Results 241 to 250 of about 258,562 (263)

Single-Cell RNA Sequencing Outperforms Single-Nucleus RNA Sequencing in Analyzing Pancreatic Cell Diversity and Gene Expression in Goats. [PDF]

Int J Mol Sci
Cheng J, Zhang T, Cheng Y, Gebeyew K, Tan Z, He Z.   +5 more
europepmc   +1 more source

Single-Nucleus RNA Sequencing Reveals the Transcriptome Profiling of Ovarian Cells in Adolescent Cyprinus carpio. [PDF]

Animals (Basel)
Hou M, Zhang J, Wang Q, Zhao R, Cao Y, Chen Y, Wang K, Ding N, Qi Y, Sun X, Zhang Y, Li J.   +11 more
europepmc   +1 more source

Single-nucleus RNA sequencing reveals Cntnap2⁺ astrocytes triggering a hypertensive effect in the rostral ventrolateral medulla by disrupting glutamate uptake. [PDF]

J Adv Res
Zhang S, Tong L, Ren Z, Dai T, Wang L, Zhu D, Liu H, Zhu L, Huang D, Du D.   +9 more
europepmc   +1 more source

Single-Nucleus RNA-Sequencing Identifies a Differential Profibrotic Response in Parietal Epithelial Cells in Primary Versus Maladaptive Focal Segmental Glomerulosclerosis. [PDF]

Kidney Int Rep
Deleersnijder D, Venken T, Schepers R, Van Brussel T, Meijers BK, Sprangers B, Lambrechts D, Van Craenenbroeck AH.   +7 more
europepmc   +1 more source

Single-Nucleus RNA Sequencing Reveals Muscle-Region-Specific Differences in Fibro-Adipogenic Progenitors Driving Intramuscular Fat Accumulation. [PDF]

Metabolites
Ueda S, Kitamura C, Tateoka Y, Kanai A, Suzuki Y, Fukuda I, Shirai Y.   +6 more
europepmc   +1 more source

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Multiplexed Single‐Nucleus RNA Sequencing Using Lipid‐Oligo Barcodes

Current Protocols, 2022
AbstractThis protocol describes a robust pipeline for simultaneously analyzing multiple samples by single‐nucleus (sn)RNA‐seq. cDNA obtained from each single sample are labeled with the same lipid‐coupled oligonucleotide barcode (10X Genomics). Nuclei from as many as 12 individual samples can be pooled together and simultaneously processed for cDNA ...
Qi, Zhang, Seong Won, Kim, Joshua M, Gorham, Daniel M, DeLaughter, Tarsha, Ward, Christine E, Seidman, Jonathan G, Seidman   +6 more
openaire   +2 more sources

Single nucleus RNA-sequencing: how it's done, applications and limitations

Emerging Topics in Life Sciences, 2021
Single nuclei RNA-sequencing (sNuc-Seq) is a methodology which uses isolated nuclei instead of whole cells to profile gene expression. By using droplet microfluidic technologies, users are able to profile thousands of single transcriptomes at high throughput from their chosen tissue.
Juliane Fischer, Thomas Ayers
openaire   +2 more sources

Plant Nuclei Isolation for Single-Nucleus RNA Sequencing

2023
Transcriptome profiling has been significantly hampered by the heterogeneity among individual cells within a tissue or an organ. Recent advances in single cell transcriptome profiling have significantly advanced our understanding of the transcriptome. However, plant single-cell RNA sequencing (scRNA-seq) relies on the isolation of protoplasts, which is
Xu, Xin, Fei, Du, Yuling, Jiao
openaire   +2 more sources

Isolating Nuclei From Frozen Human Heart Tissue for Single‐Nucleus RNA Sequencing

Current Protocols, 2022
AbstractHeart disease is the leading cause of global morbidity and mortality. This is in part because, despite an abundance of animal and in vitro models, it has been a challenge to date to study human heart tissue with sufficient depth and resolution to develop disease‐modifying therapies for common cardiac conditions.
Safabakhsh, S., Sar, F., Martelotto, L., Haegert, A., Singhera, G., Hanson, P., Parker, J., Collins, C., Rohani, L., Laksman, Z.   +9 more
openaire   +3 more sources