Results 31 to 40 of about 173,038 (236)

Microlens-Assisted Light-Scattering Imaging of Plasmonic Nanoparticles at the Single Particle Level

Biosensors, 2023
We present a microlens-assisted imaging approach to record the scattering light of plasmonic nanoparticles at the single particle level. The microlens can significantly enhance the backscattering of visible light from individual plasmonic nanoparticles ...
Pengcheng Zhang, Tingting Zhan, Sha Xue, Hui Yang   +3 more
doaj   +1 more source

Isotropic resolution in fluorescence imaging by single particle reconstruction [PDF]

2016 IEEE 13th International Symposium on Biomedical Imaging (ISBI), 2016
Low axial resolution is a major limitation of fluorescence imaging modalities. We propose a methodology to achieve high isotropic resolution by reconstructing fluorescence volumes from observations of multiple particle replicates with different orientations.
Denis Fortun, Paul Guichard, Ning Chu, Michael Unser   +3 more
openaire   +1 more source

Megahertz single-particle imaging at the European XFEL [PDF]

Communications Physics, 2020
AbstractThe emergence of high repetition-rate X-ray free-electron lasers (XFELs) powered by superconducting accelerator technology enables the measurement of significantly more experimental data per day than was previously possible. The European XFEL is expected to provide 27,000 pulses per second, over two orders of magnitude more than any other XFEL.
Egor Sobolev, Sergei Zolotarev, Klaus Giewekemeyer, Johan Bielecki, Kenta Okamoto, Hemanth K. N. Reddy, Jakob Andreasson, Kartik Ayyer, Imrich Barak, Sadia Bari, Anton Barty, Richard Bean, Sergey Bobkov, Henry N. Chapman, Grzegorz Chojnowski, Benedikt J. Daurer, Katerina Dörner, Tomas Ekeberg, Leonie Flückiger, Oxana Galzitskaya, Luca Gelisio, Steffen Hauf, Brenda G. Hogue, Daniel A. Horke, Ahmad Hosseinizadeh, Vyacheslav Ilyin, Chulho Jung, Chan Kim, Yoonhee Kim, Richard A. Kirian, Henry Kirkwood, Olena Kulyk, Jochen Küpper, Romain Letrun, N. Duane Loh, Kristina Lorenzen, Marc Messerschmidt, Kerstin Mühlig, Abbas Ourmazd, Natascha Raab, Andrei V. Rode, Max Rose, Adam Round, Takushi Sato, Robin Schubert, Peter Schwander, Jonas A. Sellberg, Marcin Sikorski, Alessandro Silenzi, Changyong Song, John C. H. Spence, Stephan Stern, Jolanta Sztuk-Dambietz, Anthon Teslyuk, Nicusor Timneanu, Martin Trebbin, Charlotte Uetrecht, Britta Weinhausen, Garth J. Williams, P. Lourdu Xavier, Chen Xu, Ivan A. Vartanyants, Victor S. Lamzin, Adrian Mancuso, Filipe R. N. C. Maia   +64 more
openaire   +13 more sources

Single Particle Imaging with FEL Using Photon Correlations [PDF]

, 2020
AbstractScattering experiments with femtosecond high-intensity free-electron laser pulses provide a new route to macromolecular structure determination without the need for crystallization at low material usage. In these experiments, the X-ray pulses are scattered with high repetition on a stream of identical single biomolecules and the scattered ...
von Ardenne, Benjamin, Grubmüller, Helmut, Salditt, Tim, Egner, Alexander, Luke, D. Russell   +4 more
openaire   +4 more sources

Solution of the phase problem for coherent scattering from a disordered system of identical particles

New Journal of Physics, 2013
While the implementation of single-particle coherent diffraction imaging for non-crystalline particles is complicated by current limitations on photon flux, hit rate and sample delivery, the concept of many-particle coherent diffraction imaging offers an
R P Kurta, R Dronyak, M Altarelli, E Weckert, I A Vartanyants   +4 more
doaj   +1 more source

Selection and orientation of different particles in single particle imaging [PDF]

Journal of Structural Biology, 2013
The short pulses of X-ray free electron lasers can produce diffraction patterns with structural information before radiation damage destroys the particle. The particles are injected into the beam in random orientations and they should be identical. However, in real experimental conditions it is not always possible to have identical particles.
Tegze, Miklós, Bortel, Gábor
openaire   +3 more sources

Emerging Themes in CryoEM─Single Particle Analysis Image Processing

Chemical Reviews, 2022
Cryo-electron microscopy (CryoEM) has become a vital technique in structural biology. It is an interdisciplinary field that takes advantage of advances in biochemistry, physics, and image processing, among other disciplines. Innovations in these three basic pillars have contributed to the boosting of CryoEM in the past decade.
Vilas, Jose Luis, Carazo, Jose Maria, Sorzano, Carlos Oscar S.   +2 more
openaire   +2 more sources

The linac coherent light source single particle imaging road map

Structural Dynamics, 2015
Intense femtosecond x-ray pulses from free-electron laser sources allow the imaging of individual particles in a single shot. Early experiments at the Linac Coherent Light Source (LCLS) have led to rapid progress in the field and, so ...
A. Aquila, A. Barty, C. Bostedt, S. Boutet, G. Carini, D. dePonte, P. Drell, S. Doniach, K. H. Downing, T. Earnest, H. Elmlund, V. Elser, M. Gühr, J. Hajdu, J. Hastings, S. P. Hau-Riege, Z. Huang, E. E. Lattman, F. R. N. C. Maia, S. Marchesini, A. Ourmazd, C. Pellegrini, R. Santra, I. Schlichting, C. Schroer, J. C. H. Spence, I. A. Vartanyants, S. Wakatsuki, W. I. Weis, G. J. Williams   +29 more
doaj   +1 more source

The structure of tick-borne encephalitis virus determined at X-ray free-electron lasers. Simulations

Journal of Synchrotron Radiation, 2023
The study of virus structures by X-ray free-electron lasers (XFELs) has attracted increased attention in recent decades. Such experiments are based on the collection of 2D diffraction patterns measured at the detector following the application of ...
Dameli Assalauova, Ivan A. Vartanyants
doaj   +1 more source

Tracking Image Correlation: Combining Single-Particle Tracking and Image Correlation [PDF]

Biophysical Journal, 2013
The interactions and coordination of biomolecules are crucial for most cellular functions. The observation of protein interactions in live cells may provide a better understanding of the underlying mechanisms. After fluorescent labeling of the interacting partners and live-cell microscopy, the colocalization is generally analyzed by quantitative global
Dupont, A., Stirnnagel, K., Lindemann, D., Lamb, D.C.   +3 more
openaire   +2 more sources