Results 11 to 20 of about 1,093 (205)
Miniaturization of Smart-seq2 for Single-Cell and Single-Nucleus RNA Sequencing
STAR Protocols, 2020 Summary: This protocol presents a plate-based workflow to perform RNA sequencing analysis of single cells/nuclei using Smart-seq2. We describe (1) the dissociation procedures for cell/nucleus isolation from the mouse brain and human organoids, (2) the ...
Baptiste N. Jaeger +6 more
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Scientific DataNeuroblastoma is the most common extracranial solid tumor in children, and a leading cause of childhood cancer deaths. All neuroblastomas arise from neural crest-derived sympathetic neuronal progenitors, but numerous mutations, the most common of which ...
Richard A. Guyer +3 more
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Perspectives on single-nucleus RNA sequencing in different cell types and tissues [PDF]
Journal of Pathology and Translational Medicine, 2023 Single-cell RNA sequencing has become a powerful and essential tool for delineating cellular diversity in normal tissues and alterations in disease states.
Nayoung Kim +4 more
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Deciphering deep-sea chemosynthetic symbiosis by single-nucleus RNA-sequencing
eLife, 2023 Bathymodioline mussels dominate deep-sea methane seep and hydrothermal vent habitats and obtain nutrients and energy primarily through chemosynthetic endosymbiotic bacteria in the bacteriocytes of their gill.
Hao Wang +13 more
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snPATHO-seq: A Detailed Protocol for Single Nucleus RNA Sequencing From FFPE
Bio-ProtocolFormalin-fixed paraffin-embedded (FFPE) samples remain an underutilized resource in single-cell omics due to RNA degradation from formalin fixation. Here, we present snPATHO-seq, a robust and adaptable approach that enables the generation of high-quality
Wani Arjumand +4 more
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Single‐Nucleus RNA‐Sequencing in Brain Tissue
Current Protocols, 2023 AbstractNeuroscience research greatly benefits from single‐cell sequencing technologies, which can reveal transcriptional alterations on a cellular level. However, preparing single‐cell suspensions is technically challenging, requires experience, and has several limitations that can influence the transcriptional readout. Performing sequencing of single
Rebecca Waag, Johannes Bohacek
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Assessing Markovian and Delay Models for Single-Nucleus RNA Sequencing
Bulletin of Mathematical Biology, 2023 The serial nature of reactions involved in the RNA life-cycle motivates the incorporation of delays in models of transcriptional dynamics. The models couple a transcriptional process to a fairly general set of delayed monomolecular reactions with no feedback.
Gorin, Gennady +2 more
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Decoding DMD transcriptional networks using single‐nucleus RNA sequencing [PDF]
Proceedings of the National Academy of Sciences, 2020 Duchenne muscular dystrophy (DMD) is an X chromosome-linked disease, and it is the most common form of muscular dystrophy caused by genetic mutations in the Dmd gene (1). The Dmd gene contains 79 exons, spans 2.4 Mb, and is the single largest gene in the human genome (2).
Daniel J, Garry +2 more
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Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing [PDF]
Journal of Visualized Experiments, 2021 The kidneys regulate diverse biological processes such as water, electrolyte, and acid-base homeostasis. Physiological functions of the kidney are executed by multiple cell types arranged in a complex architecture across the corticomedullary axis of the organ.
Leiz, J. +6 more
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Experimental & Molecular Medicine, 2022 AbstractSingle-cell ribonucleic acid (RNA) sequencing (scRNA-seq) is an effective technique for estimating the cellular composition and transcriptional profiles of individual cells from fresh tissue. Single-nucleus RNA sequencing (snRNA-seq) is necessary to perform this type of analysis in frozen or difficult-to-dissociate tissues, which cannot be ...
Jin-Mi Oh +6 more
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