Lineage specific recombination rates and microevolution in Listeria monocytogenes [PDF]
, 2008 Background: The bacterium Listeria monocytogenes is a saprotroph as well as an opportunistic human foodborne pathogen, which has previously been shown to consist of at least two widespread lineages (termed lineages I and II) and an uncommon lineage ...
Fortes, ED +9 more
core +1 more source
Non-Contiguous Protein Recombination [PDF]
, 2013 Swapping sequence elements among related proteins can produce chimeric proteins with novel behaviors and improved properties such as enhanced stability. Although homologous mutations are much more conservative than random mutations, chimeras of distantly-
Smith, Matthew Alexander
core +1 more source
Characterization of the integration and modular excision of the integrative conjugative element PAISt in Streptomyces turgidiscabies Car8. [PDF]
PLoS ONE, 2014 PAISt is a large genomic island located in the chromosome of the plant pathogen Streptomyces turgidiscabies Car8. The island carries clustered virulence genes, transfers to other Streptomyces species, and integrates by site-specific recombination at the ...
Jose C Huguet-Tapia +2 more
doaj +1 more source
Control of phage Bxb1 excision by a novel recombination directionality factor. [PDF]
PLoS Biology, 2006 Mycobacteriophage Bxb1 integrates its DNA at the attB site of the Mycobacterium smegmatis genome using the viral attP site and a phage-encoded integrase generating the recombinant junctions attL and attR.
Pallavi Ghosh +2 more
doaj +1 more source
Recombination in enteroviruses is a biphasic replicative process involving the generation of greater-than genome length 'imprecise' intermediates [PDF]
, 2014 Recombination in enteroviruses provides an evolutionary mechanism for acquiring extensive regions of novel sequence, is suggested to have a role in genotype diversity and is known to have been key to the emergence of novel neuropathogenic variants of ...
David J Evans +13 more
core +1 more source
In vivo site-specific recombination using the β-rec/six system
BioTechniques, 2008 The prokaryotic β serine recombinase (β-rec) catalyzes site-specific recombination between two directly oriented six sites (93 bp) in mammalian cells, both in episomal and in chromosomally integrated substrates.
Pilar Servert +9 more
doaj +1 more source
Positive-negative-selection-mediated gene targeting in rice
Frontiers in Plant Science, 2015 Gene targeting (GT) refers to the designed modification of genomic sequence(s) through homologous recombination (HR). GT is a powerful tool both for the study of gene function and for molecular breeding.
Zenpei eShimatani +4 more
doaj +1 more source
Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
Cis- and trans-acting elements regulate the mouse Psmb9 meiotic recombination hotspot. [PDF]
PLoS Genetics, 2007 In most eukaryotes, the prophase of the first meiotic division is characterized by a high level of homologous recombination between homologous chromosomes.
Frédéric Baudat, Bernard de Massy
doaj +1 more source
Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics
FEBS Letters, EarlyView.Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt +8 more
wiley +1 more source