Results 31 to 40 of about 446,158 (283)
A method for producing transgenic cells using a multi-integrase system on a human artificial chromosome vector. [PDF]
PLoS ONE, 2011 The production of cells capable of expressing gene(s) of interest is important for a variety of applications in biomedicine and biotechnology, including gene therapy and animal transgenesis.
Shigeyuki Yamaguchi +5 more
doaj +1 more source
A Topological Characterization Of Knots and Links Arising From Site-Specific Recombination [PDF]
, 1998 We develop a topological model of knots and links arising from a single (or multiple processive) round(s) of recombination starting with an unknot, unlink, or (2,m)-torus knot or link substrate.
Jones, JB +3 more
core +2 more sources
Recombination: Pieces of the site-specific recombination puzzle [PDF]
Current Biology, 1995 Understanding how nucleoprotein complexes interact with specific DNA sequences has come closer with structural and mechanistic studies of the interaction between the recombination enzyme resolvase and DNA.
Oram, M., Szczelkun, M.D., Halford, S.E.
openaire +2 more sources
An att site-based recombination reporter system for genome engineering and synthetic DNA assembly
BMC Biotechnology, 2017 Background Direct manipulation of the genome is a widespread technique for genetic studies and synthetic biology applications. The tyrosine and serine site-specific recombination systems of bacteriophages HK022 and ΦC31 are widely used for stable ...
Michael J. Bland +3 more
doaj +1 more source
Rapid metabolic pathway assembly and modification using serine integrase site-specific recombination [PDF]
, 2013 Synthetic biology requires effective methods to assemble DNA parts into devices and to modify these devices once made. Here we demonstrate a convenient rapid procedure for DNA fragment assembly using site-specific recombination by ϕC31 integrase.
Colloms, S.D. +7 more
core +4 more sources
Development of a novel in vitro assay for the evaluation of integron DNA integrase activity
Biotechnology & Biotechnological Equipment, 2016 Integrons play an important role in multidrug resistance. The integron platform codes for integrase (intI) that is required for gene cassette integration through site-specific recombination.
Fatemeh Tohidi +6 more
doaj +1 more source
Virulence, 2020 The proliferation of antibiotic resistance has its origins in horizontal gene transfer. The class 1 integrons mediate gene transfer by assimilating antibiotic-resistance genes through site-specific recombination.
Jun Zhang +8 more
doaj +1 more source
RAG-1 Mutations Associated with B-Cell-Negative SCID Dissociate the Nicking and Transesterification Steps of V(D)J Recombination [PDF]
, 2001 Some patients with B-cell-negative severe combined immune deficiency (SCID) carry mutations in RAG-1 or RAG-2 that impair V(D)J recombination. Two recessive RAG-1 mutations responsible for B-cell-negative SCID, R621H and E719K, impair V(D)J recombination
Chang, Fu-Chung +2 more
core +2 more sources
, 2007 Site-specific recombination is a reaction in which a pair of genetically defined sites undergoes reciprocal exchange ("crossing-over") via a recombinase-mediated DNA breakage and joining process. Such reactions have a wide range of biological outcomes, from integration and excision of virus genomes into and out of host chromosomes, to acquisition of ...
Grainge, I, Sherratt, D
openaire +2 more sources
Site-specific recombination in plane view [PDF]
Structure, 1997 Biochemists have worked long and hard on each reaction component and chemical step to reach the point of asking the question as to how protein and DNA molecules are arranged and rearranged in the process of site-specific recombination. The structures of several lambda integrase family members published recently have answered many of the questions about
Yang, Wei, Mizuuchi, Kiyoshi
openaire +2 more sources