FLP recombinase-mediated site-specific recombination in silkworm, Bombyx mori. [PDF]
PLoS One, 2012 A comprehensive understanding of gene function and the production of site-specific genetically modified mutants are two major goals of genetic engineering in the post-genomic era.
Long DP +7 more
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Genome-wide detection of conservative site-specific recombination in bacteria. [PDF]
PLoS Genet, 2018 The ability of clonal bacterial populations to generate genomic and phenotypic heterogeneity is thought to be of great importance for many commensal and pathogenic bacteria. One common mechanism contributing to diversity formation relies on the inversion
Sekulovic O +5 more
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Artificial nondirectional site-specific recombination systems. [PDF]
iScience, 2022 Summary: Site-specific recombination systems (SRSs) are widely used in studies on synthetic biology and related disciplines. Nondirectional SRSs can randomly trigger excision, integration, reversal, and translocation, which are effective tools to achieve
Wang JY +9 more
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Model-guided engineering of DNA sequences with predictable site-specific recombination rates. [PDF]
Nat Commun, 2022 Site-specific recombination (SSR) is an important tool in synthetic biology, but its applications are limited by the inability to predictably tune SSR reaction rates.
Zhang Q, Azarin SM, Sarkar CA.
europepmc +2 more sources
The Site-Specific Recombination System of the Escherichia coli Bacteriophage Φ24B. [PDF]
Front Microbiol, 2020 Stx bacteriophages are members of the lambdoid group of phages and are responsible for Shiga toxin (Stx) production and the dissemination of Shiga toxin genes (stx) across shigatoxigenic Escherichia coli (STEC).
Mohaisen MR +3 more
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Compatibility of Site-Specific Recombination Units between Mobile Genetic Elements. [PDF]
iScience, 2020 Summary: Site-specific recombination (SSR) systems are employed for transfer of mobile genetic elements (MGEs), such as lysogenic phages and integrative conjugative elements (ICEs).
Suzuki S +5 more
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Efficient site-specific recombination by self-activating split-Dre recombinase in mammalian cells and E. coli. [PDF]
J Biol EngBackground Site-specific recombination (SSR) systems are essential tools for conditional genetic manipulation and are valued for their efficacy and user friendliness. However, the development of novel SSR strategies is urgently needed.
Xie C +8 more
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phiC31 integrase-mediated site-specific recombination in barley. [PDF]
PLoS One, 2012 The Streptomyces phage phiC31 integrase was tested for its feasibility in excising transgenes from the barley genome through site-specific recombination.
Kapusi E +4 more
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, 2007 Site-specific recombination is a reaction in which a pair of genetically defined sites undergoes reciprocal exchange ("crossing-over") via a recombinase-mediated DNA breakage and joining process. Such reactions have a wide range of biological outcomes, from integration and excision of virus genomes into and out of host chromosomes, to acquisition of ...
Grainge, I, Sherratt, D
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Determinants of selectivity in Xer site-specific recombination. [PDF]
Genes & Development, 1996 A remarkable property of some DNA-binding proteins that can interact with and pair distant DNA segments is that they mediate their biological function only when their binding sites are arranged in a specific configuration. Xer site-specific recombination at natural plasmid recombination sites (e.g., cer in ColE1) is preferentially intramolecular ...
Blakely, G, Sherratt, D
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