Results 31 to 40 of about 755,061 (295)
Computational Model Reveals a Stochastic Mechanism behind Germinal Center Clonal Bursts
Cells, 2020 Germinal centers (GCs) are specialized compartments within the secondary lymphoid organs where B cells proliferate, differentiate, and mutate their antibody genes in response to the presence of foreign antigens.
Aurélien Pélissier +6 more
doaj +1 more source
Scientific Reports, 2021 Small antibody mimetics that contain high-affinity target-binding peptides can be lower cost alternatives to monoclonal antibodies (mAbs). We have recently developed a method to create small antibody mimetics called FLuctuation-regulated Affinity ...
Kyra See +11 more
doaj +1 more source
PLoS Computational Biology, 2019 Antibodies developed for research and clinical applications may exhibit suboptimal stability, expressibility, or affinity. Existing optimization strategies focus on surface mutations, whereas natural affinity maturation also introduces mutations in the ...
Shira Warszawski +13 more
doaj +1 more source
Facile Affinity Maturation of Antibody Variable Domains Using Natural Diversity Mutagenesis
Frontiers in Immunology, 2017 The identification of mutations that enhance antibody affinity while maintaining high antibody specificity and stability is a time-consuming and laborious process.
Kathryn E. Tiller +6 more
doaj +1 more source
The use of self-assembled receptor layers in immunosensors [PDF]
, 1994 We demonstrate that a self-assembled monolayer consisting of an alkylthiol coupled synthetic peptide adsorbed to a gold substrate results in a sensitive receptor surface for specific recognition of protein molecules. The affinity constant of this binding
Drijfhout, J.W. +3 more
core +3 more sources
Bidirectional optimization of the melting spinning process [PDF]
, 2014 This is the author's accepted manuscript (under the provisional title "Bi-directional optimization of the melting spinning process with an immune-enhanced neural network"). The final published article is available from the link below.
Ding, Y +5 more
core +1 more source
The Immunoglobulins of Cold-Blooded Vertebrates [PDF]
, 2014 Peer reviewedPublisher ...
Chen +22 more
core +2 more sources
Antibody-based affinity cryo-EM grid [PDF]
Methods, 2016 The Affinity Grid technique combines sample purification and cryo-Electron Microscopy (cryo-EM) grid preparation into a single step. Several types of affinity surfaces, including functionalized lipids monolayers, streptavidin 2D crystals, and covalently functionalized carbon surfaces have been reported.
Guimei Yu, Kunpeng Li, Wen Jiang
openaire +2 more sources
Antibody engineering using phage display with a coiled-coil heterodimeric Fv antibody fragment. [PDF]
PLoS ONE, 2011 A Fab-like antibody binding unit, ccFv, in which a pair of heterodimeric coiled-coil domains was fused to V(H) and V(L) for Fv stabilization, was constructed for an anti-VEGF antibody.
Xinwei Wang +3 more
doaj +1 more source
OptMAVEn--a new framework for the de novo design of antibody variable region models targeting specific antigen epitopes. [PDF]
PLoS ONE, 2014 Antibody-based therapeutics provides novel and efficacious treatments for a number of diseases. Traditional experimental approaches for designing therapeutic antibodies rely on raising antibodies against a target antigen in an immunized animal or ...
Tong Li +2 more
doaj +1 more source