Results 1 to 10 of about 675,780 (316)

In vivo Isolation of Hymenolepis nana and Antibody-binding Sites

Nature, 1962
A RECENT preliminary investigation1 suggests that antibodies are directed against adult specimens of Hymenolepis nana while located in the ileum of the mouse. The present investigation was carried out to substantiate tentative conclusions regarding the specificity of antibody binding sites previously observed. It appeared, from the earlier experiments,
R M, COLEMAN, N M, FOTORNY
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The gemin2-binding site on SMN protein: Accessibility to antibody

Biochemical and Biophysical Research Communications, 2013
Reduced levels of SMN (survival-of-motor-neurons) protein are the cause of spinal muscular atrophy, an inherited disorder characterised by loss of motor neurons in early childhood. SMN associates with more than eight other proteins to form an RNA-binding complex involved in assembly of the spliceosome.
Le Thanh, Lam, Heidi R, Fuller, Glenn E, Morris   +2 more
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Hapten conformation in the combining site of antibodies that bind phenylphosphocholine

Biochemistry, 1992
We have shown previously that anti-phenylphosphocholine antibodies elicited by phosphocholine-keyhole limpet hemocyanin can be divided into two populations according to their ability to recognize the two hapten analogues p-nitrophenylphosphocholine (NPPC) and p-nitrophenyl 3,3-dimethylbutyl phosphate (NPDBP).
U, Bruderer, D H, Peyton, E, Barbar, J H, Fellman, M B, Rittenberg   +4 more
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Ouantitation of granulocyte antibodies in sera and determination of their binding sites

British Journal of Haematology, 1992
Summary An enzyme immunoassay using eluates was developed for the quantitation of granulocyte antibodies in sera. Incubation of donor granulocytes with sera containing granulocyte alloantibodies (NA1, NA2, NB1, 5b. HLA) or autoantibodies resulted in a significantly higher amount of immunoglobulin G (IgG) per cell than did incubation with control sera ...
J, Bux, M, Sohn, R, Hachmann, C, Mueller-Eckhardt   +3 more
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Ligand Binding to Anti-Fluorescyl Antibodies: Stability of the Antigen Binding Site

Biochemistry, 1994
The problem of protein stability is addressed with spectroscopic studies of equilibrium and kinetic properties of the binding of fluorescein to high-affinity monoclonal anti-fluorescyl antibodies (Mab 4-4-20), Fab fragments, and single-chain antibodies (SCA). SCA molecules contain only the variable domains of the antibody and an amino acid linker.
Müller, J. D., Nienhaus, G. U., Tetin, S. Y., Voss, E. W.   +3 more
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Binding Sites of Monoclonal Anti-Carbohydrate Antibodies

1988
This chapter presents an overview of monoclonal antibodies (mAbs) directed to carbohydrates in mammalian systems with well-characterized specificities, together with some of the information obtained from their use. Because of the rapidly increasing number of this kind of reagent not all available mAbs are included.
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Characterization of an anti-digoxin antibody binding site by site-directed in vitro mutagenesis

Molecular Immunology, 1993
In vitro mutagenesis and immunoglobulin gene transfection were used to investigate the binding site of a monoclonal antibody, 2610, that binds to digoxin, a cardiac glycoside. A computer model was generated in order to select sites in the complementarity determining regions (CDR) that would participate in binding.
R I, Near, M, Mudgett-Hunter, J, Novotny, R, Bruccoleri, S C, Ng   +4 more
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Conjugation of a Reactive Thiol at the Nucleotide Binding Site for Site-Specific Antibody Functionalization

Bioconjugate Chemistry, 2014
Described here is a UV photo-cross-linking method that utilizes the NBS (nucleotide binding site) for site-specific covalent functionalization of antibodies with reactive thiol moieties (UV-NBS(Thiol)), while preserving antibody activity. By synthesizing an indole-3-butyric acid (IBA) conjugated version of cysteine we site-specifically photo-cross ...
Nathan J, Alves, Nur, Mustafaoglu, Basar, Bilgicer   +2 more
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Ca2+-binding site of carp parvalbumin recognized by monoclonal antibody

Cell Calcium, 1990
Monoclonal antibody 235 which was used for immunohistochemical staining of parvalbumin in tissue sections partially protects Lys-54 of carp muscle parvalbumin from reaction with acetic anhydride in the parvalbumin-antibody complex. Lys-54 is located in the CD-loop of parvalbumin and is flanked by the Ca2(+)-ligands Asp-53 and Ser-55 of the Ca2(+)-site ...
Tinner R, Oertle M, Heizmann CW, Bosshard HR   +3 more
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Antigen-binding sites dominate the surface properties of IgG antibodies

Molecular Immunology, 1995
A new technique, liquid-liquid partition chromatography in an aqueous polyethylene glycol-dextran two-phase system, was used to detect differences in surface properties of antibodies with different antigen-binding sites. Employing well-characterized monoclonal IgG antibodies and Fab and Fc fragments thereof as well as chimeric IgG antibodies we found a
C, Wingren, U B, Hansson, C G, Magnusson, M, Ohlin   +3 more
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