquantitative information. Single-molecule fluorescence resonance energy
Anisa Kaur, Kumar Sapkota, Soma Dhakal
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