Artificial nondirectional site-specific recombination systems [PDF]
iScience, 2022 Summary: Site-specific recombination systems (SRSs) are widely used in studies on synthetic biology and related disciplines. Nondirectional SRSs can randomly trigger excision, integration, reversal, and translocation, which are effective tools to achieve
Jun-Yi Wang +9 more
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Model-guided engineering of DNA sequences with predictable site-specific recombination rates [PDF]
Nature Communications, 2022 Site-specific recombination (SSR) is an important tool in synthetic biology, but its applications are limited by the inability to predictably tune SSR reaction rates.
Qiuge Zhang +2 more
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The Site-Specific Recombination System of the Escherichia coli Bacteriophage Φ24B [PDF]
Frontiers in Microbiology, 2020 Stx bacteriophages are members of the lambdoid group of phages and are responsible for Shiga toxin (Stx) production and the dissemination of Shiga toxin genes (stx) across shigatoxigenic Escherichia coli (STEC).
Mohammed Radhi Mohaisen +4 more
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Compatibility of Site-Specific Recombination Units between Mobile Genetic Elements [PDF]
iScience, 2020 Summary: Site-specific recombination (SSR) systems are employed for transfer of mobile genetic elements (MGEs), such as lysogenic phages and integrative conjugative elements (ICEs).
Shota Suzuki +5 more
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Efficient site-specific recombination by self-activating split-Dre recombinase in mammalian cells and E. coli [PDF]
Journal of Biological EngineeringBackground Site-specific recombination (SSR) systems are essential tools for conditional genetic manipulation and are valued for their efficacy and user friendliness. However, the development of novel SSR strategies is urgently needed.
Chichu Xie +8 more
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Bacteriophage lambda site-specific recombination. [PDF]
Mol MicrobiolAbstractThe site‐specific recombination pathway of bacteriophage λ encompasses isoenergetic but highly directional and tightly regulated integrative and excisive reactions that integrate and excise the vial chromosome into and out of the bacterial chromosome.
Van Duyne GD, Landy A.
europepmc +3 more sources
phiC31 integrase-mediated site-specific recombination in barley. [PDF]
PLoS ONE, 2012 The Streptomyces phage phiC31 integrase was tested for its feasibility in excising transgenes from the barley genome through site-specific recombination.
Eszter Kapusi +4 more
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Genome-wide detection of conservative site-specific recombination in bacteria. [PDF]
PLoS Genetics, 2018 The ability of clonal bacterial populations to generate genomic and phenotypic heterogeneity is thought to be of great importance for many commensal and pathogenic bacteria. One common mechanism contributing to diversity formation relies on the inversion
Ognjen Sekulovic +5 more
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The integrase of genomic island GIsul2 mediates the mobilization of GIsul2 and ISCR-related element CR2-sul2 unit through site-specific recombination [PDF]
Frontiers in Microbiology, 2022 In the worldwide health threat posed by antibiotic-resistant bacterial pathogens, mobile genetic elements (MGEs) play a critical role in favoring the dissemination of resistance genes.
Gang Zhang +14 more
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Large T antigen mediated target gene replication improves site-specific recombination efficiency [PDF]
Frontiers in Bioengineering and BiotechnologyWith advantages of high-fidelity, monoclonality and large cargo capacity, site-specific recombination (SSR) holds great promises for precise genomic modifications.
Zening Wang +4 more
doaj +2 more sources