Results 11 to 20 of about 526,913 (304)
Studies on antibody affinity in mice
European Journal of Immunology, 1973 AbstractThe plaque inhibition method of measuring the affinity of single antibody‐forming cells (AFC) developed by Andersson has been modified for use with haptens. Using this method, it was possible to make simultaneous determinations of the relative affinities of IgG and IgM plaques during the primary and secondary responses.It was shown that there ...
Huchet, R, Feldmann, M
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Emerging Microbes and Infections, 2022 In the fight against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), monoclonal antibodies (mAbs) serve as key strategies for the rapid prevention and treatment of COVID-19. However, analysis to fully characterize functional SARS-CoV-2 mAbs
Xiaolong Tian +5 more
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Simultaneous Maturation of Single Chain Antibody Stability and Affinity by CHO Cell Display
Bioengineering, 2022 Antibody stability and affinity are two important features of its applications in therapy and diagnosis. Antibody display technologies such as yeast and bacterial displays have been successfully used for improving both affinity and stability.
Ruiqi Luo +6 more
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Antibodies with infinite affinity [PDF]
Proceedings of the National Academy of Sciences, 2001 Here we report an approach to the design and production of antibody/ligand pairs, to achieve functional affinity far greater than avidin/biotin. Using fundamental chemical principles, we have developed antibody/ligand pairs that retain the binding specificity of the antibody, but do not dissociate.
A J, Chmura, M S, Orton, C F, Meares
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AID activity in B cells strongly correlates with polyclonal antibody affinity maturation in-vivo following pandemic 2009-H1N1 vaccination in humans. [PDF]
PLoS Pathogens, 2012 The role of Activation-Induced Cytidine Deaminase (AID) in somatic hypermutation and polyclonal antibody affinity maturation has not been shown for polyclonal responses in humans. We investigated whether AID induction in human B cells following H1N1pdm09
Surender Khurana +3 more
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Affinity improvement of a therapeutic antibody by structure-based computational design: generation of electrostatic interactions in the transition state stabilizes the antibody-antigen complex. [PDF]
PLoS ONE, 2014 The optimization of antibodies is a desirable goal towards the development of better therapeutic strategies. The antibody 11K2 was previously developed as a therapeutic tool for inflammatory diseases, and displays very high affinity (4.6 pM) for its ...
Masato Kiyoshi +8 more
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Non-Affinity Purification of Antibodies
Antibodies, 2023 Currently, purification of antibodies is mainly carried out using a platform technology composed primarily of Protein A chromatography as a capture step, regardless of the scale. However, Protein A chromatography has a number of drawbacks, which are summarized in this review.
Tsutomu Arakawa +7 more
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Effect of β-alanine on humoral immune response in low-dose allergy model
Медицинская иммунология, 2021 At the present time, the efforts of many research groups around the world are aimed at finding new factors triggering the allergic sensitization process linked with IgE synthesis to harmless allergens.
D. B. Chudakov +4 more
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Higher Affinity Antibodies Bind With Lower Hydration and Flexibility in Large Scale Simulations
Frontiers in Immunology, 2022 We have carried out a long-timescale simulation study on crystal structures of nine antibody-antigen pairs, in antigen-bound and antibody-only forms, using molecular dynamics with enhanced sampling and an explicit water model to explore interface ...
Mabel T. Y. Wong +5 more
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Alternative Hapten Design for Zearalenone Immunoreagent Generation
Toxins, 2022 Appropriate hapten design and synthesis have been identified as critical steps to generate high-performance immunoreagents and to develop sensitive and selective immunoanalytical methods.
Antonio Abad-Fuentes +5 more
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