Results 41 to 50 of about 173,386 (236)

Tracking Image Correlation: Combining Single-Particle Tracking and Image Correlation [PDF]

Biophysical Journal, 2013
The interactions and coordination of biomolecules are crucial for most cellular functions. The observation of protein interactions in live cells may provide a better understanding of the underlying mechanisms. After fluorescent labeling of the interacting partners and live-cell microscopy, the colocalization is generally analyzed by quantitative global
Dupont, A., Stirnnagel, K., Lindemann, D., Lamb, D.C.   +3 more
openaire   +2 more sources

Single-molecule and particle detection on true portable microscopy platforms

Sensors and Actuators Reports, 2022
Point-of-care technologies (POCT) that enable early disease detection and therapeutic monitoring are crucial for the next generation of diagnostics and personalized medicine.
Lydia Skolrood, Yan Wang, Shengwei Zhang, Qingshan Wei   +3 more
doaj   +1 more source

Denoising and covariance estimation of single particle cryo-EM images [PDF]

Journal of Structural Biology, 2016
Revision for ...
Bhamre, Tejal, Zhang, Teng, Singer, Amit
openaire   +4 more sources

Local Substrate Heterogeneity Influences Electrochemical Activity of TEM Grid-Supported Battery Particles

Frontiers in Chemistry, 2021
Understanding how particle size and morphology influence ion insertion dynamics is critical for a wide range of electrochemical applications including energy storage and electrochromic smart windows.
Christina Cashen, R. Colby Evans, Zach N. Nilsson, Justin B. Sambur   +3 more
doaj   +1 more source

Clustering and averaging of images in single-particle analysis.

Genome informatics. Workshop on Genome Informatics, 2000
Single particle analysis is a straightforward method for studying the structures of macromolecules that cannot be crystallized. It builds three-dimensional structures of particles by estimating the projection angles of their randomly oriented electron-microscopic images. The existing methods divide the images into clusters, build class averages for the
Asai, Kiyoshi, Sato, Chikara, Ueno, Yutaka, Takahashi, Katsutoshi   +3 more
openaire   +2 more sources

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Computational study of diffraction image formation from XFEL irradiated single ribosome molecule

Scientific Reports
Single particle imaging at atomic resolution is perhaps one of the most desired goals for ultrafast X-ray science with X-ray free-electron lasers.
Michal Stransky, Juncheng E, Zoltan Jurek, Robin Santra, Richard Bean, Beata Ziaja, Adrian P. Mancuso   +6 more
doaj   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Organizing the interface—Plasma membrane architecture and receptor dynamics in virus‐cell interactions

FEBS Letters, EarlyView.
Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley   +1 more source

Principles of cryo-EM single-particle image processing [PDF]

Microscopy, 2015
Single-particle reconstruction is the process by which 3D density maps are obtained from a set of low-dose cryo-EM images of individual macromolecules. This review considers the fundamental principles of this process and the steps in the overall workflow for single-particle image processing.
openaire   +2 more sources