Results 101 to 110 of about 71,132 (292)

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Protocol for effective surface passivation for single-molecule studies of chromatin and topoisomerase II

STAR Protocols
Summary: For single-molecule studies requiring surface anchoring of biomolecules, poorly passivated surfaces can result in alterations of biomolecule structure and function that lead to artifacts.
Tung T. Le, Xiang Gao, Seong Ha Park, Jaeyoon Lee, James T. Inman, Michelle D. Wang   +5 more
doaj   +1 more source

Versatile Control System for Automated Single-Molecule Optical Tweezers Investigations

, 2010
We present a versatile control system to automate single-molecule biophysics experiments. This method combines low-level controls into various functional, user-configurable modules, which can be scripted in a domain-specific instruction language.
Steven J. Koch, Richard C. Yeh
core  

Single-molecule protein dynamics during DNA replication

, 2022
Observing the processes of life that occur in all cellular organisms at the level of single molecules has allowed a deeper understanding of the dynamic processes taking place in complex biological systems.
Gurleen Kaur (11041128)
core  

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Protocol for sample preparation and single-molecule optical tweezers study of nanodisc-embedded ABC transporter OpuA

STAR Protocols
Summary: OpuA is an osmoregulatory ATP-binding cassette transporter that undergoes different conformations upon varying salt concentrations and ligand conditions.
Lyan van der Sleen, Marco van den Noort, Laura-Marie Silbermann, Bert Poolman, Katarzyna Tych   +4 more
doaj   +1 more source

Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1

FEBS Open Bio, EarlyView.
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay, Junjie Xu, Josep Rizo   +2 more
wiley   +1 more source

A protocol for single molecule imaging and tracking of processive myosin motors

MethodsX, 2019
Myosin is a large family of actin-based molecular motors, which includes efficient intracellular transporters that move cargoes and material essential for cell’s life.
Lucia Gardini, Claudia Arbore, Marco Capitanio, Francesco Saverio Pavone   +3 more
doaj   +1 more source

DNA replication at the single-molecule level [PDF]

, 2014
A cell can be thought of as a highly sophisticated micro factory: in a pool of billions of molecules – metabolites, structural proteins, enzymes, oligonucleotides – multi-subunit complexes assemble to perform a large number of basic cellular tasks, such ...
Oijen, A.M. van, Oijen, A.M. van,, Stratmann, S.A.,, Stratmann, S.A.   +3 more
core   +1 more source

Unravelling How Single-Stranded DNA Binding Protein Coordinates DNA Metabolism Using Single-Molecule Approaches

, 2023
Single-stranded DNA-binding proteins (SSBs) play vital roles in DNA metabolism. Proteins of the SSB family exclusively and transiently bind to ssDNA, preventing the DNA double helix from re-annealing and maintaining genome integrity.
Matthew T. J. Halma, Wuite, Gijs J.L.; id_orcid, Longfu Xu, Halma, Matthew T.J., Wuite, Gijs J.L., Xu, Longfu, Gijs J. L. Wuite   +6 more
core   +1 more source