, 2007 Site-specific recombination is a reaction in which a pair of genetically defined sites undergoes reciprocal exchange ("crossing-over") via a recombinase-mediated DNA breakage and joining process. Such reactions have a wide range of biological outcomes, from integration and excision of virus genomes into and out of host chromosomes, to acquisition of ...
Grainge, I, Sherratt, D
openaire +3 more sources
Attachment site selection and identity in Bxb1 serine integrase-mediated site-specific recombination. [PDF]
PLoS Genetics, 2013 Phage-encoded serine integrases mediate directionally regulated site-specific recombination between short attP and attB DNA sites without host factor requirements.
Shweta Singh +2 more
doaj +3 more sources
Determinants of selectivity in Xer site-specific recombination. [PDF]
Genes & Development, 1996 A remarkable property of some DNA-binding proteins that can interact with and pair distant DNA segments is that they mediate their biological function only when their binding sites are arranged in a specific configuration. Xer site-specific recombination at natural plasmid recombination sites (e.g., cer in ColE1) is preferentially intramolecular ...
Blakely, G, Sherratt, D
openaire +5 more sources
Engineering integrative vectors based on phage site-specific recombination mechanism for Lactococcus lactis [PDF]
BMC Biotechnology, 2019 Background Site-specific integration system allows foreign DNA to be integrated into the specific site of the host genome, enabling stable expression of heterologous protein.
Innanurdiani Koko +3 more
doaj +2 more sources
Orthogonal LoxPsym sites allow multiplexed site-specific recombination in prokaryotic and eukaryotic hosts [PDF]
Nature CommunicationsSite-specific recombinases such as the Cre-LoxP system are routinely used for genome engineering in both prokaryotes and eukaryotes. Importantly, recombinases complement the CRISPR-Cas toolbox and provide the additional benefit of high-efficiency DNA ...
Charlotte Cautereels +9 more
doaj +2 more sources
The λ Integrase Site-specific Recombination Pathway. [PDF]
Microbiol Spectr, 2015 ABSTRACTThe site-specific recombinase encoded by bacteriophage λ (Int) is responsible for integrating and excising the viral chromosome into and out of the chromosome of itsEscherichia colihost. Int carries out a reaction that is highly directional, tightly regulated, and depends upon an ensemble of accessory DNA bending proteins acting on 240 bp of ...
Landy A.
europepmc +4 more sources
Control of directionality in Streptomyces phage φBT1 integrase-mediated site-specific recombination. [PDF]
PLoS ONE, 2013 Streptomyces phage φBT1 integrates its genome into the attB site of the host chromosome with the attP site to generate attL and attR. The φBT1 integrase belongs to the large serine recombinase subfamily which directly binds to target sites to initiate ...
Lin Zhang +4 more
doaj +2 more sources
Utilization of Site-Specific Recombination in Biopharmaceutical Production. [PDF]
Iran Biomed J, 2016 Mammalian expression systems, due to their capacity in post-translational modification, are preferred systems for biopharmaceutical protein production. Several recombinant protein systems have been introduced to the market, most of which are under clinical development.
Ahmadi M +3 more
europepmc +3 more sources
Application of the Cre/loxP Site-Specific Recombination System for Gene Transformation in Aurantiochytrium limacinum [PDF]
Molecules, 2015 The Cre/loxP site-specific recombination system was applied to Aurantiochytrium limacinum to obtain a transformant without the antibiotic resistance marker gene.
Hengyi Sun +8 more
doaj +2 more sources
Use of a unique reporter cassette to examine 5´ to 3´ resection at a site specific DNA double-strand break during meiosis [PDF]
, 2006 Meiotic recombination in Saccharomyces cerevisiae is initiated by the formation of DNA double strand breaks (DSBs), which are created by Spoil protein.
Bishop-Bailey, Anna
core +6 more sources