Results 151 to 160 of about 535,239 (304)

microRNA-directed phasing during trans-acting siRNA biogenesis in plants.

Cell, 2005
E. Allen, Zhixin Xie, Adam M. Gustafson, J. C. Carrington   +3 more
semanticscholar   +1 more source

Genome‐Wide In Vivo RNAi Screening Identifies HOXD4 as a Tumor Metastasis Suppressor in Colorectal Cancer

Advanced Science, EarlyView.
Metastasis remains a major challenge in colorectal cancer. Using an in vivo shRNA screening system, this study identifies Homeobox D4 as a key metastasis suppressor. Reduced Homeobox D4 expression is associated with aggressive tumor features. Functional and mechanistic analyses show that it inhibits epithelial‐mesenchymal transition by repressing ...
Zhi‐hua Ye, Wen‐jing Luo, Lu Li, Wen‐di Shuai, Ling Zhou, You‐fa Duan, Xue Chen, Jun‐kai Zhang, Wenlin Huang, Ran‐yi Liu   +9 more
wiley   +1 more source

siRNA-based knockdown of CLU results in decreased VWF and CLU protein expression but no change in VWF mRNA content.

qPCR analysis of CLU expression demonstrates significant decrease in CLU expression after CLU siRNA knockdown (2A). As expected, VWF siRNA knockdown has no effect on CLU expression (2A).
Allaura A. Cox (17980027), Alice Liu (4814415), Christopher J. Ng (14290358)   +2 more
core   +1 more source

FGF13 Deficiency Ameliorates Paclitaxel‐Induced Neuropathic Pain by Inhibiting VASH1‐Mediated Microtubule Detyrosination to Promote Mitophagy

Advanced Science, EarlyView.
FGF13 is upregulated in DRG neurons of PIPNP model mice. DRG neuron‐specific knockout of FGF13 ameliorates PIPNP symptoms. Mechanistically, FGF13 potentiates microtubule detyrosination by promoting VASH1 binding to microtubules. FGF13 knockout suppresses VASH1‐mediated microtubule detyrosination and promotes α‐tubulin tyrosination.
Yiming Dong, Yidan Wang, Simeng Lv, Zishan Dong, Kaixi Zhi, Xiuhua Guo, Xuyan Li, Ruoxi Yu, Yiyi Zhang, Siyuan Cheng, Chuan Wang   +10 more
wiley   +1 more source

siRNA-mediated knockdown of AP-5 reduces plasma membrane localization and increases punctate distribution of HIV-2 Gag.

, 2016
(A) HeLa cells were transfected with 25 nM AP-5 siRNA or negative control siRNA (-ve), or mock transfected (none). Cells were harvested 42 hours post-transfection, from which RNA was isolated and RT-qPCR carried out to detect AP-5μ mRNA.
Emma C. Anderson (533572), Justine E. Alford (533570), Gemma L. Watkins (3156042), Michela Marongiu (3156045)   +3 more
core   +1 more source

Single‐Cell Dissection of Therapy‐Induced Remodeling Uncovers a Fibroblast‐Driven Immunosuppressive Niche and Targetable Vulnerabilities in Lethal Prostate Cancer

Advanced Science, EarlyView.
Single‐cell longitudinal profiling reveals that androgen‐deprivation therapy induces a DPT+ fibroblast‐complement axis that suppresses macrophage inflammation and drives CD8+ T cell exhaustion in prostate cancer. Concurrently, resistant epithelial subpopulations persist and engage TSPAN1‐ and NRXN1‐mediated programs promoting CRPC and neuroendocrine ...
Yang Chen, Dandan Dong, Jinling Liao, Naikai Liao, Mengqi Liu, Qin Zhang, Siting Chen, Qi Liu, Ying Lu, Tianyu Li, Chunlin Zou, Qiong Song, Qiuyan Wang, Jian Liang, Qiji Xie, Chengyang Li, Mengyun Wang, Jiwen Cheng, Zengnan Mo, Gong‐Hong Wei   +19 more
wiley   +1 more source

siRNA [PDF]

Chemistry & Biology, 2002
Ramaswamy, Gopalakrishna, Slack, Frank J
openaire   +1 more source

siRNA-mediated knockdown of AP-5 affects HIV-2 but not HIV-1 Gag.

, 2016
HeLa cells were co-transfected with HIV-1 (A, B) or HIV-2 (C, D) proviral plasmids and no siRNA (none), siRNA against AP-5μ or a negative control siRNA (-ve). 42 hours post-transfection, cell lysates and viral supernatants were harvested. Cell-associated
Emma C. Anderson (533572), Justine E. Alford (533570), Gemma L. Watkins (3156042), Michela Marongiu (3156045)   +3 more
core   +1 more source

TRAIP Mediates Alcohol‐Induced Liver Injury through Regulating β‐catenin Ubiquitin Degradation via Direct Interaction

Advanced Science, EarlyView.
This study reveals that NF‐κB1‐driven TRAIP upregulation in ALD correlates with disease severity. Mechanistically, TRAIP directly binds β‐catenin via its CC domain and promotes its K48‐linked ubiquitination and degradation, which is independent of the GSK3β/β‐TrCP pathway.
Zhan Wu, Mingjiang Liu, Lijuan Liao, Zhaohong Mo, Wenli Xu, Linfeng Mao, Yubing Chen, Qiuli Xie, Ming Yao, Shilian Chen, Stephen Tomlinson, Sebastian Mueller, Songqing He, Guandou Yuan   +13 more
wiley   +1 more source

siRNA-mediated knockdown of RIPK3 reduces expression in human macrophages.

Immunoblot analysis of phosphorylated receptor-interacting protein kinase 3 (pRIPK3) and total RIPK3 (tRIPK3) expression in human macrophages treated with non-targeting siRNA (siControl) or human-specific RIPK3 siRNA (siRIPK3), followed by treatment with
Sk Mohiuddin Choudhury (18065118), Roman Sarkar (10583678), Rajendra Karki (460028), Thirumala-Devi Kanneganti (62745)   +3 more
core   +1 more source