Results 71 to 80 of about 1,093 (205)

Understanding of the characteristics of fibroblasts in ischemic cardiomyopathy using single- nucleus RNA sequencing

Scientific Reports
Abstract Fibroblasts play a crucial role in the development and progression of heart failure in many heart-related diseases. However, the contributions of fibroblast and its subtypes to the pathogenesis of ischemic cardiomyopathy (ICM) remain unclear.
Qianyuan Zhang, Ziwen Cai, Yongqiao Zhang   +2 more
openaire   +3 more sources

Unveiling urethral cellular heterogeneity in menopause through single-nucleus RNA sequencing

Frontiers in Physiology
BackgroundEstrogen homeostasis is crucial for the structure and function of the urethra, and estrogen deprivation resulting from menopause, ovariectomy, or ovarian dysfunction may lead to various urethral dysfunctions.
Jinghao Mu, Jian Xiong, Shunchang Zhou, Zhenliang Qin, Jianlin Chen, Jianlin Chen, Hui Guo, Hui Guo, Guanghui Du   +8 more
doaj   +1 more source

Epigenetic heterogeneity and plasticity in therapy‐induced tumor states through single‐cell multi‐omics

Molecular Oncology, EarlyView.
Single‐cell multi‐omics reveals epigenetic heterogeneity across therapy‐adaptive tumor states, including quiescent/dormant, drug‐tolerant persister, and EMT‐like phenotypes. By linking regulatory features with state‐associated biomarkers, these approaches inform biomarker‐guided therapeutic strategies for evolving tumors.
Hee Jung Kim, Hwiyeong Lee, Jin Hong, Daechan Park   +3 more
wiley   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Comparative analysis of nuclei isolation methods for brain single-nucleus RNA sequencing

Cell Reports Methods
SUMMARY Single-nucleus RNA sequencing (snRNA-seq) enables resolving cellular heterogeneity in complex tissues. snRNA-seq overcomes limitations of traditional single-cell RNA-seq by using nuclei instead of cells, allowing to utilize frozen tissues and difficult-to-isolate cell types.
Holly N. Kersey, Dominic J. Acri, Luke C. Dabin, Kelly Hartigan, Richard Mustaklem, Jung Hyun Park, Jungsu Kim   +6 more
openaire   +3 more sources

A single-nucleus RNA sequencing atlas of the postnatal retina of the shark Scyliorhinus canicula [PDF]

Scientific Data
Abstract The retina, whose basic cellular structure is highly conserved across vertebrates, constitutes an accessible system for studying the central nervous system. In recent years, single-cell RNA-sequencing studies have uncovered cellular diversity in the retina of a variety of species, providing new insights on ...
Nicolás Vidal-Vázquez, Ismael Hernández-Núñez, Pablo Carballo-Pacoret, Sarah Salisbury, Paula R. Villamayor, Francisca Hervas-Sotomayor, Xuefei Yuan, Francesco Lamanna, Céline Schneider, Julia Schmidt, Sylvie Mazan, Henrik Kaessmann, Fátima Adrio, Diego Robledo, Antón Barreiro-Iglesias, Eva Candal   +15 more
openaire   +3 more sources

A simplified preparation method for single-nucleus RNA-sequencing using long-term frozen brain tumor tissues

Scientific Reports
Single-cell RNA-sequencing has provided intriguing new insights into research areas such as developmental processes and tumor heterogeneity. Most approaches, however, rely on the availability of fresh surgical specimens, thereby dramatically reducing the
Kati J. Ernst, Konstantin Okonechnikov, Josephine Bageritz, Ashwyn A. Perera, Jan-Philipp Mallm, Andrea Wittmann, Kendra K. Maaß, Svenja Leible, Michael Boutros, Stefan M. Pfister, Marc Zuckermann, David T. W. Jones   +11 more
doaj   +1 more source

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

PARP inhibitors induce a senescence phenotype in non‐small cell lung carcinoma cell lines

FEBS Open Bio, EarlyView.
Talazoparib is the most potent inducer of senescence among different PARP1 inhibitors in human NSCLC cells. In the absence of PARP, no senescence phenotype was observed, demonstrating that PARP1 is necessary for the induction of senescence by this inhibitor.
Camille Huart, Manon Van den Abbeel, Christoph Schifflers, Karim Bouhjar, Andrea Scarmelotto, Alexis Khelfi, Anne‐Catherine Wera, Carine Michiels   +7 more
wiley   +1 more source

Comprehensive Multiomic Analysis Reveals Metabolic Reprogramming Underlying Human Fontan‐Associated Liver Disease

Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease
Background The Fontan operation is the current standard of care for single‐ventricle congenital heart disease. Almost all patients with Fontan operation develop liver fibrosis at a young age, known as Fontan‐associated liver disease (FALD).
Rasheed Sule, Po Hu, Clarissa Shoffler, Christopher Petucci, Benjamin J. Wilkins, Jack Rychik, Liming Pei   +6 more
doaj   +1 more source