Artificial nondirectional site-specific recombination systems [PDF]
iScience, 2022 Summary: Site-specific recombination systems (SRSs) are widely used in studies on synthetic biology and related disciplines. Nondirectional SRSs can randomly trigger excision, integration, reversal, and translocation, which are effective tools to achieve
Jun-Yi Wang +9 more
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Attachment site selection and identity in Bxb1 serine integrase-mediated site-specific recombination. [PDF]
PLoS Genetics, 2013 Phage-encoded serine integrases mediate directionally regulated site-specific recombination between short attP and attB DNA sites without host factor requirements.
Shweta Singh +2 more
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Model-guided engineering of DNA sequences with predictable site-specific recombination rates [PDF]
Nature Communications, 2022 Site-specific recombination (SSR) is an important tool in synthetic biology, but its applications are limited by the inability to predictably tune SSR reaction rates.
Qiuge Zhang +2 more
doaj +2 more sources
The Site-Specific Recombination System of the Escherichia coli Bacteriophage Φ24B [PDF]
Frontiers in Microbiology, 2020 Stx bacteriophages are members of the lambdoid group of phages and are responsible for Shiga toxin (Stx) production and the dissemination of Shiga toxin genes (stx) across shigatoxigenic Escherichia coli (STEC).
Mohammed Radhi Mohaisen +4 more
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Compatibility of Site-Specific Recombination Units between Mobile Genetic Elements [PDF]
iScience, 2020 Summary: Site-specific recombination (SSR) systems are employed for transfer of mobile genetic elements (MGEs), such as lysogenic phages and integrative conjugative elements (ICEs).
Shota Suzuki +5 more
doaj +2 more sources
Efficient site-specific recombination by self-activating split-Dre recombinase in mammalian cells and E. coli [PDF]
Journal of Biological EngineeringBackground Site-specific recombination (SSR) systems are essential tools for conditional genetic manipulation and are valued for their efficacy and user friendliness. However, the development of novel SSR strategies is urgently needed.
Chichu Xie +8 more
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Mapping replication dynamics in Trypanosoma brucei reveals a link with telomere transcription and antigenic variation [PDF]
eLife, 2016 Survival of Trypanosoma brucei depends upon switches in its protective Variant Surface Glycoprotein (VSG) coat by antigenic variation. VSG switching occurs by frequent homologous recombination, which is thought to require locus-specific initiation. Here,
Rebecca Devlin +8 more
doaj +4 more sources
Bacteriophage lambda site-specific recombination. [PDF]
Mol MicrobiolAbstractThe site‐specific recombination pathway of bacteriophage λ encompasses isoenergetic but highly directional and tightly regulated integrative and excisive reactions that integrate and excise the vial chromosome into and out of the bacterial chromosome.
Van Duyne GD, Landy A.
europepmc +3 more sources
phiC31 integrase-mediated site-specific recombination in barley. [PDF]
PLoS ONE, 2012 The Streptomyces phage phiC31 integrase was tested for its feasibility in excising transgenes from the barley genome through site-specific recombination.
Eszter Kapusi +4 more
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Structural snapshots of Xer recombination reveal activation by synaptic complex remodeling and DNA bending [PDF]
eLife, 2016 Bacterial Xer site-specific recombinases play an essential genome maintenance role by unlinking chromosome multimers, but their mechanism of action has remained structurally uncharacterized. Here, we present two high-resolution structures of Helicobacter
Aleksandra Bebel +4 more
doaj +6 more sources