RNA Sequencing Reveals a Role of TonEBP Transcription Factor in Regulation of Pro-inflammatory Genes in Response to Hyperosmolarity in Healthy Nucleus Pulposus Cells: A HOMEOSTATIC RESPONSE? [PDF]
, 2016 Transcription factor tonicity-responsive enhancer-binding protein (TonEBP/NFAT5) is critical for osmo-adaptation and extracellular matrix homeostasis of nucleus pulposus (NP) cells in their hypertonic tissue niche.
Johnson, Zariel I. +2 more
core +2 more sources
miR-9 Acts as an OncomiR in Prostate Cancer through Multiple Pathways That Drive Tumour Progression and Metastasis [PDF]
, 2016 Identification of dysregulated microRNAs (miRNAs) in prostate cancer is critical not only for diagnosis, but also differentiation between the aggressive and indolent forms of the disease.
Budd, W. +6 more
core +8 more sources
Nature Communications, 2023 Formalin-fixed paraffin-embedded (FFPE) tissues constitute a vast and valuable patient material bank for clinical history and follow-up data. It is still challenging to achieve single cell/nucleus RNA (sc/snRNA) profile in FFPE tissues.
Ziye Xu +22 more
doaj +1 more source
Isolation of nuclei from mouse white adipose tissues for single-nucleus genomics
STAR Protocols, 2021 Summary: Lipid-filled adipocytes are incompatible with droplet-based single-cell methods, such as 10x Genomics-based technology, thus restricting droplet-based single-cell analyses of adipose tissues to the stromal vascular fraction.
Elvira Laila Van Hauwaert +6 more
doaj +1 more source
Genome-wide profiling of chromosome interactions in Plasmodium falciparum characterizes nuclear architecture and reconfigurations associated with antigenic variation. [PDF]
, 2013 Spatial relationships within the eukaryotic nucleus are essential for proper nuclear function. In Plasmodium falciparum, the repositioning of chromosomes has been implicated in the regulation of the expression of genes responsible for antigenic variation,
Berriman, Matthew +8 more
core +1 more source
Quantification and statistical modeling of droplet-based single-nucleus RNA-sequencing data
Biostatistics, 2023 Summary In complex tissues containing cells that are difficult to dissociate, single-nucleus RNA-sequencing (snRNA-seq) has become the preferred experimental technology over single-cell RNA-sequencing (scRNA-seq) to measure gene expression.
Albert Kuo +2 more
openaire +2 more sources
Cells, 2022 Recent advances in developmental biology have been made possible by using multi-omic studies at single cell resolution. However, progress in plants has been slowed, owing to the tremendous difficulty in protoplast isolation from most plant tissues and/or
Ming-Chao Yang +4 more
doaj +1 more source
Characterizing the composition of iPSC derived cells from bulk transcriptomics data with CellMap
Scientific Reports, 2022 Induced pluripotent stem cell (iPSC) derived cell types are increasingly employed as in vitro model systems for drug discovery. For these studies to be meaningful, it is important to understand the reproducibility of the iPSC-derived cultures and their ...
Zhengyu Ouyang +11 more
doaj +1 more source
On the extent and role of the small proteome in the parasitic eukaryote Trypanosoma brucei [PDF]
, 2014 Background: Although technical advances in genomics and proteomics research have yielded a better understanding of the coding capacity of a genome, one major challenge remaining is the identification of all expressed proteins, especially those less than ...
Butter, F. +5 more
core +2 more sources
CRISPR-Cas9 screens in human cells and primary neurons identify modifiers of C9ORF72 dipeptide-repeat-protein toxicity. [PDF]
, 2018 Hexanucleotide-repeat expansions in the C9ORF72 gene are the most common cause of amyotrophic lateral sclerosis and frontotemporal dementia (c9ALS/FTD).
A Berson +95 more
core +1 more source