Results 21 to 30 of about 1,093 (205)
Microglia Heterogeneity in Alzheimer’s Disease: Insights From Single-Cell Technologies
Frontiers in Synaptic Neuroscience, 2021 Microglia are resident immune cells in the central nervous system and play critical roles in brain immunity, development, and homeostasis. The pathology of Alzheimer’s disease (AD) triggers activation of microglia.
Hansen Wang
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Understanding and evaluating ambiguity in single-cell and single-nucleus RNA-sequencing
, 2023 Recently, a new modification has been proposed by Hjörleifsson and Sullivan et al . to the model used to classify the splicing status of reads (as spliced (mature), unspliced (nascent), or ambiguous) in single-cell and single-nucleus RNA-seq data.
Dongze He, Charlotte Soneson, Rob Patro
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Genome Biology, 2020 Background Single-cell RNA sequencing has been widely adopted to estimate the cellular composition of heterogeneous tissues and obtain transcriptional profiles of individual cells. Multiple approaches for optimal sample dissociation and storage of single
Elena Denisenko +10 more
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Single‐nucleus RNA sequencing of plant tissues using a nanowell‐based system [PDF]
The Plant Journal, 2021 SUMMARYSingle‐cell genomics provides unprecedented potential for research on plant development and environmental responses. Here, we introduce a generic procedure for plant nucleus isolation combined with nanowell‐based library preparation. Our method enables the transcriptome analysis of thousands of individual plant nuclei.
Sunaga‐Franze, Daniele Y. +11 more
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Single-Nucleus and In Situ RNA–Sequencing Reveal Cell Topographies in the Human Pancreas [PDF]
Gastroenterology, 2019 Abstract Molecular evidence of cellular heterogeneity in the human exocrine pancreas has not been established, due to the local concentration of hydrolytic enzymes that can rapidly degrade cells and RNA upon resection. Here we innovated single-nucleus RNA sequencing protocols, and profiled more than 120,000 cells from
Luca Tosti +16 more
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Nature Communications, 2023 Formalin-fixed paraffin-embedded (FFPE) tissues constitute a vast and valuable patient material bank for clinical history and follow-up data. It is still challenging to achieve single cell/nucleus RNA (sc/snRNA) profile in FFPE tissues.
Ziye Xu +22 more
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A survey of the mouse hindbrain in the fed and fasted state using single-nucleus RNA sequencing [PDF]
Molecular Metabolism, 2021 Abstract Objective The area postrema (AP) and the nucleus tractus solitaris (NTS), located in the hindbrain, are key nuclei that sense and integrate peripheral nutritional signals and, consequently, regulate feeding behaviour.
Georgina K.C. Dowsett +9 more
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Machine learning for cell type classification from single nucleus RNA sequencing data.
PLoS ONE, 2022 With the advent of single cell/nucleus RNA sequencing (sc/snRNA-seq), the field of cell phenotyping is now a data-driven exercise providing statistical evidence to support cell type/state categorization.
Huy Le +6 more
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Isolation of nuclei from mouse white adipose tissues for single-nucleus genomics
STAR Protocols, 2021 Summary: Lipid-filled adipocytes are incompatible with droplet-based single-cell methods, such as 10x Genomics-based technology, thus restricting droplet-based single-cell analyses of adipose tissues to the stromal vascular fraction.
Elvira Laila Van Hauwaert +6 more
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Cells, 2022 Recent advances in developmental biology have been made possible by using multi-omic studies at single cell resolution. However, progress in plants has been slowed, owing to the tremendous difficulty in protoplast isolation from most plant tissues and/or
Ming-Chao Yang +4 more
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