Results 21 to 30 of about 261,642 (181)

Single Nucleus RNA Sequencing of Presumed Human Heart Failure Myocardium

Journal of Molecular and Cellular Cardiology Plus
Deborah Eaton, Kenneth Bedi, Ryan Pfeiffer, Nathan Tucker, Kenneth Margulies   +4 more
doaj   +2 more sources

Gene length is a pivotal feature to explain disparities in transcript capture between single transcriptome techniques

Frontiers in Bioinformatics, 2023
The scale and capability of single-cell and single-nucleus RNA-sequencing technologies are rapidly growing, enabling key discoveries and large-scale cell mapping operations.
Ricardo R. Pavan, Fabiola Diniz, Samir El-Dahr, Giovane G. Tortelote   +3 more
doaj   +1 more source

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing [PDF]

Journal of Visualized Experiments, 2021
The kidneys regulate diverse biological processes such as water, electrolyte, and acid-base homeostasis. Physiological functions of the kidney are executed by multiple cell types arranged in a complex architecture across the corticomedullary axis of the organ.
Leiz, J., Hinze, C., Boltengagen, A., Braeuning, C., Kocks, C., Rajewsky, N., Schmidt-Ott, K.M.   +6 more
openaire   +4 more sources

Recent advances in deciphering hippocampus complexity using single-cell transcriptomics

Neurobiology of Disease, 2023
Single-cell and single-nucleus RNA sequencing (scRNA-seq and snRNA-seq) technologies have emerged as revolutionary and powerful tools, which have helped in achieving significant progress in biomedical research over the last decade.
Chenxu Chang, Hongyan Zuo, Yang Li
doaj   +1 more source

Decoding DMD transcriptional networks using single‐nucleus RNA sequencing [PDF]

Proceedings of the National Academy of Sciences, 2020
Duchenne muscular dystrophy (DMD) is an X chromosome-linked disease, and it is the most common form of muscular dystrophy caused by genetic mutations in the Dmd gene (1). The Dmd gene contains 79 exons, spans 2.4 Mb, and is the single largest gene in the human genome (2).
Daniel J, Garry, Satyabrata, Das, Wuming, Gong   +2 more
openaire   +2 more sources

Comparative Analysis of Single-cell and Single-nucleus RNA-sequencing in a Rabbit Model of Retinal Detachment-related Proliferative Vitreoretinopathy

Ophthalmology Science, 2023
Purpose: Proliferative vitreoretinopathy (PVR) is the most common cause of failure of retinal reattachment surgery, and the molecular changes leading to this aberrant wound healing process are currently unknown.
Clayton P. Santiago, PhD, MS, Megan Y. Gimmen, BSc, Yuchen Lu, BSc, Minda M. McNally, MS, Leighton H. Duncan, MA, BSc, Tyler J. Creamer, PhD, Linda D. Orzolek, MS, Seth Blackshaw, PhD, Mandeep S. Singh, MD, PhD   +8 more
doaj   +1 more source

A protocol for single nucleus RNA-seq from frozen skeletal muscle

Life Science Alliance, 2023
The authors detail a method to isolate nuclei for single nucleus RNA sequencing from frozen, biobanked human skeletal muscle samples. Single-cell technologies are a method of choice to obtain vast amounts of cell-specific transcriptional information ...
Tyler GB Soule, Carly S Pontifex, Nicole Rosin, Matthew M Joel, Sukyoung Lee, Minh Dang Nguyen, Sameer Chhibber, Gerald Pfeffer   +7 more
doaj   +1 more source

Challenges and opportunities to computationally deconvolve heterogeneous tissue with varying cell sizes using single-cell RNA-sequencing datasets

Genome Biology, 2023
Deconvolution of cell mixtures in “bulk” transcriptomic samples from homogenate human tissue is important for understanding disease pathologies. However, several experimental and computational challenges impede transcriptomics-based deconvolution ...
Sean K. Maden, Sang Ho Kwon, Louise A. Huuki-Myers, Leonardo Collado-Torres, Stephanie C. Hicks, Kristen R. Maynard   +5 more
doaj   +1 more source

Optimized nucleus isolation protocol from frozen mouse tissues for single nucleus RNA sequencing application

Frontiers in Cell and Developmental Biology, 2023
The single cell RNA sequencing technique has been particularly used during the last years, allowing major discoveries. However, the widespread application of this analysis has showed limitations.
Marie-Albane Minati, Angeline Fages, Nicolas Dauguet, Nicolas Dauguet, Jingjing Zhu, Patrick Jacquemin   +5 more
doaj   +1 more source

Use of scREAD to explore and analyze single-cell and single-nucleus RNA-seq data for Alzheimer’s disease

STAR Protocols, 2021
Summary: Single-cell RNA-sequencing (scRNA-seq) and single-nucleus RNA-sequencing (snRNA-seq) studies have provided remarkable insights into understanding the molecular pathogenesis of Alzheimer's disease.
Cankun Wang, Yujia Xiang, Hongjun Fu, Qin Ma   +3 more
doaj   +1 more source