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2023
Fluorescence resonance energy transfer (FRET) is a photophysical phenomenon that has been repurposed as a biophysical tool to measure nanometer distances. With FRET by DNA eXchange, or FRET X, many points of interest (POIs) in a single object can be probed, overcoming a major limitation of conventional single-molecule FRET.
Filius, M. (author) +2 more
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Fluorescence resonance energy transfer (FRET) is a photophysical phenomenon that has been repurposed as a biophysical tool to measure nanometer distances. With FRET by DNA eXchange, or FRET X, many points of interest (POIs) in a single object can be probed, overcoming a major limitation of conventional single-molecule FRET.
Filius, M. (author) +2 more
openaire +3 more sources
Single-Molecule FRET to Measure Conformational Dynamics of DNA Mismatch Repair Proteins [PDF]
Methods in Enzymology, 2016 Single-molecule FRET measurements have a unique sensitivity to protein conformational dynamics. The FRET signals can either be interpreted quantitatively to provide estimates of absolute distance in a molecule configuration or can be qualitatively ...
Sharonda Leblanc, Ruoyi Qiu
exaly +2 more sources
Correlative Single-Molecule FRET and DNA-PAINT Imaging
Nano Letters, 2018 DNA-PAINT is an optical super-resolution microscopy method that can visualize nanoscale protein arrangements and provide spectrally unlimited multiplexing capabilities.
Sebastian Malkusch +2 more
exaly +3 more sources
Single-Molecule FRET of Intrinsically Disordered Proteins
Annual Review of Physical Chemistry, 2020Intrinsically disordered proteins (IDPs) are now widely recognized as playing critical roles in a broad range of cellular functions as well as being implicated in diverse diseases. Their lack of stable secondary structure and tertiary interactions, coupled with their sensitivity to measurement conditions, stymies many traditional structural biology ...
Metskas, Lauren Ann, Rhoades, Elizabeth
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Theory and Analysis of Single-Molecule FRET Experiments
2021Inter-dye distances and conformational dynamics can be studied using single-molecule FRET measurements. We consider two approaches to analyze sequences of photons with recorded photon colors and arrival times. The first approach is based on FRET efficiency histograms obtained from binned photon sequences.
Irina V, Gopich, Hoi Sung, Chung
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MASH-FRET: A Simplified Approach for Single-Molecule Multiplexing Using FRET
Analytical Chemistry, 2021Multiplexed detection has been a big motivation in biomarker analysis as it not only saves cost and labor but also improves the reliability of diagnosis. Among the many approaches for multiplexed detection, fluorescence resonance energy transfer (FRET)-based multiplexing is gaining popularity particularly due to its low background and quantitative ...
Anisa Kaur, Mischa Ellison, Soma Dhakal
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Preparing Sample Chambers for Single-Molecule FRET
Cold Spring Harbor Protocols, 2012Single-molecule (sm) fluorescence detection is a powerful method for studying biological events without time and population averaging. Förster (fluorescence) resonance energy transfer (FRET) is a spectroscopic technique in which the efficiency of energy transfer from donor to acceptor molecules is used to determine distances between molecules in the 30–
Chirlmin, Joo, Taekjip, Ha
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Single Molecules, 2001
Single molecule studies have revolutionized our understanding of how biomolecules work. The ability to watch one molecule at a time reveals not only the average properties detected in ensemble measurements, but can yield the entire distribution of relevant properties, including subpopulations and rare events.
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Single molecule studies have revolutionized our understanding of how biomolecules work. The ability to watch one molecule at a time reveals not only the average properties detected in ensemble measurements, but can yield the entire distribution of relevant properties, including subpopulations and rare events.
openaire +1 more source
Single-Molecule FRET Analysis of Helicase Functions
2009In recent years, advancements in single-biomolecule probing techniques have provided critical information on and greater insight into the nature of biomolecules. Of significance is the application of single-molecule fluorescence resonance energy transfer (smFRET) to probe isolated events and changes at the nanometer scales.
Eli, Rothenberg, Taekjip, Ha
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Single-Molecule FRET Analysis of Replicative Helicases
2018Over the recent years single-molecule fluorescence resonance energy transfer (smFRET) technique has proven to be one of the most powerful tools for revealing mechanistic insights into helicase activities. Here we describe details of single-molecule FRET assays for probing DNA unwinding activities as well as functional dynamics by replicative helicases ...
Seung-Jae, Lee +2 more
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