Results 11 to 20 of about 758 (215)
BioTechniques, 2011 Site-specific recombination is a powerful biotechnological tool for genome engineering. We previously reported two novel site-specific recombination systems, VCre/VloxP and SCre/SloxP, that do not cross-react with Cre/loxP and Flp/FRT in culture cells ...
Sachiko Minorikawa, Manabu Nakayama
doaj +1 more source
Compatibility of Site-Specific Recombination Units between Mobile Genetic Elements
iScience, 2020 Summary: Site-specific recombination (SSR) systems are employed for transfer of mobile genetic elements (MGEs), such as lysogenic phages and integrative conjugative elements (ICEs).
Shota Suzuki +5 more
doaj +1 more source
Novel Heterotypic Rox Sites for Combinatorial Dre Recombination Strategies
G3: Genes, Genomes, Genetics, 2016 Site-specific recombinases (SSRs) such as Cre are widely used in gene targeting and genetic approaches for cell labeling and manipulation. They mediate DNA strand exchange between two DNA molecules at dedicated recognition sites. Precise understanding of
Katherine Chuang +3 more
doaj +1 more source
Xer site‐specific recombination in vitro. [PDF]
The EMBO Journal, 1995 Two related recombinases, XerC and XerD, belonging to the lambda integrase family of enzymes, are required for Xer site-specific recombination in vivo. In order to understand the roles of these proteins in the overall reaction mechanism, an in vitro recombination system using a synthetic Holliday junction-containing substrate has been developed ...
L K, Arciszewska, D J, Sherratt
openaire +2 more sources
A method for producing transgenic cells using a multi-integrase system on a human artificial chromosome vector. [PDF]
PLoS ONE, 2011 The production of cells capable of expressing gene(s) of interest is important for a variety of applications in biomedicine and biotechnology, including gene therapy and animal transgenesis.
Shigeyuki Yamaguchi +5 more
doaj +1 more source
Site-specific recombination in plane view [PDF]
Structure, 1997 Biochemists have worked long and hard on each reaction component and chemical step to reach the point of asking the question as to how protein and DNA molecules are arranged and rearranged in the process of site-specific recombination. The structures of several lambda integrase family members published recently have answered many of the questions about
Yang, Wei, Mizuuchi, Kiyoshi
openaire +2 more sources
The Site-Specific Recombination System of the Escherichia coli Bacteriophage Φ24B
Frontiers in Microbiology, 2020 Stx bacteriophages are members of the lambdoid group of phages and are responsible for Shiga toxin (Stx) production and the dissemination of Shiga toxin genes (stx) across shigatoxigenic Escherichia coli (STEC).
Mohammed Radhi Mohaisen +4 more
doaj +1 more source
An att site-based recombination reporter system for genome engineering and synthetic DNA assembly
BMC Biotechnology, 2017 Background Direct manipulation of the genome is a widespread technique for genetic studies and synthetic biology applications. The tyrosine and serine site-specific recombination systems of bacteriophages HK022 and ΦC31 are widely used for stable ...
Michael J. Bland +3 more
doaj +1 more source
Development of a novel in vitro assay for the evaluation of integron DNA integrase activity
Biotechnology & Biotechnological Equipment, 2016 Integrons play an important role in multidrug resistance. The integron platform codes for integrase (intI) that is required for gene cassette integration through site-specific recombination.
Fatemeh Tohidi +6 more
doaj +1 more source
Virulence, 2020 The proliferation of antibiotic resistance has its origins in horizontal gene transfer. The class 1 integrons mediate gene transfer by assimilating antibiotic-resistance genes through site-specific recombination.
Jun Zhang +8 more
doaj +1 more source