Results 131 to 140 of about 71,132 (292)

Observation of long-range tertiary interactions during ligand binding by the TPP riboswitch aptamer

eLife, 2015
The thiamine pyrophosphate (TPP) riboswitch is a cis-regulatory element in mRNA that modifies gene expression in response to TPP concentration. Its specificity is dependent upon conformational changes that take place within its aptamer domain.
Van K Duesterberg, Irena T Fischer-Hwang, Christian F Perez, Daniel W Hogan, Steven M Block   +4 more
doaj   +1 more source

Analysis methods for single molecule fluorescence spectroscopy

, 2012
This thesis describes signal analysis methods for single-molecule fluorescence data. The primary factor motivating method development is the need to distinguish single-molecule FRET fluctuations due to conformational dynamics from fluctuations due to ...
Gryte, Kristofer
core   +1 more source

Swelling‐Programmed Topographical Guidance for Dynamic Spheroid Self‐Assembly via a Mechanochemical Hydrogel Niche

Advanced Functional Materials, EarlyView.
A swelling‐programmed micropatterned hydrogel guides adherent cells through a controlled transition from cell–matrix anchoring to cadherin‐mediated cell–cell compaction, enabling rapid assembly of high‐viability spheroids with defined size and morphology.
Han Gyeol Nam, Seon Yeong Chae, Young Woo Kwon, Hye Jin Heo, Hye Rin Lee, Ji Hyeok Lee, Dong‐Wook Han, Yun Hak Kim, Suck Won Hong   +8 more
wiley   +1 more source

Improving certainty in single molecule imaging

, 2017
The spatiotemporal organization of biological entities and the complex network of interactions they sponsor has proven challenging to visualize. Modern biophysics has brought a wealth of techniques for probing cellular structure and dynamics of which ...
Ana J García-Sáez, John SH Danial, Danial, J., García-Sáez, A. ; https://orcid.org/   +3 more
core   +1 more source

Intrinsic Photoactive Star ZnPc–Poly(glutamate) Nanoplatforms for Multimodal Glioblastoma Therapy and Brain‐Targeted Delivery

Advanced Functional Materials, EarlyView.
An intrinsic photoactive star‐shaped zinc phtalocyanine‐poly(L‐glutamic acid) (ZnPc‐PGA) nanoplatform for multimodal glioblastoma (GBM) therapy and brain‐targeted elivery. A ZnPc‐PGA‐based multifunctional theranostic nanocarrier platform enables image‐guided, multimodal GBM therapy. ZnPc‐PGA nanocarriers support the integration of fluorescence imaging,
Amina Benaicha‐Fernández, Teodora Ranđelović, Ana Armiñán, Víctor Sebastián, Manuel Fuentes, Cristián Huck‐Iriart, Esther Masiá, Paula Carrascosa‐Marco, Pablo Juanes‐Velasco, Snežana Đorđević, Clara Bayona, Ignacio Ochoa, Maria Medel, Inmaculada Conejos‐Sánchez, María J. Vicent   +14 more
wiley   +1 more source

Single-molecule study of the DNA helicase regulating genomic stability

, 2011
A DNA helicase, PcrA is an essential protein in gram-positive bacteria. PcrA utilizes its helicase activity and translocation activity to regulate various cellular functions including plasmid replication and counteracting deleterious recombination ...
Park, Jeehae
core  

Atomic Force Microscopy of Protein–Protein Interactions

, 2009
Since its invention in 1986, the atomic force microscope (AFM) has emerged as a flexible and powerful tool for exploring a variety of biological processes, including cell adhesion, protein folding, and protein–protein interactions. This review focuses on
Xiaohui Zhang, Zhang, Xiaohui, Amy J. Xu, Felix Rico, Xu, Amy J, Moy, Vincent T, Rico, Felix, Vincent T. Moy   +7 more
core   +1 more source

Double‐Interpenetrating Composite Bioink for Multi‐Component 3D Bioprinting and Biofabrication

Advanced Functional Materials, EarlyView.
Marine‐polysaccharides‐derived double interpenetrating network hydrogel‐based bioink, combining the attributes of physical and ionic crosslinking, enables room‐temperature bioprinting of mechanically defined structures that can be handled and are cytocompatible.
Fabian Tribukait‐Riemenschneider, V. Prasad Shastri   +1 more
wiley   +1 more source

Single Molecule Conformational Memory Extraction: P5ab RNA Hairpin

, 2014
Extracting kinetic models from single molecule data is an important route to mechanistic insight in biophysics, chemistry, and biology. Data collected from force spectroscopy can probe discrete hops of a single molecule between different conformational ...
Elms, P, Maccallum, JL, Marqusee, Susan, Pressé, S, Lee, J, Steve Pressé, Justin L. MacCallum, Dill, Ken, Jack Peterson, Peterson, J, Peterson, Jack, Phillip Elms, Bustamante, Carlos, Julian Lee, Pressé, Steve, Carlos Bustamante, Lee, Julian, Ken Dill, MacCallum, Justin L., Susan Marqusee, Elms, Phillip, Bustamante, C, Marqusee, S   +22 more
core   +1 more source

Coagulative Granular Hydrogels with an Enzyme Catalyzed Fibrin Network for Endogenous Tissue Regeneration

Advanced Healthcare Materials, EarlyView.
Coagulative granular hydrogels are composed of packed thrombin‐functionalized microgels that catalyze the conversion of fibrinogen into a secondary fibrin network, filling the interstitial voids. This bio‐inspired approach stabilizes the biomaterial to match the robustness of bulk hydrogels without compromising injectability, mimicking the initial ...
Zhipeng Deng, Camila B. Tovani, Simona Bianco, Gianni Comandini, Aya Elghajiji, Mina Aleemardani, Roxana Moscalu, Jeremie Zappia, Bianca Fernandes, Olivia Annett, Chrissy L. Hammond, Jason Wong, Dave J. Adams, Fabrizio Scarpa, Michael R. Whitehouse, Annela M. Seddon, James P. K. Armstrong   +16 more
wiley   +1 more source