Characterization of the integration and modular excision of the integrative conjugative element PAISt in Streptomyces turgidiscabies Car8. [PDF]
PLoS ONE, 2014 PAISt is a large genomic island located in the chromosome of the plant pathogen Streptomyces turgidiscabies Car8. The island carries clustered virulence genes, transfers to other Streptomyces species, and integrates by site-specific recombination at the ...
Jose C Huguet-Tapia +2 more
doaj +1 more source
New methods for tightly regulated gene expression and highly efficient chromosomal integration of cloned genes for Methanosarcina species [PDF]
, 2008 A highly efficient method for chromosomal integration of cloned DNA into Methanosarcina spp. was developed utilizing the site-specific recombination system from the Streptomyces phage PhiC31. Host strains expressing the PhiC31 integrase gene and carrying
Guss, Adam M. +4 more
core +1 more source
Control of phage Bxb1 excision by a novel recombination directionality factor. [PDF]
PLoS Biology, 2006 Mycobacteriophage Bxb1 integrates its DNA at the attB site of the Mycobacterium smegmatis genome using the viral attP site and a phage-encoded integrase generating the recombinant junctions attL and attR.
Pallavi Ghosh +2 more
doaj +1 more source
Efficient CRISPR-rAAV engineering of endogenous genes to study protein function by allele-specific RNAi. [PDF]
, 2015 Gene knockout strategies, RNAi and rescue experiments are all employed to study mammalian gene function. However, the disadvantages of these approaches include: loss of function adaptation, reduced viability and gene overexpression that rarely matches ...
Dowdy, Steven F +5 more
core +1 more source
Specificity in V(D)J recombination: new lessons from biochemistry and genetics [PDF]
, 1997 Recent in vitro work on V(D)J recombination has helped to clarify its mechanism. The first stage of the reaction, which can be reproduced with the purified RAG1 and RAG2 proteins, is a site-specific cleavage that generates the same ...
Gellert, M. +2 more
core +3 more sources
In vivo site-specific recombination using the β-rec/six system
BioTechniques, 2008 The prokaryotic β serine recombinase (β-rec) catalyzes site-specific recombination between two directly oriented six sites (93 bp) in mammalian cells, both in episomal and in chromosomally integrated substrates.
Pilar Servert +9 more
doaj +1 more source
Two different evolutionary lines of filamentous phages in Ralstonia solanacearum: their effects on bacterial virulence [PDF]
, 2015 The integration and excision of various filamentous phage genomes into and out of their host chromosomes occurs by site-specific recombination. The mechanisms proposed for these events include reactions mediated by phage-encoded recombinases and host ...
Ahmed Askora, Takashi Yamada
core +2 more sources
Ligand-regulated site-specific recombination. [PDF]
Proceedings of the National Academy of Sciences, 1995 Site-specific recombination offers a potential way to alter a living genome by design in a precise and stable manner. This potential requires strategies which can be used to regulate the recombination event. We describe a strategy to regulate FLP recombinase activity which relies on expressing FLP as a fusion protein with steroid hormone receptor ...
C, Logie, A F, Stewart
openaire +2 more sources
Positive-negative-selection-mediated gene targeting in rice
Frontiers in Plant Science, 2015 Gene targeting (GT) refers to the designed modification of genomic sequence(s) through homologous recombination (HR). GT is a powerful tool both for the study of gene function and for molecular breeding.
Zenpei eShimatani +4 more
doaj +1 more source
High-resolution mapping of heteroduplex DNA formed during UV-induced and spontaneous mitotic recombination events in yeast. [PDF]
, 2017 In yeast, DNA breaks are usually repaired by homologous recombination (HR). An early step for HR pathways is formation of a heteroduplex, in which a single-strand from the broken DNA molecule pairs with a strand derived from an intact DNA molecule.
Dominska, Margaret +3 more
core +3 more sources