Results 41 to 50 of about 758 (215)

Efficient site-specific recombination by self-activating split-Dre recombinase in mammalian cells and E. coli

Journal of Biological Engineering
Background Site-specific recombination (SSR) systems are essential tools for conditional genetic manipulation and are valued for their efficacy and user friendliness. However, the development of novel SSR strategies is urgently needed.
Chichu Xie, Jinfeng Gan, Yuanhao Zhang, Wentao Yu, Ruirui Li, Dan Yu, Yu Liu, Song Guo Zheng, Guangying Qi   +8 more
doaj   +1 more source

Single-Molecule Tethered Particle Motion: Stepwise Analyses of Site-Specific DNA Recombination

Micromachines, 2018
Tethered particle motion/microscopy (TPM) is a biophysical tool used to analyze changes in the effective length of a polymer, tethered at one end, under changing conditions.
Hsiu-Fang Fan, Chien-Hui Ma, Makkuni Jayaram   +2 more
doaj   +1 more source

The integrase of genomic island GIsul2 mediates the mobilization of GIsul2 and ISCR-related element CR2-sul2 unit through site-specific recombination

Frontiers in Microbiology, 2022
In the worldwide health threat posed by antibiotic-resistant bacterial pathogens, mobile genetic elements (MGEs) play a critical role in favoring the dissemination of resistance genes.
Gang Zhang, Qinna Cui, Qinna Cui, Jianjuan Li, Jianjuan Li, Ruiliang Guo, Ruiliang Guo, Sébastien Olivier Leclercq, Lifeng Du, Na Tang, Na Tang, Yuqin Song, Chao Wang, Fangqing Zhao, Jie Feng   +14 more
doaj   +1 more source

Spatial biology in cancer epigenetics

Molecular Oncology, EarlyView.
Spatial epigenomics combines molecular profiling with tissue architecture to reveal how gene regulation is organized within intact tissues. In cancer, these technologies uncover the mechanisms driving tumor heterogeneity and microenvironmental interactions, opening new opportunities for biomarker discovery and precision medicine.
Eva Crespo‐García, Manel Esteller
wiley   +1 more source

Efficient Cre/loxP Site-Specific Recombination in a HepG2 Human Liver Cell Line

Cell Transplantation, 2000
A worldwide shortage of donor livers is a limiting factor of the clinical application of hepatocyte transplantation (HTX). To resolve this issue, we focused on a reversible immortalization system that allows temporary expansion of primary hepatocyte ...
Naoya Kobayashi M.D., Ph.D., Hirofumi Noguchi, Karen A. Westerman, Toshihisa Matsumura, Takamasa Watanabe, Toshinori Totsugawa, Toshiyoshi Fujiwara, Philippe Leboulch, Noriaki Tanaka   +8 more
doaj   +1 more source

The site-specific recombination system of actinophage TG1 [PDF]

FEMS Microbiology Letters, 2009
Actinophage TG1 forms stable lysogens by integrating at a unique site on chromosomes of Streptomyces strains. The phage (attP(TG1)) and bacterial (attB(TG1)) attachment sites for TG1 were deduced from comparative genomic studies on the TG1-lysogen and nonlysogen of Streptomyces avermitilis.
Kentaro, Morita, Tomoyuki, Yamamoto, Naoki, Fusada, Mamoru, Komatsu, Haruo, Ikeda, Nobutaka, Hirano, Hideo, Takahashi   +6 more
openaire   +2 more sources

Cancer cell‐intrinsic type 1 interferon: production, signaling, and outcomes within sex‐biased, female malignancies

Molecular Oncology, EarlyView.
The cell‐autonomous roles of interferon type 1 vary based on duration and intensity. While acute, robust signaling results in cancer cell cytotoxic effects, sustained, low‐level signaling is associated with tumor‐promoting effects. This review summarizes current research of IFN‐1 in cancer and within the clinical setting, with an emphasis on female ...
Ashlyn Conant, Nora Badiner, Sharon Asariah, Kiera McGivney, Vishwa Shah, Tise Suzuki, Yevgeniya J. Ioffe, Juli J. Unternaehrer   +7 more
wiley   +1 more source

Enhanced cell-permeant Cre protein for site-specific recombination in cultured cells

BMC Biotechnology, 2004
Background Cell-permeant Cre DNA site-specific recombinases provide an easily controlled means to regulate gene structure and function in living cells. Since recombination provides a stable and unambiguous record of protein uptake, the enzyme may also be
Ruley H Earl, Gebre-Amlak Kassatihun D, Jo Daewoong, Lin Qing   +3 more
doaj   +1 more source

Integrase-mediated recombination of the veb1 gene cassette encoding an extended-spectrum β-lactamase. [PDF]

PLoS ONE, 2012
The veb1 gene cassette encodes the extended spectrum β-lactamase, VEB-1 that is increasingly isolated from worldwide Gram-negative rods. Veb1 is commonly inserted into the variable region of different class 1 integrons in which it is always associated ...
Daniel Aubert, Thierry Naas, Patrice Nordmann   +2 more
doaj   +1 more source

ADP‐ribosylation: An emerging regulator of the epigenome

Molecular Oncology, EarlyView.
ADP‐ribosylation has emerged as a dynamic epigenetic signaling mechanism that modifies histones and chromatin‐associated proteins. Through coordinated PARylation and MARylation, it integrates with other histone modifications to regulate chromatin structure, transcription factor activity, and gene expression, influencing genome function and disease ...
Cristel V. Camacho, Chenqian Liu, W. Lee Kraus   +2 more
wiley   +1 more source