Results 71 to 80 of about 1,316,697 (275)

Visualizing aerosol-particle injection for diffractive-imaging experiments

, 2015
Delivering sub-micrometer particles to an intense x-ray focus is a crucial aspect of single-particle diffractive-imaging experiments at x-ray free-electron lasers.
Awel, Salah, Chapman, Henry N., Eckerskorn, Niko, Horke, Daniel A., Kirian, Richard A., Küpper, Jochen, Rode, Andrei V., Wiedorn, Max   +7 more
core   +1 more source

Orientational Imaging of Subwavelength Au Particles with Higher Order Laser Modes [PDF]

, 2006
We present a new method for the imaging of single metallic nanoparticles that provides information about their shape and orientation. Using confocal microscopy in combination with higher order laser modes, scattering images of individual particles are ...
Failla, Antonio Virgilio, Hartschuh, Achim, Meixner, Alfred Johann, Qian, Hui, Qian, Huihong   +4 more
core   +1 more source

Characterizing gas flow from aerosol particle injectors

, 2017
A novel methodology for measuring gas flow from small orifices or nozzles into vacuum is presented. It utilizes a high-intensity femtosecond laser pulse to create a plasma within the gas plume produced by the nozzle, which is imaged by a microscope ...
Horke, Daniel, Küpper, Jochen, Roth, Nils, Worbs, Lena   +3 more
core   +2 more sources

4D Imaging and Diffraction Dynamics of Single-Particle Phase Transition in Heterogeneous Ensembles [PDF]

, 2014
In this Letter, we introduce conical-scanning dark-field imaging in four-dimensional (4D) ultrafast electron microscopy to visualize single-particle dynamics of a polycrystalline ensemble undergoing phase transitions.
Ahmed H. Zewail, Appavoo K., Appavoo K., Babkin E. V., Baik J. M., Balandin A. A., Barwick B., Baum P., Berglund C. N., Cao J., Cavalleri A., Cavalleri A., Cavalleri A., Chae B.-G., Chen J., Coy H., Dingley D. J., Dingley D. J., Duda J. C., Flannigan D. J., Fu D., Gavini A., Grinolds M. S., Hada M., Haihua Liu, Hawkes P. W., Jackson J. D., Jau Tang, Kana Kana J. B., Knoll M., Kwon O.-H., Kwon O.-H., Kwon O.-H., Kübler C., Leroux Ch., Liu H. H., Lobastov V. A., Lopez R., Lysenko S., Lysenko S., Morin F. J., Nair R. R., Narayan J., Norris P. M, Oh D.-W., Oh-Hoon Kwon, Okimura K., Park H. S., Pergament A., Picard S., Pierson H. O., Poulsen H. F., Prasher R., Schmidt S., Van der Veen R. M., Wang Z. Q., Williams D. B., Wu J. M., Yao T., Yurtsever A., Zeuner S., Zewail A. H., Zewail A. H.   +62 more
core   +1 more source

Revealing the structure of land plant photosystem II: the journey from negative‐stain EM to cryo‐EM

FEBS Letters, EarlyView.
Advances in cryo‐EM have revealed the detailed structure of Photosystem II, a key protein complex driving photosynthesis. This review traces the journey from early low‐resolution images to high‐resolution models, highlighting how these discoveries deepen our understanding of light harvesting and energy conversion in plants.
Roman Kouřil
wiley   +1 more source

Computational study of diffraction image formation from XFEL irradiated single ribosome molecule

Scientific Reports
Single particle imaging at atomic resolution is perhaps one of the most desired goals for ultrafast X-ray science with X-ray free-electron lasers.
Michal Stransky, Juncheng E, Zoltan Jurek, Robin Santra, Richard Bean, Beata Ziaja, Adrian P. Mancuso   +6 more
doaj   +1 more source

Purification and electron cryomicroscopy of coronavirus particles. [PDF]

, 2008
Intact, enveloped coronavirus particles vary widely in size and contour, and are thus refractory to study by traditional structural means such as X-ray crystallography. Electron microscopy (EM) overcomes some problems associated with particle variability
Adair, Brian D, Buchmeier, Michael J, Neuman, Benjamin W, Yeager, Mark   +3 more
core   +1 more source

Mapping the evolution of mitochondrial complex I through structural variation

FEBS Letters, EarlyView.
Respiratory complex I (CI) is crucial for bioenergetic metabolism in many prokaryotes and eukaryotes. It is composed of a conserved set of core subunits and additional accessory subunits that vary depending on the organism. Here, we categorize CI subunits from available structures to map the evolution of CI across eukaryotes. Respiratory complex I (CI)
Dong‐Woo Shin, Tingting Chen, James A. Letts   +2 more
wiley   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Orientation Determination in Single Particle X-ray Coherent Diffraction Imaging Experiments

, 2013
Single particle diffraction imaging experiments at free-electron lasers (FEL) have a great potential for structure determination of reproducible biological specimens that can not be crystallized.
Vartanyants, I. A., Yefanov, O. M.
core   +1 more source