The integrase of genomic island GIsul2 mediates the mobilization of GIsul2 and ISCR-related element CR2-sul2 unit through site-specific recombination [PDF]
Frontiers in Microbiology, 2022 In the worldwide health threat posed by antibiotic-resistant bacterial pathogens, mobile genetic elements (MGEs) play a critical role in favoring the dissemination of resistance genes.
Gang Zhang +14 more
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Genome-wide detection of conservative site-specific recombination in bacteria. [PDF]
PLoS Genetics, 2018 The ability of clonal bacterial populations to generate genomic and phenotypic heterogeneity is thought to be of great importance for many commensal and pathogenic bacteria. One common mechanism contributing to diversity formation relies on the inversion
Ognjen Sekulovic +5 more
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Large T antigen mediated target gene replication improves site-specific recombination efficiency [PDF]
Frontiers in Bioengineering and BiotechnologyWith advantages of high-fidelity, monoclonality and large cargo capacity, site-specific recombination (SSR) holds great promises for precise genomic modifications.
Zening Wang +4 more
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Gated rotation mechanism of site-specific recombination by ϕC31 integrase. [PDF]
Proc Natl Acad Sci U S A, 2012 Integrases, such as that of the Streptomyces temperate bacteriophage ϕC31, promote site-specific recombination between DNA sequences in the bacteriophage and bacterial genomes to integrate or excise the phage DNA.
Olorunniji FJ +6 more
europepmc +3 more sources
FLP recombinase-mediated site-specific recombination in silkworm, Bombyx mori. [PDF]
PLoS ONE, 2012 A comprehensive understanding of gene function and the production of site-specific genetically modified mutants are two major goals of genetic engineering in the post-genomic era.
Ding-Pei Long +7 more
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Engineering integrative vectors based on phage site-specific recombination mechanism for Lactococcus lactis [PDF]
BMC Biotechnology, 2019 Background Site-specific integration system allows foreign DNA to be integrated into the specific site of the host genome, enabling stable expression of heterologous protein.
Innanurdiani Koko +3 more
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Control of directionality in Streptomyces phage φBT1 integrase-mediated site-specific recombination. [PDF]
PLoS ONE, 2013 Streptomyces phage φBT1 integrates its genome into the attB site of the host chromosome with the attP site to generate attL and attR. The φBT1 integrase belongs to the large serine recombinase subfamily which directly binds to target sites to initiate ...
Lin Zhang +4 more
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Pandemic Vibrio cholerae shuts down site-specific recombination to retain an interbacterial defence mechanism [PDF]
Nature Communications, 2020 Vibrio cholerae uses a type VI secretion system (T6SS) to kill neighbouring competitors. Here, Santoriello et al. show that a T6SS gene cluster (Aux3) exists as a mobile, prophage-like element in some environmental strains, and as a stable truncated form
Francis J. Santoriello +3 more
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Orthogonal LoxPsym sites allow multiplexed site-specific recombination in prokaryotic and eukaryotic hosts [PDF]
Nature CommunicationsSite-specific recombinases such as the Cre-LoxP system are routinely used for genome engineering in both prokaryotes and eukaryotes. Importantly, recombinases complement the CRISPR-Cas toolbox and provide the additional benefit of high-efficiency DNA ...
Charlotte Cautereels +9 more
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Dynamics in Cre-loxP site-specific recombination. [PDF]
Curr Opin Struct BiolCre recombinase is a phage-derived enzyme that has found utility for precise manipulation of DNA sequences. Cre recognizes and recombines pairs of loxP sequences characterized by an inverted repeat and asymmetric spacer. Cre cleaves and religates its DNA targets such that error-prone repair pathways are not required to generate intact DNA products ...
Foster MP +3 more
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