Antibody Fragments and Their Purification by Protein L Affinity Chromatography
Antibodies, 2015 Antibodies and related proteins comprise one of the largest and fastest-growing classes of protein pharmaceuticals. A majority of such molecules are monoclonal antibodies; however, many new entities are antibody fragments.
Gustav Rodrigo +2 more
doaj +4 more sources
Protocol to determine antibody affinity and concentration in complex solutions using microfluidic antibody affinity profiling [PDF]
STAR Protocols, 2023 Summary: Conventional methods of measuring affinity are limited by artificial immobilization, large sample volumes, and homogeneous solutions. This protocol describes microfluidic antibody affinity profiling on complex human samples in solution to obtain
Marc Emmenegger +5 more
doaj +2 more sources
DG-Affinity: predicting antigen–antibody affinity with language models from sequences [PDF]
BMC Bioinformatics, 2023 Background Antibody-mediated immune responses play a crucial role in the immune defense of human body. The evolution of bioengineering has led the progress of antibody-derived drugs, showing promising efficacy in cancer and autoimmune disease therapy.
Ye Yuan +4 more
doaj +2 more sources
Microfluidic antibody profiling after repeated SARS-CoV-2 vaccination links antibody affinity and concentration to impaired immunity and variant escape in patients on anti-CD20 therapy [PDF]
Frontiers in Immunology, 2023 BackgroundPatients with autoimmune/inflammatory conditions on anti-CD20 therapies, such as rituximab, have suboptimal humoral responses to vaccination and are vulnerable to poorer clinical outcomes following SARS-CoV-2 infection.
Ashley Priddey +22 more
doaj +2 more sources
Antibody affinity maturation and cross-variant activity following SARS-CoV-2 mRNA vaccination: Impact of prior exposure and sex [PDF]
EBioMedicine, 2021 Background: Limited knowledge exists regarding antibody affinity maturation following mRNA vaccination in naïve vs. COVID-19 recovered individuals and potential sex differences. Methods: We elucidated post-vaccination antibody profiles of 69 naïve and 17
Juanjie Tang +8 more
doaj +2 more sources
Enhancing antibody affinity through experimental sampling of non-deleterious CDR mutations predicted by machine learning [PDF]
Communications Chemistry, 2023 The application of machine learning (ML) models to optimize antibody affinity to an antigen is gaining prominence. Unfortunately, the small and biased nature of the publicly available antibody-antigen interaction datasets makes it challenging to build an
Thomas Clark +11 more
doaj +2 more sources
Optimizing Antibody Affinity and Developability Using a Framework–CDR Shuffling Approach—Application to an Anti-SARS-CoV-2 Antibody [PDF]
Viruses, 2022 The computational methods used for engineering antibodies for clinical development have undergone a transformation from three-dimensional structure-guided approaches to artificial-intelligence- and machine-learning-based approaches that leverage the ...
Ranjani Gopal +5 more
doaj +2 more sources
Antibody affinity versus dengue morphology influences neutralization. [PDF]
PLoS Pathogens, 2021 Different strains within a dengue serotype (DENV1-4) can have smooth, or "bumpy" surface morphologies with different antigenic characteristics at average body temperature (37°C).
Guntur Fibriansah +13 more
doaj +2 more sources
Effect of trifluoroethanol on antibodies binding properties [PDF]
The Ukrainian Biochemical Journal, 2023 The studies on the influence of organic co-solvents on the structure and function of antibodies are of key interest, especially in view of antibodies broad use as recognizing elements in different analytical systems.
S. A. Bobrovnik +2 more
doaj +1 more source
Antibody Affinity Shapes the Choice between Memory and Germinal Center B Cell Fates [PDF]
Cell, 2020 Charlotte Viant +2 more
exaly +2 more sources