Results 31 to 40 of about 526,913 (304)
PLoS Computational Biology, 2019 Antibodies developed for research and clinical applications may exhibit suboptimal stability, expressibility, or affinity. Existing optimization strategies focus on surface mutations, whereas natural affinity maturation also introduces mutations in the ...
Shira Warszawski +13 more
doaj +1 more source
Facile Affinity Maturation of Antibody Variable Domains Using Natural Diversity Mutagenesis
Frontiers in Immunology, 2017 The identification of mutations that enhance antibody affinity while maintaining high antibody specificity and stability is a time-consuming and laborious process.
Kathryn E. Tiller +6 more
doaj +1 more source
A gp41 MPER-specific llama VHH requires a hydrophobic CDR3 for neutralization but not for antigen recognition [PDF]
, 2013 The membrane proximal external region (MPER) of the HIV-1 glycoprotein gp41 is targeted by the broadly neutralizing antibodies 2F5 and 4E10. To date, no immunization regimen in animals or humans has produced HIV-1 neutralizing MPER-specific antibodies ...
Ramos, Alejandra +81 more
core +1 more source
Antibody engineering using phage display with a coiled-coil heterodimeric Fv antibody fragment. [PDF]
PLoS ONE, 2011 A Fab-like antibody binding unit, ccFv, in which a pair of heterodimeric coiled-coil domains was fused to V(H) and V(L) for Fv stabilization, was constructed for an anti-VEGF antibody.
Xinwei Wang +3 more
doaj +1 more source
mAbs, 2020 Complement-dependent cytotoxicity (CDC) is a potent effector mechanism, engaging both innate and adaptive immunity. Although strategies to improve the CDC activity of antibody therapeutics have primarily focused on enhancing the interaction between the ...
Bo Wang +6 more
doaj +1 more source
OptMAVEn--a new framework for the de novo design of antibody variable region models targeting specific antigen epitopes. [PDF]
PLoS ONE, 2014 Antibody-based therapeutics provides novel and efficacious treatments for a number of diseases. Traditional experimental approaches for designing therapeutic antibodies rely on raising antibodies against a target antigen in an immunized animal or ...
Tong Li +2 more
doaj +1 more source
Affinity of Antibodies to MUC1 Antigens
Tumor Biology, 1998 The binding affinity of a monoclonal antibody to its ligand has been often used as a qualitative and quantitative measure for the specificity of the antibody. The aim of this paper was to assess the affinity of binding to recombinant and synthetic MUC1 peptides of 52 monoclonal antibodies as part of the ISOBM TD-4 Workshop.
V, Karanikas +4 more
openaire +2 more sources
Long-lived antibody and B Cell memory responses to the human malaria parasites, Plasmodium falciparum and Plasmodium vivax. [PDF]
, 2010 Antibodies constitute a critical component of the naturally acquired immunity that develops following frequent exposure to malaria. However, specific antibody titres have been reported to decline rapidly in the absence of reinfection, supporting the ...
Thaikla, Kanitta +39 more
core +1 more source
EASINESS: E. coli Assisted Speedy affINity-maturation Evolution SyStem
Frontiers in Immunology, 2021 Antibodies are one of the most important groups of biomolecules for both clinical and basic research and have been developed as potential therapeutics. Affinity is the key feature for biological activity and clinical efficacy of an antibody, especially ...
Hai-nan Zhang +6 more
doaj +1 more source
Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation
FEBS Letters, EarlyView.Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe +3 more
wiley +1 more source