Results 31 to 40 of about 283,041 (288)
BioTechniques, 2011 Site-specific recombination is a powerful biotechnological tool for genome engineering. We previously reported two novel site-specific recombination systems, VCre/VloxP and SCre/SloxP, that do not cross-react with Cre/loxP and Flp/FRT in culture cells ...
Sachiko Minorikawa, Manabu Nakayama
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The catalytic residues of Tn3 resolvase [PDF]
, 2009 To characterize the residues that participate in the catalysis of DNA cleavage and rejoining by the site-specific recombinase Tn3 resolvase, we mutated conserved polar or charged residues in the catalytic domain of an activated resolvase variant.
Olorunniji, F.J. +5 more
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Novel Heterotypic Rox Sites for Combinatorial Dre Recombination Strategies
G3: Genes, Genomes, Genetics, 2016 Site-specific recombinases (SSRs) such as Cre are widely used in gene targeting and genetic approaches for cell labeling and manipulation. They mediate DNA strand exchange between two DNA molecules at dedicated recognition sites. Precise understanding of
Katherine Chuang +3 more
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Lineage specific recombination rates and microevolution in Listeria monocytogenes [PDF]
, 2008 Background: The bacterium Listeria monocytogenes is a saprotroph as well as an opportunistic human foodborne pathogen, which has previously been shown to consist of at least two widespread lineages (termed lineages I and II) and an uncommon lineage ...
Fortes, ED +9 more
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A method for producing transgenic cells using a multi-integrase system on a human artificial chromosome vector. [PDF]
PLoS ONE, 2011 The production of cells capable of expressing gene(s) of interest is important for a variety of applications in biomedicine and biotechnology, including gene therapy and animal transgenesis.
Shigeyuki Yamaguchi +5 more
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Non-Contiguous Protein Recombination [PDF]
, 2013 Swapping sequence elements among related proteins can produce chimeric proteins with novel behaviors and improved properties such as enhanced stability. Although homologous mutations are much more conservative than random mutations, chimeras of distantly-
Smith, Matthew Alexander
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An att site-based recombination reporter system for genome engineering and synthetic DNA assembly
BMC Biotechnology, 2017 Background Direct manipulation of the genome is a widespread technique for genetic studies and synthetic biology applications. The tyrosine and serine site-specific recombination systems of bacteriophages HK022 and ΦC31 are widely used for stable ...
Michael J. Bland +3 more
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Development of a novel in vitro assay for the evaluation of integron DNA integrase activity
Biotechnology & Biotechnological Equipment, 2016 Integrons play an important role in multidrug resistance. The integron platform codes for integrase (intI) that is required for gene cassette integration through site-specific recombination.
Fatemeh Tohidi +6 more
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Virulence, 2020 The proliferation of antibiotic resistance has its origins in horizontal gene transfer. The class 1 integrons mediate gene transfer by assimilating antibiotic-resistance genes through site-specific recombination.
Jun Zhang +8 more
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Recombination in enteroviruses is a biphasic replicative process involving the generation of greater-than genome length 'imprecise' intermediates [PDF]
, 2014 Recombination in enteroviruses provides an evolutionary mechanism for acquiring extensive regions of novel sequence, is suggested to have a role in genotype diversity and is known to have been key to the emergence of novel neuropathogenic variants of ...
David J Evans +13 more
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