Results 251 to 260 of about 258,562 (263)

Large-Scale Single-Nucleus RNA Sequencing Compatible with Complex Archived Samples

2022
Transcriptome profiling at single-cell resolution allows us to identify and assess functional cell types and cellular states, including those within degenerating ocular tissues in retinitis pigmentosa. The technology is particularly valuable when studying tissues with high cellular heterogeneity, or when specific cell types are of interest.
Chao-Yu, Liu, Hsu-Hsin, Chen
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Updated skin transcriptomic atlas depicted by reciprocal contribution of single-nucleus RNA sequencing and single-cell RNA sequencing

Journal of Dermatological Science, 2023
Single-cell RNA sequencing (scRNA-seq) has advanced our understanding of skin biology, but its utility is restricted by the requirement of fresh samples, inadequate dissociation-induced cell loss or death, and activation during tissue digestion. Single-nucleus RNA sequencing (snRNA-seq) can use frozen, hard-to-dissociate materials, which might be a ...
Ronghui, Zhu, Xiaoyu, Pan, Shangshang, Wang, Zhuoqiong, Qiu, Chaoying, Gu, Xu, Yao, Wei, Li   +6 more
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Single Nucleus Total RNA Sequencing of Formalin‐Fixed Paraffin‐Embedded Gliomas

Small Methods
AbstractGliomas, the predominant form of brain cancer, comprise diverse malignant subtypes with limited curative therapies available. The insufficient understanding of their molecular diversity and evolutionary processes hinders the advancement of new treatments.
Ziye Xu, Lingchao Chen, Xin Lin, Yuexiao Lyu, Mofei Zhou, Haide Chen, Heng Zhang, Tianyu Zhang, Yu Chen, Yuanzhen Suo, Qian Liang, Zhiyong Qin, Yongcheng Wang   +12 more
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Abstract 2626: Leveraging FFPE single-nucleus RNA-sequencing for Osteosarcoma

Cancer Research
Abstract Osteosarcoma (OS), a heterogeneous class of bone sarcomas, can be subclassified based on predominant histological features. This molecular heterogeneity cannot fully be resolved with bulk sequencing. However, due to the rarity of several OS subtypes, including low-grade and small-cell, parosteal, periosteal, and high-grade ...
Danh Truong, Clement Agyemang, Davis Ingram, Alexander Lazar, Valerae O. Lewis, Joseph Ludwig   +5 more
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Single-nucleus RNA sequencing of diabetic mice

Mice were deeply anesthetized with isoflurane and hippocampus tissues were rapidly dissected for snRNA-seq.
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Single-nucleus RNA sequencing of Macaque hippocampus

Single-nucleus RNA sequencing of Macaque ...
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500 Single-Nucleus RNA Sequencing Reveals Mechanism for Multiple Recurrent Meningiomas

Neurosurgery
INTRODUCTION: Patients diagnosed with multiple recurrent meningiomas (MRMs) have worsened long-term neurological morbidity, compared to patients diagnosed with solitary meningiomas. However, the pathophysiology of these tumors is not clear.
Ihika Rampalli, Collin English, Akdes Harmanci, Tiemo Klisch, Akash J. Patel   +4 more
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Single‐Nucleus RNA Sequencing Unravels Early Mechanisms of Human Becker Muscular Dystrophy

Annals of Neurology
ObjectiveThe transcriptional heterogeneity at a single‐nucleus level in human Becker muscular dystrophy (BMD) dystrophic muscle has not been explored. Here, we aimed to understand the transcriptional heterogeneity associated with myonuclei, as well as other mononucleated cell types that underly BMD pathogenesis by performing single‐nucleus RNA ...
Zhihao Xie, Chang Liu, Chengyue Sun, Yilin Liu, Jieru Peng, Lingchao Meng, Jianwen Deng, Zhaoxia Wang, Chunxia Yang, Yun Yuan, Zhiying Xie   +10 more
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Single-nucleus RNA sequencing of rostral ventromedial medulla in mice with trigeminal neuralgia

International Journal of Neuroscience
Aim: To investigate the transcriptional changes and cell interactions following trigeminal neuralgia (TN) in different cell types in rostral ventromedial medulla (RVM).Methods: In this study, trigeminal neuropathic pain was induced in mice by ligating the left infraorbital nerve. Ten days after nerve ligation, we performed single-nucleus RNA sequencing
Xia, Zhang, Liuhanhui, Guo, Jingyan, Lyu, Xinrui, Li, Yucheng, Hao, Yanli, Zhang, Xiaofeng, Bai   +6 more
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PROTOCOL DEVELOPMENT FOR SINGLE-NUCLEUS RNA SEQUENCING OF HUMAN HEART TISSUE

Canadian Journal of Cardiology, 2022
S. Safabakhsh, F. Sar, L. Martelotto, A. Haegert, G. Singhera, P. Hanson, J. Parker, C. Collins, L. Rohani, Z. Laksman   +9 more
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