Results 21 to 30 of about 755,061 (295)

Higher Affinity Antibodies Bind With Lower Hydration and Flexibility in Large Scale Simulations

Frontiers in Immunology, 2022
We have carried out a long-timescale simulation study on crystal structures of nine antibody-antigen pairs, in antigen-bound and antibody-only forms, using molecular dynamics with enhanced sampling and an explicit water model to explore interface ...
Mabel T. Y. Wong, Sebastian Kelm, Xiaofeng Liu, Richard D. Taylor, Terry Baker, Jonathan W. Essex   +5 more
doaj   +1 more source

Affinity of anti-spike antibodies in SARS-CoV-2 patient plasma and its effect on COVID-19 antibody assays

EBioMedicine, 2022
Summary: Background: Measuring anti-spike protein antibodies in human plasma or serum is commonly used to determine prior exposure to SARS-CoV-2 infection and to assess the anti-viral protection capacity.
Patrick J. Macdonald, Qiaoqiao Ruan, Jessica L. Grieshaber, Kerry M. Swift, Russell E. Taylor, John C. Prostko, Sergey Y. Tetin   +6 more
doaj   +1 more source

Enhancement of the neutralization ability resulting from a single amino acid change in the light chain of a chimeric antibody against SARS-CoV-2

Shanghai Jiaotong Daxue xuebao. Yixue ban, 2023
Objective·To screen the human-mouse chimeric antibodies that neutralize the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and analyse the key amino acid site changes in their light chain.Methods·Antibody heavy chain gene-humanized mice ...
YIN Zi, LIAN Chaoyang, GAO Bo, TIAN Ying, HAO Qian, YEAP Lengsiew   +5 more
doaj   +1 more source

Effects of antibodies against tubulin on the movement of reactivated sea urchin sperm flagella [PDF]

, 1980
Antibodies binding to sea urchin flagellar outer-doublet tubulin have been isolated from rabbit sera by tubulin-affinity chromatography employing electrophoretically purified tubulin as the immobilized substrate.
Asai, David J., Brokaw, Charles J.
core   +1 more source

IgG Fc Affinity Ligands and Their Applications in Antibody-Involved Drug Delivery: A Brief Review

Pharmaceutics, 2023
Antibodies are not only an important class of biotherapeutic drugs, but also are targeting moieties for achieving active targeting drug delivery. Meanwhile, the rapidly increasing application of antibodies and Fc-fusion proteins has inspired the emerging
Chang Yang, Bing He, Hua Zhang, Xueqing Wang, Qiang Zhang, Wenbing Dai   +5 more
doaj   +1 more source

Generation and in vivo characterization of a chimeric αvβ5-targeting antibody 14C5 and its derivatives [PDF]

, 2013
Background: Previous studies showed that radiolabeled murine monoclonal antibody (mAb) 14C5 and its Fab and F(ab')2 fragments, targeting αvβ5 integrin, have promising properties for diagnostic and therapeutic applications in cancer.
De Vos, Filip, Dumolyn, Caroline, Haustraete, Jurgen, Moerman, Lieselotte, Neyt, Sara, Schoonooghe, Steve   +5 more
core   +2 more sources

Surface Plasmon Resonance kinetic analysis of the interaction between G-quadruplex nucleic acids and an anti-G-quadruplex monoclonal antibody [PDF]

, 2018
Background G-quadruplexes (G4s) are nucleic acids secondary structures formed in guanine-rich sequences. Anti-G4 antibodies represent a tool for the direct investigation of G4s in cells.
Lago, Sara, Nadai, Matteo, Richter, Sara N, Rossetto, Monica   +3 more
core   +1 more source

Structure Based Affinity Maturation and Characterizing of SARS-CoV Antibody CR3022 against SARS-CoV-2 by Computational and Experimental Approaches

Viruses, 2022
The COVID-19 epidemic is raging around the world. Neutralizing antibodies are powerful tools for the prevention and treatment of SARS-CoV-2 infection. Antibody CR3022, a SARS-CoV neutralizing antibody, was found to cross-react with SARS-CoV-2, but its ...
Wei Yu, Nan Zhong, Xin Li, Jiayi Ren, Yueming Wang, Chengming Li, Gui Yao, Rui Zhu, Xiaoli Wang, Zhenxing Jia, Changwen Wu, Rongfeng Chen, Weihong Zheng, Huaxin Liao, Xiaomin Wu, Xiaohui Yuan   +15 more
doaj   +1 more source

Endogenous mammalian lectin localized extracellularly in lung elastic fibers. [PDF]

, 1984
An affinity-purified antibody preparation raised against a beta-galactoside-binding lectin from bovine lung was used to localize a similar lectin in rat lung by immunofluorescence and by electron microscopy after on-grid staining visualized with ...
Barondes, SH, Cerra, RF, Haywood-Reid, PL   +2 more
core   +3 more sources

Antibody Fragments and Their Purification by Protein L Affinity Chromatography

Antibodies, 2015
Antibodies and related proteins comprise one of the largest and fastest-growing classes of protein pharmaceuticals. A majority of such molecules are monoclonal antibodies; however, many new entities are antibody fragments.
Gustav Rodrigo, Mats Gruvegård, James M. Van Alstine   +2 more
doaj   +1 more source