Results 31 to 40 of about 261,642 (181)

Assessing Markovian and Delay Models for Single-Nucleus RNA Sequencing

Bulletin of Mathematical Biology, 2023
The serial nature of reactions involved in the RNA life-cycle motivates the incorporation of delays in models of transcriptional dynamics. The models couple a transcriptional process to a fairly general set of delayed monomolecular reactions with no feedback.
Gorin, Gennady, Yoshida, Shawn, Pachter, Lior   +2 more
openaire   +4 more sources

Single‐nucleus RNA sequencing reveals RUNX1 regulation of muscle hypertrophy through PI3K/AKT/mTOR pathway [PDF]

iMeta
Chenxu Wang, Junjie Ma, Yibin Wang, Rui Liu, Chenxi Zhang, Qingyuan Li, Lixin Zhang, Qihang Hou, Xiaojun Yang   +8 more
doaj   +2 more sources

Comparison of cell type distribution between single-cell and single-nucleus RNA sequencing: enrichment of adherent cell types in single-nucleus RNA sequencing

Experimental & Molecular Medicine, 2022
AbstractSingle-cell ribonucleic acid (RNA) sequencing (scRNA-seq) is an effective technique for estimating the cellular composition and transcriptional profiles of individual cells from fresh tissue. Single-nucleus RNA sequencing (snRNA-seq) is necessary to perform this type of analysis in frozen or difficult-to-dissociate tissues, which cannot be ...
Jin-Mi Oh, Minae An, Dae-Soon Son, Jinhyuk Choi, Yong Beom Cho, Chang Eun Yoo, Woong-Yang Park   +6 more
openaire   +2 more sources

Microglia Heterogeneity in Alzheimer’s Disease: Insights From Single-Cell Technologies

Frontiers in Synaptic Neuroscience, 2021
Microglia are resident immune cells in the central nervous system and play critical roles in brain immunity, development, and homeostasis. The pathology of Alzheimer’s disease (AD) triggers activation of microglia.
Hansen Wang
doaj   +1 more source

Simultaneous profiling of transcriptome and DNA methylome from a single cell. [PDF]

, 2016
BackgroundSingle-cell transcriptome and single-cell methylome technologies have become powerful tools to study RNA and DNA methylation profiles of single cells at a genome-wide scale. A major challenge has been to understand the direct correlation of DNA
An, Qin, Du, Guizhen, Fan, Guoping, Hu, Ganlu, Hu, Youjin, Huang, Kevin, Wang, Cun-Yu, Xue, Jinfeng, Xue, Zhigang, Zhu, Xianmin   +9 more
core   +3 more sources

Single‐nucleus RNA sequencing of plant tissues using a nanowell‐based system [PDF]

The Plant Journal, 2021
SUMMARYSingle‐cell genomics provides unprecedented potential for research on plant development and environmental responses. Here, we introduce a generic procedure for plant nucleus isolation combined with nanowell‐based library preparation. Our method enables the transcriptome analysis of thousands of individual plant nuclei.
Sunaga‐Franze, Daniele Y., Muino, Jose M., Braeuning, Caroline, Xu, Xiaocai, Zong, Minglei, Smaczniak, Cezary, Yan, Wenhao, Fischer, Cornelius, Vidal, Ramon, Kliem, Magdalena, Kaufmann, Kerstin, Sauer, Sascha   +11 more
openaire   +3 more sources

A Regulatory Circuitry Between Gria2, miR-409, and miR-495 Is Affected by ALS FUS Mutation in ESC-Derived Motor Neurons [PDF]

, 2018
Mutations in fused in sarcoma (FUS) cause amyotrophic lateral sclerosis (ALS). FUS is a multifunctional protein involved in the biogenesis and activity of several types of RNAs, and its role in the pathogenesis of ALS may involve both direct effects of
Biscarini, Silvia, Bozzoni, Irene, Caffarelli, Elisa, Capauto, Davide, Colantoni, Alessio, Laneve, Pietro, Lu, Lei, Morlando, Mariangela, Peruzzi, Giovanna, Santini, Tiziana, Shneider, Neil A.   +10 more
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The Nefarious Nexus of Noncoding RNAs in Cancer [PDF]

, 2018
The past decade has witnessed enormous progress, which has seen the noncoding RNAs (ncRNAs) turn from the so called dark matter RNA to critical functional molecules, influencing most physiological processes in development and disease contexts.
Anastasiadou, Eleni, Faggioni, Alberto, J Slack, Frank, Trivedi, Pankaj   +3 more
core   +1 more source

Non-Coding RNA Sequencing of Equine Endometrium During Maternal Recognition of Pregnancy. [PDF]

, 2019
Maternal recognition of pregnancy (MRP) in the mare is not well defined. In a non-pregnant mare, prostaglandin F2α (PGF) is released on day 14 post-ovulation (PO) to cause luteal regression, resulting in loss of progesterone production. Equine MRP occurs
Bouma, Gerrit J, Bruemmer, Jason E, Cameron, Ashley D, Canovas, Angela, Coleman, Stephen J, Hess, Ann M, Islas-Trejo, Alma D, Kalbfleisch, Ted, Klohonatz, Kristin M, Medrano, Juan F, Reed, Kailee J   +10 more
core   +1 more source

Systematic assessment of tissue dissociation and storage biases in single-cell and single-nucleus RNA-seq workflows

Genome Biology, 2020
Background Single-cell RNA sequencing has been widely adopted to estimate the cellular composition of heterogeneous tissues and obtain transcriptional profiles of individual cells. Multiple approaches for optimal sample dissociation and storage of single
Elena Denisenko, Belinda B. Guo, Matthew Jones, Rui Hou, Leanne de Kock, Timo Lassmann, Daniel Poppe, Olivier Clément, Rebecca K. Simmons, Ryan Lister, Alistair R. R. Forrest   +10 more
doaj   +1 more source