Results 261 to 270 of about 411,726 (313)
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2021
Exosomes are membrane-bound vesicles (40-100 nm) of endocytic origin released by numerous cell types that act as natural carriers of mRNA, microRNA, and proteins between cells. We developed a new system that uses intravenous injection of modified exosomes for siRNA delivery into the brain.
María, Izco, Lydia, Alvarez-Erviti
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Exosomes are membrane-bound vesicles (40-100 nm) of endocytic origin released by numerous cell types that act as natural carriers of mRNA, microRNA, and proteins between cells. We developed a new system that uses intravenous injection of modified exosomes for siRNA delivery into the brain.
María, Izco, Lydia, Alvarez-Erviti
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Chitosan/siRNA Nanoparticles Encapsulated in PLGA Nanofibers for siRNA Delivery
ACS Nano, 2012Composite nanofibers of biodegradable poly(D,L-lactic-co-glycolic acid) (PLGA) encapsulating chitosan/siRNA nanoparticles (NPs) were prepared by electrospinning. Acidic/alkaline hydrolysis and a bulk/surface degradation mechanism were investigated in order to achieve an optimized release profile for prolonged and efficient gene silencing.
Chen, Menglin; id_orcid 0000-0002-2721-651X +7 more
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Oligonucleotides, 2009
Canonical small interfering RNA (siRNA) duplexes are potent activators of the mammalian innate immune system. The induction of innate immunity by siRNA is dependent on siRNA structure and sequence, method of delivery, and cell type. Synthetic siRNA in delivery vehicles that facilitate cellular uptake can induce high levels of inflammatory cytokines and
Marjorie, Robbins +2 more
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Canonical small interfering RNA (siRNA) duplexes are potent activators of the mammalian innate immune system. The induction of innate immunity by siRNA is dependent on siRNA structure and sequence, method of delivery, and cell type. Synthetic siRNA in delivery vehicles that facilitate cellular uptake can induce high levels of inflammatory cytokines and
Marjorie, Robbins +2 more
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Imaging of Electrotransferred siRNA
2016SiRNA delivery to the cytoplasm can be obtained through the application of calibrated electric field pulses to a mixture of cells and oligonucleotides. To investigate the uptake pathway, time lapse confocal fluorescence microscopy provides a direct visualization of the transfer.
Muriel, Golzio, Justin, Teissié
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siRNA and Cancer Immunotherapy
Immunotherapy, 2012Immunotherapeutic approaches have been gaining attention in the field of cancer treatment because of their possible ability to eradicate cancer cells as well as metastases by recruiting the host immune system. On the other hand, RNA-based therapeutics with the ability to silence expression of specific targets are currently under clinical investigation ...
Soudeh, Ghafouri-Fard +1 more
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Cold Spring Harbor Protocols, 2019
This protocol describes how to anneal synthetic sense and antisense siRNAs to form siRNA duplexes, as well as the analysis of siRNA duplexes using nondenaturing polyacrylamide gel electrophoresis.
Chengjian, Li, Phillip D, Zamore
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This protocol describes how to anneal synthetic sense and antisense siRNAs to form siRNA duplexes, as well as the analysis of siRNA duplexes using nondenaturing polyacrylamide gel electrophoresis.
Chengjian, Li, Phillip D, Zamore
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Drug Discovery Today: Technologies, 2010
siRNA technology has not only greatly facilitated functional characterization of new genes, but also presented tremendous potential for becoming the next generation of blockbuster drugs. The technology has penetrated life science research in an extremely fast speed, thus providing a huge knowledge base for siRNA drug development.
Xiaoxia Wang +3 more
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siRNA technology has not only greatly facilitated functional characterization of new genes, but also presented tremendous potential for becoming the next generation of blockbuster drugs. The technology has penetrated life science research in an extremely fast speed, thus providing a huge knowledge base for siRNA drug development.
Xiaoxia Wang +3 more
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Nature Methods, 2006
A new study addresses how to design highly specific small interfering RNAs (siRNAs) capable of distinguishing between similar variants of the same gene.
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A new study addresses how to design highly specific small interfering RNAs (siRNAs) capable of distinguishing between similar variants of the same gene.
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Targeting Oncogenes with siRNAs
2008Current cancer chemotherapies heavily rely on the unspecific inhibition of proliferating cells. This lack of tumour cell specificity results in severe toxic side effects and may only hardly affect quiescent cancer stem cells consequently leading to relapse. Since oncogenes are exclusively expressed in malignant and pre-malignant cells, they may provide
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Annealing siRNAs to Produce siRNA Duplexes
Cold Spring Harbor Protocols, 2006openaire +2 more sources