Insights into HIV uncoating from single-particle imaging techniques. [PDF]
Human immunodeficiency virus (HIV) is the most extensively researched human pathogen. Despite this massive scientific endeavour, several fundamental viral processes remain enigmatic. One such critical process is uncoating-the event that releases the viral genome from the proteinaceous shell of the capsid during infection.
Zhang MJ +3 more
europepmc +3 more sources
Biological single-particle imaging using XFELs – towards the next resolution revolution [PDF]
Dominik Oberthür
doaj +2 more sources
Single Particle X-ray Diffractive Imaging [PDF]
In nanotechnology, strategies for the creation and manipulation of nanoparticles in the gas phase are critically important for surface modification and substrate-free characterization. Recent coherent diffractive imaging with intense femtosecond X-ray pulses has verified the capability of single-shot imaging of nanoscale objects at suboptical ...
Bogan, M. J. +17 more
openaire +3 more sources
A reconstruction algorithm for single-particle diffraction imaging experiments [PDF]
52 pages, 17 figures and uses algorithm2e package v4: split first section into 2.
Loh, Duane Ne-Te, Elser, Veit
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The role of transient resonances for ultra-fast imaging of single sucrose nanoclusters
X-ray free electron lasers provide high photon flux to explore single particle diffraction imaging of biological samples. Here the authors present dynamic electronic structure calculations and benchmark them to single-particle XFEL diffraction data of ...
Phay J. Ho +23 more
doaj +1 more source
3D printed devices and infrastructure for liquid sample delivery at the European XFEL
The Sample Environment and Characterization (SEC) group of the European X-ray Free-Electron Laser (EuXFEL) develops sample delivery systems for the various scientific instruments, including systems for the injection of liquid samples that enable serial ...
Mohammad Vakili +20 more
doaj +1 more source
Tracking Image Correlation: Combining Single-Particle Tracking and Image Correlation [PDF]
The interactions and coordination of biomolecules are crucial for most cellular functions. The observation of protein interactions in live cells may provide a better understanding of the underlying mechanisms. After fluorescent labeling of the interacting partners and live-cell microscopy, the colocalization is generally analyzed by quantitative global
Dupont, A. +3 more
openaire +2 more sources
Modular Imaging Scaffold for Single-Particle Electron Microscopy
Technological breakthroughs in electron microscopy (EM) have made it possible to solve structures of biological macromolecular complexes and to raise novel challenges, specifically related to sample preparation and heterogeneous macromolecular assemblies such as DNA-protein, protein-protein, and membrane protein assemblies.
Aissaoui, Nesrine +13 more
openaire +5 more sources
Emerging Themes in CryoEM─Single Particle Analysis Image Processing
Cryo-electron microscopy (CryoEM) has become a vital technique in structural biology. It is an interdisciplinary field that takes advantage of advances in biochemistry, physics, and image processing, among other disciplines. Innovations in these three basic pillars have contributed to the boosting of CryoEM in the past decade.
Vilas, Jose Luis +2 more
openaire +2 more sources
Microlens-Assisted Light-Scattering Imaging of Plasmonic Nanoparticles at the Single Particle Level
We present a microlens-assisted imaging approach to record the scattering light of plasmonic nanoparticles at the single particle level. The microlens can significantly enhance the backscattering of visible light from individual plasmonic nanoparticles ...
Pengcheng Zhang +3 more
doaj +1 more source

