Results 31 to 40 of about 10,065,751 (268)
BioTechniques, 2011 Site-specific recombination is a powerful biotechnological tool for genome engineering. We previously reported two novel site-specific recombination systems, VCre/VloxP and SCre/SloxP, that do not cross-react with Cre/loxP and Flp/FRT in culture cells ...
Sachiko Minorikawa, Manabu Nakayama
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Novel Heterotypic Rox Sites for Combinatorial Dre Recombination Strategies
G3: Genes, Genomes, Genetics, 2016 Site-specific recombinases (SSRs) such as Cre are widely used in gene targeting and genetic approaches for cell labeling and manipulation. They mediate DNA strand exchange between two DNA molecules at dedicated recognition sites. Precise understanding of
Katherine Chuang +3 more
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A method for producing transgenic cells using a multi-integrase system on a human artificial chromosome vector. [PDF]
PLoS ONE, 2011 The production of cells capable of expressing gene(s) of interest is important for a variety of applications in biomedicine and biotechnology, including gene therapy and animal transgenesis.
Shigeyuki Yamaguchi +5 more
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An att site-based recombination reporter system for genome engineering and synthetic DNA assembly
BMC Biotechnology, 2017 Background Direct manipulation of the genome is a widespread technique for genetic studies and synthetic biology applications. The tyrosine and serine site-specific recombination systems of bacteriophages HK022 and ΦC31 are widely used for stable ...
Michael J. Bland +3 more
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Development of a novel in vitro assay for the evaluation of integron DNA integrase activity
Biotechnology & Biotechnological Equipment, 2016 Integrons play an important role in multidrug resistance. The integron platform codes for integrase (intI) that is required for gene cassette integration through site-specific recombination.
Fatemeh Tohidi +6 more
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Virulence, 2020 The proliferation of antibiotic resistance has its origins in horizontal gene transfer. The class 1 integrons mediate gene transfer by assimilating antibiotic-resistance genes through site-specific recombination.
Jun Zhang +8 more
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Xer site‐specific recombination in vitro. [PDF]
The EMBO Journal, 1995 Two related recombinases, XerC and XerD, belonging to the lambda integrase family of enzymes, are required for Xer site-specific recombination in vivo. In order to understand the roles of these proteins in the overall reaction mechanism, an in vitro recombination system using a synthetic Holliday junction-containing substrate has been developed ...
L K, Arciszewska, D J, Sherratt
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dif Site-Specific Recombination and Chromosome Segregation [PDF]
, 1994 Linear chromosomes, for example those of eukaryotes, have evolved mechanisms to ensure that chromosome ends are protected from exonucleolytic attack and can be replicated completely. Circular chromosomes are not subject to these problems.
Leslie, Nicholas Reid
core +7 more sources
The catalytic residues of Tn3 resolvase [PDF]
, 2009 To characterize the residues that participate in the catalysis of DNA cleavage and rejoining by the site-specific recombinase Tn3 resolvase, we mutated conserved polar or charged residues in the catalytic domain of an activated resolvase variant.
Olorunniji, F.J. +5 more
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Site-specific recombination in plane view [PDF]
Structure, 1997 Biochemists have worked long and hard on each reaction component and chemical step to reach the point of asking the question as to how protein and DNA molecules are arranged and rearranged in the process of site-specific recombination. The structures of several lambda integrase family members published recently have answered many of the questions about
Yang, Wei, Mizuuchi, Kiyoshi
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