Results 121 to 130 of about 7,630,796 (295)

Systematic Activity Maturation of a Single-Domain Antibody with Non-canonical Amino Acids through Chemical Mutagenesis

, 2021
Great advances have been made over the last four decades in therapeutic and diagnostic applications of antibodies. The activity maturation of antibody candidates, however, remains a significant challenge. To address this problem, we present a method that
Bernardes, GJL, Dobson, CM, Aprile, FA, Vendruscolo, M, Lindstedt, PR, Rakoto, R, Sormanni, P, ,   +7 more
core  

Mutant p53R273H disrupts PDPK1 homodimerization and contributes to PDPK1 activation

Molecular Oncology, EarlyView.
How mutant p53R273H drives AKT signaling is unclear. We show that p53R273H, but not wild‐type, directly binds PDPK1 via a mutation‐dependent conformational change. This interaction disrupts inhibitory PDPK1 homodimerization and enhances AKT phosphorylation.
Mei Chee Lim, Alvin Kunyao Guo, Yoko Itahana, Srinivasaraghavan Kannan, Valerie Goh, Mingmin Ong, Terina Yan Wen Tay, Weiying Ye, Sabhashina Radha Krishnan, Jun Nishiyama, Chandra S Verma, Koji Itahana   +11 more
wiley   +1 more source

Targeting the EpCAM‐AXL axis to overcome drug resistance in lung cancer

Molecular Oncology, EarlyView.
Lung cancer cells often evade therapy by hijacking signaling pathways. We reveal that cleaved EpCAM (sEpCAM) stabilizes the oncogenic protein AXL, driving NF‐κB and STAT3‐mediated chemoresistance. This EpCAM‐AXL axis identifies a high‐risk patient subset with poor prognosis.
Alexa Guerrero‐Alba, Mark Shacker, Timothy P. Fleming, Michael A. Smith, Ross M. Bremner, Narendra V. Sankpal   +5 more
wiley   +1 more source

Editorial: Single-Domain Antibodies—Biology, Engineering and Emerging Applications

Frontiers in Immunology, 2018
Kevin A. Henry, C. Roger MacKenzie, C. Roger MacKenzie   +2 more
doaj   +1 more source

Prediction of antigen-responding VHH antibodies by tracking the evolution of antibody along the time course of immunization

Frontiers in Immunology
Antibody maturation is the central function of the adaptive immune response. This process is driven by the repetitive selection of mutations that increase the affinity toward antigens.
Tomonari Matsuda, Yoko Akazawa-Ogawa, Lilian-Kaede Komaba, Norihiko Kiyose, Nobuo Miyazaki, Yusaku Mizuguchi, Tetsuo Fukuta, Yuji Ito, Yoshihisa Hagihara   +8 more
doaj   +1 more source

Engineering IL‐4 resistant proinflammatory human myeloid cells for cancer immunotherapy

Molecular Oncology, EarlyView.
We developed a scalable workflow to generate proinflammatory human myeloid cells. CRISPR/Cas9‐edited CD34+ hematopoietic stem and progenitor cells were expanded and differentiated with M‐CSF. Deletion of STAT6 or STAT6/NFKB1 enhanced macrophage proinflammatory gene expression and cytokine secretion in the presence of IL‐4 while maintaining antibody ...
Theresa Barberi, Alan D. Friedman
wiley   +1 more source

Preparation of a camel-canine chimeric antibody against canine B-cell lymphoma

AMB Express
Canine B-cell lymphoma is a malignant hematologic neoplastic disease, with diffuse large B-cell lymphoma being the predominant subtype. This subtype highly expresses canine CD20 on its surface.
Baohui Li, Fuxiang Bao, Yanlong Liu, Qihuan Zhao, Puchen Li, Bo Wang, Guanyi Ren, Yu Wang   +7 more
doaj   +1 more source

Maximal Domain for Strategy-Proof Rules in Allotment Economies [PDF]

We consider the problem of allocating an amount of a perfectly divisible good among a group of n agents. We study how large a preference domain can be to allow for the existence of strategy-proof, symmetric, and efficient allocation rules when the amount
Hideyuki Mizobuchi, Shigehiro Serizawa
core  

Enhanced antiangiogenic therapy with antibody‐collagen XVIII NC1 domain fusion proteins engineered to exploit matrix remodeling events

, 2006
Antiangiogenic therapy is nowadays one of the most active fields in cancer research. The first strategies, aimed at inhibiting tumor vascularization, included upregulation of endogenous inhibitors and blocking of the signals delivered by angiogenic ...
Álvarez‐Vallina, Luis, Víctor J. Sánchez‐Arévalo Lobo, Francisco J. Blanco, Prieto, Jesús, Blanco, Francisco, Laura Sanz, Compte, Marta, García, Pascal, Jesús Prieto, Cuesta Martínez, Ángel, Ángel M. Cuesta, Sanz, Laura, Sánchez‐Arévalo Lobo, Víctor J., Pascal García, Marta Compte, Luis Álvarez‐Vallina   +15 more
core   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source