Results 21 to 30 of about 165,721 (244)

Optimized nucleus isolation protocol from frozen mouse tissues for single nucleus RNA sequencing application

Frontiers in Cell and Developmental Biology, 2023
The single cell RNA sequencing technique has been particularly used during the last years, allowing major discoveries. However, the widespread application of this analysis has showed limitations.
Marie-Albane Minati, Angeline Fages, Nicolas Dauguet, Nicolas Dauguet, Jingjing Zhu, Patrick Jacquemin   +5 more
doaj   +1 more source

Use of scREAD to explore and analyze single-cell and single-nucleus RNA-seq data for Alzheimer’s disease

STAR Protocols, 2021
Summary: Single-cell RNA-sequencing (scRNA-seq) and single-nucleus RNA-sequencing (snRNA-seq) studies have provided remarkable insights into understanding the molecular pathogenesis of Alzheimer's disease.
Cankun Wang, Yujia Xiang, Hongjun Fu, Qin Ma   +3 more
doaj   +1 more source

Comparison of cell type distribution between single-cell and single-nucleus RNA sequencing: enrichment of adherent cell types in single-nucleus RNA sequencing

Experimental & Molecular Medicine, 2022
AbstractSingle-cell ribonucleic acid (RNA) sequencing (scRNA-seq) is an effective technique for estimating the cellular composition and transcriptional profiles of individual cells from fresh tissue. Single-nucleus RNA sequencing (snRNA-seq) is necessary to perform this type of analysis in frozen or difficult-to-dissociate tissues, which cannot be ...
Jin-Mi Oh, Minae An, Dae-Soon Son, Jinhyuk Choi, Yong Beom Cho, Chang Eun Yoo, Woong-Yang Park   +6 more
openaire   +2 more sources

Microglia Heterogeneity in Alzheimer’s Disease: Insights From Single-Cell Technologies

Frontiers in Synaptic Neuroscience, 2021
Microglia are resident immune cells in the central nervous system and play critical roles in brain immunity, development, and homeostasis. The pathology of Alzheimer’s disease (AD) triggers activation of microglia.
Hansen Wang
doaj   +1 more source

Comparative analysis of nuclei isolation methods for brain single-nucleus RNA sequencing. [PDF]

Cell Rep Methods
SUMMARY Single-nucleus RNA sequencing (snRNA-seq) enables resolving cellular heterogeneity in complex tissues. snRNA-seq overcomes limitations of traditional single-cell RNA-seq by using nuclei instead of cells, allowing to utilize frozen tissues and difficult-to-isolate cell types.
Kersey HN, Acri DJ, Dabin LC, Hartigan KA, Mustaklem R, Park JH, Kim J.   +6 more
europepmc   +4 more sources

Understanding and evaluating ambiguity in single-cell and single-nucleus RNA-sequencing

, 2023
Recently, a new modification has been proposed by Hjörleifsson and Sullivan et al . to the model used to classify the splicing status of reads (as spliced (mature), unspliced (nascent), or ambiguous) in single-cell and single-nucleus RNA-seq data.
Dongze He, Charlotte Soneson, Rob Patro
openaire   +2 more sources

Quantification and statistical modeling of droplet-based single-nucleus RNA-sequencing data

Biostatistics, 2023
Summary In complex tissues containing cells that are difficult to dissociate, single-nucleus RNA-sequencing (snRNA-seq) has become the preferred experimental technology over single-cell RNA-sequencing (scRNA-seq) to measure gene expression.
Albert Kuo, Kasper D Hansen, Stephanie C Hicks   +2 more
openaire   +2 more sources

Single‐nucleus RNA sequencing of plant tissues using a nanowell‐based system [PDF]

The Plant Journal, 2021
SUMMARYSingle‐cell genomics provides unprecedented potential for research on plant development and environmental responses. Here, we introduce a generic procedure for plant nucleus isolation combined with nanowell‐based library preparation. Our method enables the transcriptome analysis of thousands of individual plant nuclei.
Sunaga‐Franze, Daniele Y., Muino, Jose M., Braeuning, Caroline, Xu, Xiaocai, Zong, Minglei, Smaczniak, Cezary, Yan, Wenhao, Fischer, Cornelius, Vidal, Ramon, Kliem, Magdalena, Kaufmann, Kerstin, Sauer, Sascha   +11 more
openaire   +2 more sources

Transcript-guided targeted cell enrichment for scalable single-nucleus RNA sequencing. [PDF]

Cell Genom
Large-scale single-cell atlases have revealed many aging- and disease-associated cell types, yet these populations are often underrepresented in heterogeneous tissues, limiting detailed molecular analyses. To address this, we developed EnrichSci-a scalable, microfluidics-free platform that combines hybridization chain reaction RNA fluorescence in situ ...
Liao A, Zhang Z, Sziraki A, Abdulraouf A, Rehman A, Xu Z, Lu Z, Jiang W, Arya A, Lee J, Maragkakis M, Zhou W, Cao J.   +12 more
europepmc   +3 more sources

Single-nucleus RNA sequencing illuminates a functional analog of lymphoid organ in crustacean. [PDF]

J Adv Res
The evolution of lymphoid organs is a complex topic in the animal kingdom. A presumptive invertebrate lymphoid organ (Oka) was previously reported in shrimp based on its morphological and histological observations. However, whether it truly functions as a lymphoid organ akin to those in vertebrates remains uncertain.This study aims to characterize the ...
Sun M, Li S, Liu Y, Li F.
europepmc   +3 more sources