Results 21 to 30 of about 446,158 (283)
The λ Integrase Site-specific Recombination Pathway. [PDF]
Microbiol Spectr, 2015 ABSTRACTThe site-specific recombinase encoded by bacteriophage λ (Int) is responsible for integrating and excising the viral chromosome into and out of the chromosome of itsEscherichia colihost. Int carries out a reaction that is highly directional, tightly regulated, and depends upon an ensemble of accessory DNA bending proteins acting on 240 bp of ...
Landy A.
europepmc +4 more sources
Application of the Cre/loxP Site-Specific Recombination System for Gene Transformation in Aurantiochytrium limacinum [PDF]
Molecules, 2015 The Cre/loxP site-specific recombination system was applied to Aurantiochytrium limacinum to obtain a transformant without the antibiotic resistance marker gene.
Hengyi Sun +8 more
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Trade-off between canonical and unusual recombination sites promotes diversity and stability of gene cassette arrays of mobile integrons [PDF]
Scientific ReportsMobile integrons are the most efficient mechanism of Gram-negative bacteria to resist antimicrobial changing pressures in the nosocomial niche. Integron’s integrases mediate site-specific recombination of distinct DNA structures such as attI, attC and ...
Adrián Gonzales Machuca +5 more
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Temporally-controlled site-specific recombination in zebrafish. [PDF]
PLoS ONE, 2009 Conventional use of the site-specific recombinase Cre is a powerful technology in mouse, but almost absent in other vertebrate model organisms. In zebrafish, Cre-mediated recombination efficiency was previously very low.
Stefan Hans +4 more
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Predicting algorithm of attC site based on combination optimization strategy
Connection Science, 2022 Site-specific recombination systems are widely used as bioengineering tools. However, the traditional site-specific recombination system requires a consensus sequence for the specific site.
Zhendong Liu +9 more
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Nature, 1981 Site-specific recombination is a reaction in which a pair of genetically defined sites undergoes reciprocal exchange (“crossing-over”) via a recombinase-mediated DNA breakage and joining process. Such reactions have a wide range of biological outcomes, from integration and excision of virus genomes into and out of host chromosomes, to acquisition of ...
David Sherratt, Avril Arthur, Paul Dyson
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High-efficiency FLP and PhiC31 site-specific recombination in mammalian cells. [PDF]
PLoS ONE, 2007 DNA site-specific recombinases (SSRs) such as Cre, FLPe, and phiC31, are powerful tools for analyzing gene function in vertebrates. While the availability of multiple high-efficiency SSRs would facilitate a wide array of genomic engineering possibilities,
Christopher S Raymond, Philippe Soriano
doaj +1 more source
BioTechniques, 2011 Site-specific recombination is a powerful biotechnological tool for genome engineering. We previously reported two novel site-specific recombination systems, VCre/VloxP and SCre/SloxP, that do not cross-react with Cre/loxP and Flp/FRT in culture cells ...
Sachiko Minorikawa, Manabu Nakayama
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Making serine integrases work for us [PDF]
, 2017 DNA site-specific recombinases are enzymes (often associated with mobile DNA elements) that catalyse breaking and rejoining of DNA strands at specific points, thereby bringing about precise genetic rearrangements.
Stark, W. Marshall
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Novel Heterotypic Rox Sites for Combinatorial Dre Recombination Strategies
G3: Genes, Genomes, Genetics, 2016 Site-specific recombinases (SSRs) such as Cre are widely used in gene targeting and genetic approaches for cell labeling and manipulation. They mediate DNA strand exchange between two DNA molecules at dedicated recognition sites. Precise understanding of
Katherine Chuang +3 more
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