Results 11 to 20 of about 165,721 (244)

Single-nucleus RNA-sequencing in pre-cellularization Drosophila melanogaster embryos. [PDF]

PLoS ONE, 2022
Our current understanding of the regulation of gene expression in the early Drosophila melanogaster embryo comes from observations of a few genes at a time, as with in situ hybridizations, or observation of gene expression levels without regards to ...
Ashley R Albright, Michael R Stadler, Michael B Eisen   +2 more
doaj   +3 more sources

Simultaneous single-nucleus RNA sequencing and single-nucleus ATAC sequencing of neuroblastoma cell lines

Scientific Data
Neuroblastoma is the most common extracranial solid tumor in children, and a leading cause of childhood cancer deaths. All neuroblastomas arise from neural crest-derived sympathetic neuronal progenitors, but numerous mutations, the most common of which ...
Richard A. Guyer, Jessica L. Mueller, Nicole Picard, Allan M. Goldstein   +3 more
doaj   +5 more sources

Miniaturization of Smart-seq2 for Single-Cell and Single-Nucleus RNA Sequencing

STAR Protocols, 2020
Summary: This protocol presents a plate-based workflow to perform RNA sequencing analysis of single cells/nuclei using Smart-seq2. We describe (1) the dissociation procedures for cell/nucleus isolation from the mouse brain and human organoids, (2) the ...
Baptiste N. Jaeger, Emilio Yángüez, Lorenzo Gesuita, Annina Denoth-Lippuner, Merit Kruse, Theofanis Karayannis, Sebastian Jessberger   +6 more
doaj   +5 more sources

Perspectives on single-nucleus RNA sequencing in different cell types and tissues [PDF]

Journal of Pathology and Translational Medicine, 2023
Single-cell RNA sequencing has become a powerful and essential tool for delineating cellular diversity in normal tissues and alterations in disease states.
Nayoung Kim, Huiram Kang, Areum Jo, Seung-Ah Yoo, Hae-Ock Lee   +4 more
doaj   +3 more sources

Deciphering deep-sea chemosynthetic symbiosis by single-nucleus RNA-sequencing

eLife, 2023
Bathymodioline mussels dominate deep-sea methane seep and hydrothermal vent habitats and obtain nutrients and energy primarily through chemosynthetic endosymbiotic bacteria in the bacteriocytes of their gill.
Hao Wang, Kai He, Huan Zhang, Quanyong Zhang, Lei Cao, Jing Li, Zhaoshan Zhong, Hao Chen, Li Zhou, Chao Lian, Minxiao Wang, Kai Chen, Pei-Yuan Qian, Chaolun Li   +13 more
doaj   +4 more sources

Single‐Nucleus RNA‐Sequencing in Brain Tissue

Current Protocols, 2023
AbstractNeuroscience research greatly benefits from single‐cell sequencing technologies, which can reveal transcriptional alterations on a cellular level. However, preparing single‐cell suspensions is technically challenging, requires experience, and has several limitations that can influence the transcriptional readout. Performing sequencing of single
Rebecca Waag, Johannes Bohacek
openaire   +3 more sources

Protocol for single-nucleus RNA sequencing of adult C. elegans neurons. [PDF]

STAR Protoc
Single-nucleus sequencing, specifically, remains the gold standard for characterizing difficult-to-dissociate cell types, such as neurons in complex brain tissue, both in mammals and in Caenorhabditis elegans. Here, we present a protocol for single-nucleus RNA sequencing of adult C. elegans neurons. This protocol describes the steps for creating a high-
St Ange J, Kaletsky R, Morillo KS, Murphy CT.   +3 more
europepmc   +4 more sources

Assessing Markovian and Delay Models for Single-Nucleus RNA Sequencing

Bulletin of Mathematical Biology, 2023
The serial nature of reactions involved in the RNA life-cycle motivates the incorporation of delays in models of transcriptional dynamics. The models couple a transcriptional process to a fairly general set of delayed monomolecular reactions with no feedback.
Gorin, Gennady, Yoshida, Shawn, Pachter, Lior   +2 more
openaire   +4 more sources

Decoding DMD transcriptional networks using single‐nucleus RNA sequencing [PDF]

Proceedings of the National Academy of Sciences, 2020
Duchenne muscular dystrophy (DMD) is an X chromosome-linked disease, and it is the most common form of muscular dystrophy caused by genetic mutations in the Dmd gene (1). The Dmd gene contains 79 exons, spans 2.4 Mb, and is the single largest gene in the human genome (2).
Daniel J, Garry, Satyabrata, Das, Wuming, Gong   +2 more
openaire   +2 more sources

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing [PDF]

Journal of Visualized Experiments, 2021
The kidneys regulate diverse biological processes such as water, electrolyte, and acid-base homeostasis. Physiological functions of the kidney are executed by multiple cell types arranged in a complex architecture across the corticomedullary axis of the organ.
Leiz, J., Hinze, C., Boltengagen, A., Braeuning, C., Kocks, C., Rajewsky, N., Schmidt-Ott, K.M.   +6 more
openaire   +3 more sources