Results 11 to 20 of about 261,642 (181)

Unveiling urethral cellular heterogeneity in menopause through single-nucleus RNA sequencing [PDF]

Frontiers in Physiology
BackgroundEstrogen homeostasis is crucial for the structure and function of the urethra, and estrogen deprivation resulting from menopause, ovariectomy, or ovarian dysfunction may lead to various urethral dysfunctions.
Jinghao Mu, Jian Xiong, Shunchang Zhou, Zhenliang Qin, Jianlin Chen, Jianlin Chen, Hui Guo, Hui Guo, Guanghui Du   +8 more
doaj   +2 more sources

Systematic comparison of single-cell and single-nucleus RNA-sequencing methods

Nature Biotechnology, 2020
The scale and capabilities of single-cell RNA-sequencing methods have expanded rapidly in recent years, enabling major discoveries and large-scale cell mapping efforts. However, these methods have not been systematically and comprehensively benchmarked.
Jiarui Ding, Xian Adiconis, Sean K. Simmons, Monika S. Kowalczyk, Cynthia C. Hession, Nemanja D. Marjanovic, Travis K. Hughes, Marc H. Wadsworth, Tyler Burks, Lan T. Nguyen, John Y. H. Kwon, Boaz Barak, William Ge, Amanda J. Kedaigle, Shaina Carroll, Shuqiang Li, Nir Hacohen, Orit Rozenblatt-Rosen, Alex K. Shalek, Alexandra-Chloé Villani, Aviv Regev, Joshua Z. Levin   +21 more
openaire   +5 more sources

Comparative analysis of nuclei isolation methods for brain single-nucleus RNA sequencing. [PDF]

Cell Rep Methods
SUMMARYSingle-nucleus RNA sequencing (snRNA-seq) enables resolving cellular heterogeneity in complex tissues. snRNA-seq overcomes limitations of traditional single-cell RNA-seq by using nuclei instead of cells, allowing to utilize frozen tissues and difficult-to-isolate cell types.
Kersey HN, Acri DJ, Dabin LC, Hartigan KA, Mustaklem R, Park JH, Kim J.   +6 more
europepmc   +3 more sources

Miniaturization of Smart-seq2 for Single-Cell and Single-Nucleus RNA Sequencing

STAR Protocols, 2020
Summary: This protocol presents a plate-based workflow to perform RNA sequencing analysis of single cells/nuclei using Smart-seq2. We describe (1) the dissociation procedures for cell/nucleus isolation from the mouse brain and human organoids, (2) the ...
Baptiste N. Jaeger, Emilio Yángüez, Lorenzo Gesuita, Annina Denoth-Lippuner, Merit Kruse, Theofanis Karayannis, Sebastian Jessberger   +6 more
doaj   +4 more sources

Perspectives on single-nucleus RNA sequencing in different cell types and tissues [PDF]

Journal of Pathology and Translational Medicine, 2023
Single-cell RNA sequencing has become a powerful and essential tool for delineating cellular diversity in normal tissues and alterations in disease states.
Nayoung Kim, Huiram Kang, Areum Jo, Seung-Ah Yoo, Hae-Ock Lee   +4 more
doaj   +3 more sources

Sex-specific proximal tubular cell differentiation pathways identified by single-nucleus RNA sequencing

Scientific Reports, 2023
Postnatal kidney growth is substantial and involves expansion in kidney tubules without growth of new nephrons, which are the functional units of the kidney. Proliferation and differentiation pathways underpinning nephron elongation are not well defined.
Yueh-An Lu, Tanya Smith, Sumukh Deshpande, Chia-Te Liao, Bnar Talabani, Irina Grigorieva, Anna Mason, Robert Andrews, Timothy Bowen, Philip R. Taylor, Donald Fraser   +10 more
doaj   +3 more sources

Benchmarking of computational demultiplexing methods for single-nucleus RNA sequencing data. [PDF]

Brief Bioinform
Abstract Single-nucleus RNA sequencing (snRNA-Seq) has transformed our understanding of complex tissues, providing insights into cellular composition and heterogeneity in gene expression between cells, and their alterations in development and disease. High costs however constrain the number of samples analysed.
Fu Y, Youness M, Virzì A, Song X, Tubeeckx MRL, De Keulenaer GW, Heidbuchel H, Segers VFM, Sipido KR, Thienpont B, Roderick HL.   +10 more
europepmc   +4 more sources

A simplified preparation method for single-nucleus RNA-sequencing using long-term frozen brain tumor tissues [PDF]

Scientific Reports
Single-cell RNA-sequencing has provided intriguing new insights into research areas such as developmental processes and tumor heterogeneity. Most approaches, however, rely on the availability of fresh surgical specimens, thereby dramatically reducing the
Kati J. Ernst, Konstantin Okonechnikov, Josephine Bageritz, Ashwyn A. Perera, Jan-Philipp Mallm, Andrea Wittmann, Kendra K. Maaß, Svenja Leible, Michael Boutros, Stefan M. Pfister, Marc Zuckermann, David T. W. Jones   +11 more
doaj   +2 more sources

Single-nucleus RNA-sequencing of autosomal dominant Alzheimer disease and risk variant carriers

Nature Communications, 2023
Genetic studies of Alzheimer disease (AD) have prioritized variants in genes related to the amyloid cascade, lipid metabolism, and neuroimmune modulation. However, the cell-specific effect of variants in these genes is not fully understood.
Logan Brase, Shih-Feng You, Ricardo D’Oliveira Albanus, Jorge L. Del-Aguila, Yaoyi Dai, Brenna C. Novotny, Carolina Soriano-Tarraga, Taitea Dykstra, Maria Victoria Fernandez, John P. Budde, Kristy Bergmann, John C. Morris, Randall J. Bateman, Richard J. Perrin, Eric McDade, Chengjie Xiong, Alison M. Goate, Martin Farlow, Dominantly Inherited Alzheimer Network (DIAN), Greg T. Sutherland, Jonathan Kipnis, Celeste M. Karch, Bruno A. Benitez, Oscar Harari   +23 more
doaj   +4 more sources

Single‐Nucleus RNA‐Sequencing in Brain Tissue

Current Protocols, 2023
AbstractNeuroscience research greatly benefits from single‐cell sequencing technologies, which can reveal transcriptional alterations on a cellular level. However, preparing single‐cell suspensions is technically challenging, requires experience, and has several limitations that can influence the transcriptional readout. Performing sequencing of single
Rebecca Waag, Johannes Bohacek
openaire   +4 more sources