Results 41 to 50 of about 58,343 (263)

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

FEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow, Isabel Brandtjen, Yaxin Mo, Melissa Gulley, Louis Osorio, Sagar Kudchodkar, Nandita C. Jhumur, Christine C. Roberts, Lisa K. Denzin, David I. Shreiber, Biju Parekkadan, Hao Lin, Joel N. Maslow   +12 more
wiley   +1 more source

Detector of microwave photon pairs based on a Josephson photomultiplier

Physical Review Research
We propose a viable design of a microwave two-photon threshold detector. In essence, the considered scheme is an extension of the existing single-photon detector—a Josephson photomultiplier (JPM)—an absorbing microwave detector based on a capacitively ...
E. V. Stolyarov, R. A. Baskov
doaj   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

Sixteen-Pixel NbN Nanowire Single Photon Detector Coupled With 300-μm Fiber

IEEE Photonics Journal, 2020
Niobium nitride (NbN) nanowires have a high repetition rate and efficiency, making them ideal for superconducting nanowire single photon detectors (SNSPDs).
Qi Chen, Biao Zhang, Labao Zhang, Rui Ge, Ruiying Xu, Yang Wu, Xuecou Tu, Xiaoqing Jia, Danfeng Pan, Lin Kang, Jian Chen, Peiheng Wu   +11 more
doaj   +1 more source

Identification and characterization of a gene conferring stress resistance in Escherichia coli and cyanobacteria

FEBS Open Bio, EarlyView.
Functional screening identified PcSyn14890, a cyanobacteria‐specific protein that enhances growth and stress resistance in E. coli and Synechocystis PCC6803. Although we expected it to function as a molecular chaperone, it was unable to protect against thermal aggregation of GAPDH.
Akiyo Yamada, Takaaki Tanaka, Manaka Uehara, Keigo Nakano, Ken Morishima, Rintaro Inoue, Masaaki Sugiyama, Masafumi Yohda   +7 more
wiley   +1 more source

Photon-number resolving detector based on a cascade of waveguide-coupled quantum emitters

JPhys Photonics
We investigate the operation of a photon-number-resolving (PNR) detector consisting of a cascade of waveguide-coupled Λ-type emitters, where each waveguide-coupled emitter extracts a single photon from the input light and sends it to a single-photon ...
Abdolreza Pasharavesh, Sai Sreesh Venuturumilli, Michal Bajcsy   +2 more
doaj   +1 more source

Confocal-based fluorescence fluctuation spectroscopy with a SPAD array detector

Light: Science & Applications, 2021
The combination of confocal laser-scanning microscopy (CLSM) and fluorescence fluctuation spectroscopy (FFS) is a powerful tool in studying fast, sub-resolution biomolecular processes in living cells.
Eli Slenders, Marco Castello, Mauro Buttafava, Federica Villa, Alberto Tosi, Luca Lanzanò, Sami Valtteri Koho, Giuseppe Vicidomini   +7 more
doaj   +1 more source

Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells

FEBS Open Bio, EarlyView.
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley   +1 more source

Thalamo‐Lesional Connectivity Signatures of Bilateral Tonic–Clonic Seizures in Focal Cortical Dysplasia‐Related Epilepsy

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objectives Focal cortical dysplasia (FCD) is the most common etiology of drug‐resistant epilepsy in children. Focal to bilateral tonic–clonic seizures (FBTCS) mark a high risk of drug‐resistant epilepsy and involve thalamocortical circuitry in their generation and propagation.
Hua Xie, Xiaotong Li, Venkata Sita Priyanka Illapani, Leigh N. Sepeta, Jason Schroeder, Chima O. Oluigbo, Madison M. Berl, William D. Gaillard, Nathan T. Cohen   +8 more
wiley   +1 more source

Fast-Gated 16 × 1 SPAD Array for Non-Line-of-Sight Imaging Applications

Instruments, 2020
In this paper we present a novel single-photon detector specifically designed for Non-Line-Of-Sight (NLOS) imaging applications within the framework of the DARPA REVEAL program.
Marco Renna, Ji Hyun Nam, Mauro Buttafava, Federica Villa, Andreas Velten, Alberto Tosi   +5 more
doaj   +1 more source