Results 1 to 10 of about 758 (215)

Attachment Site Selection and Identity in Bxb1 Serine Integrase-Mediated Site-Specific Recombination [PDF]

PLoS Genetics, 2013
Phage-encoded serine integrases mediate directionally regulated site-specific recombination between short attP and attB DNA sites without host factor requirements.
Graham Hatfull, Pallavi Ghosh
exaly   +2 more sources

Application of the Cre/loxP Site-Specific Recombination System for Gene Transformation in Aurantiochytrium limacinum

Molecules, 2015
The Cre/loxP site-specific recombination system was applied to Aurantiochytrium limacinum to obtain a transformant without the antibiotic resistance marker gene.
Fei Wu, Bing-Bing Zhou, Xiaonan Zang
exaly   +3 more sources

Artificial nondirectional site-specific recombination systems

iScience, 2022
Summary: Site-specific recombination systems (SRSs) are widely used in studies on synthetic biology and related disciplines. Nondirectional SRSs can randomly trigger excision, integration, reversal, and translocation, which are effective tools to achieve
Jun-Yi Wang, Yue-Yang Cao, Ya-Nan Chen, Xiao-Le Wu, Bo-Tao He, Si-Yu Zhu, Xiao Zhou, Yi Wu, Bing-Zhi Li, Ying-Jin Yuan   +9 more
doaj   +1 more source

Temporally-controlled site-specific recombination in zebrafish. [PDF]

PLoS ONE, 2009
Conventional use of the site-specific recombinase Cre is a powerful technology in mouse, but almost absent in other vertebrate model organisms. In zebrafish, Cre-mediated recombination efficiency was previously very low.
Stefan Hans, Jan Kaslin, Dorian Freudenreich, Michael Brand, Michael Brand   +4 more
doaj   +1 more source

Recombination: Pieces of the site-specific recombination puzzle [PDF]

Current Biology, 1995
Understanding how nucleoprotein complexes interact with specific DNA sequences has come closer with structural and mechanistic studies of the interaction between the recombination enzyme resolvase and DNA.
Oram, M., Szczelkun, M.D., Halford, S.E.
openaire   +2 more sources

phiC31 integrase-mediated site-specific recombination in barley. [PDF]

PLoS ONE, 2012
The Streptomyces phage phiC31 integrase was tested for its feasibility in excising transgenes from the barley genome through site-specific recombination.
Eszter Kapusi, Katja Kempe, Myroslava Rubtsova, Jochen Kumlehn, Mario Gils   +4 more
doaj   +1 more source

Predicting algorithm of attC site based on combination optimization strategy

Connection Science, 2022
Site-specific recombination systems are widely used as bioengineering tools. However, the traditional site-specific recombination system requires a consensus sequence for the specific site.
Zhendong Liu, Xi Chen, Dongyan Li, Xinrong Lv, Mengying Qin, Ke Bai, Zhiqiang He, Yurong Yang, Xiaofeng Li, Qionghai Dai   +9 more
doaj   +1 more source

FLP recombinase-mediated site-specific recombination in silkworm, Bombyx mori. [PDF]

PLoS ONE, 2012
A comprehensive understanding of gene function and the production of site-specific genetically modified mutants are two major goals of genetic engineering in the post-genomic era.
Ding-Pei Long, Ai-Chun Zhao, Xue-Jiao Chen, Yang Zhang, Wei-Jian Lu, Qing Guo, Alfred M Handler, Zhong-Huai Xiang   +7 more
doaj   +1 more source

Model-guided engineering of DNA sequences with predictable site-specific recombination rates

Nature Communications, 2022
Site-specific recombination (SSR) is an important tool in synthetic biology, but its applications are limited by the inability to predictably tune SSR reaction rates.
Qiuge Zhang, Samira M. Azarin, Casim A. Sarkar   +2 more
doaj   +1 more source

High-efficiency FLP and PhiC31 site-specific recombination in mammalian cells. [PDF]

PLoS ONE, 2007
DNA site-specific recombinases (SSRs) such as Cre, FLPe, and phiC31, are powerful tools for analyzing gene function in vertebrates. While the availability of multiple high-efficiency SSRs would facilitate a wide array of genomic engineering possibilities,
Christopher S Raymond, Philippe Soriano
doaj   +1 more source