Results 41 to 50 of about 526,913 (304)

Epitope-Based Specific Antibody Modification

, 2023
Modified antibodies play essential roles in various fields, such as analysis, diagnosis, and therapy, which require modified antibodies with optimal physical and biological properties.
Saki, Horie, Kenji, Mishiro, Mio, Nishino, Inori, Domae, Mitsuo, Wakasugi, Tsukasa, Matsunaga, Munetaka, Kunishima   +6 more
core   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

Maximizing activity and selectivity of antibody-mediated effector functions using antibody mixtures

mAbs
Fc-mediated effector functions are key for conferring potent antibody-mediated killing of cancer cells. However, it is difficult to achieve highly selective targeting of cancer cells while minimizing toxicity on healthy tissue because of the expression ...
Tiexin Wang, Alec A. Desai, Greg M. Thurber, Peter M. Tessier   +3 more
doaj   +1 more source

Engineering SARS-CoV-2 neutralizing antibodies for increased potency and reduced viral escape pathways

iScience, 2022
Summary: The rapid spread of SARS-CoV-2 variants poses a constant threat of escape from monoclonal antibody and vaccine countermeasures. Mutations in the ACE2 receptor binding site on the surface S protein have been shown to disrupt antibody binding and ...
Fangzhu Zhao, Celina Keating, Gabriel Ozorowski, Namir Shaabani, Irene M. Francino-Urdaniz, Shawn Barman, Oliver Limbo, Alison Burns, Panpan Zhou, Michael J. Ricciardi, Jordan Woehl, Quoc Tran, Hannah L. Turner, Linghang Peng, Deli Huang, David Nemazee, Raiees Andrabi, Devin Sok, John R. Teijaro, Timothy A. Whitehead, Andrew B. Ward, Dennis R. Burton, Joseph G. Jardine   +22 more
doaj   +1 more source

Generation and application of recombinant antibody fragments for prostate cancer detection. [PDF]

, 2010
Prostate cancer (PCa) is the most prevalent adenocarcinoma and the second highest cause of cancer-related deaths in men. Early diagnosis is required to identify the development of PCa to reduce the risk of the disease metastasising to different regions ...
Hayes, Conor
core  

Stable expression of a recombinant human antinucleosome antibody to investigate relationships between antibody sequence, binding properties, and pathogenicity

, 2005
When purified under rigorous conditions, some murine anti-double-stranded-DNA (anti-dsDNA) antibodies actually bind chromatin rather than dsDNA. This suggests that they may actually be antinucleosome antibodies that only appear to bind dsDNA when they ...
Lambrianides, A, Rahman, A, Anisur Rahman, Isenberg, DA, Mason, LJ, David S Latchman, Lesley J Mason, Manson, JJ, Latchman, DS, Jessica J Manson, David A Isenberg, Joanna D Haley, Haley, JD, Anastasia Lambrianides   +13 more
core   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Physiological Considerations for Modeling in vivo Antibody-Target Interactions

Frontiers in Pharmacology, 2022
The number of therapeutic antibodies in development pipelines is increasing rapidly. Despite superior success rates relative to small molecules, therapeutic antibodies still face many unique development challenges. There is often a translational gap from
Tyler Dunlap, Yanguang Cao, Yanguang Cao
doaj   +1 more source

Molecular Modeling and Affinity Determination of scFv Antibody: Proper Linker Peptide Enhances Its Activity

, 2010
One of existing strategies to engineer active antibody is to link VH and VL domains via a linker peptide. How the composition, length, and conformation of the linker affect antibody activity, however, remains poorly understood.
Li Y, Long M(龙勉), 龙勉, Gu X, Huang Y, Sun YX, Feng JN, Shen BF, Geng SS, Jia XL(贾潇凌), Wang Y   +10 more
core   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source