Bacteriophage lambda site-specific recombination. [PDF]
Mol MicrobiolAbstractThe site‐specific recombination pathway of bacteriophage λ encompasses isoenergetic but highly directional and tightly regulated integrative and excisive reactions that integrate and excise the vial chromosome into and out of the bacterial chromosome.
Van Duyne GD, Landy A.
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The integrase of genomic island GIsul2 mediates the mobilization of GIsul2 and ISCR-related element CR2-sul2 unit through site-specific recombination. [PDF]
Front Microbiol, 2022 In the worldwide health threat posed by antibiotic-resistant bacterial pathogens, mobile genetic elements (MGEs) play a critical role in favoring the dissemination of resistance genes.
Zhang G +10 more
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Large T antigen mediated target gene replication improves site-specific recombination efficiency. [PDF]
Front Bioeng BiotechnolWith advantages of high-fidelity, monoclonality and large cargo capacity, site-specific recombination (SSR) holds great promises for precise genomic modifications.
Wang Z, Chen C, Ge X.
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Site-specific recombination in plane view [PDF]
Structure, 1997 Biochemists have worked long and hard on each reaction component and chemical step to reach the point of asking the question as to how protein and DNA molecules are arranged and rearranged in the process of site-specific recombination. The structures of several lambda integrase family members published recently have answered many of the questions about
Yang, Wei, Mizuuchi, Kiyoshi
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Engineering integrative vectors based on phage site-specific recombination mechanism for Lactococcus lactis. [PDF]
BMC Biotechnol, 2019 Background Site-specific integration system allows foreign DNA to be integrated into the specific site of the host genome, enabling stable expression of heterologous protein.
Koko I +3 more
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Control of directionality in Streptomyces phage φBT1 integrase-mediated site-specific recombination. [PDF]
PLoS One, 2013 Streptomyces phage φBT1 integrates its genome into the attB site of the host chromosome with the attP site to generate attL and attR. The φBT1 integrase belongs to the large serine recombinase subfamily which directly binds to target sites to initiate ...
Zhang L, Zhu B, Dai R, Zhao G, Ding X.
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The λ Integrase Site-specific Recombination Pathway. [PDF]
Microbiol Spectr, 2015 ABSTRACTThe site-specific recombinase encoded by bacteriophage λ (Int) is responsible for integrating and excising the viral chromosome into and out of the chromosome of itsEscherichia colihost. Int carries out a reaction that is highly directional, tightly regulated, and depends upon an ensemble of accessory DNA bending proteins acting on 240 bp of ...
Landy A.
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Orthogonal LoxPsym sites allow multiplexed site-specific recombination in prokaryotic and eukaryotic hosts. [PDF]
Nat CommunSite-specific recombinases such as the Cre-LoxP system are routinely used for genome engineering in both prokaryotes and eukaryotes. Importantly, recombinases complement the CRISPR-Cas toolbox and provide the additional benefit of high-efficiency DNA ...
Cautereels C +9 more
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Utilization of Site-Specific Recombination in Biopharmaceutical Production. [PDF]
Iran Biomed J, 2016 Mammalian expression systems, due to their capacity in post-translational modification, are preferred systems for biopharmaceutical protein production. Several recombinant protein systems have been introduced to the market, most of which are under clinical development.
Ahmadi M +3 more
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Application of the Cre/loxP Site-Specific Recombination System for Gene Transformation in Aurantiochytrium limacinum. [PDF]
Molecules, 2015 The Cre/loxP site-specific recombination system was applied to Aurantiochytrium limacinum to obtain a transformant without the antibiotic resistance marker gene.
Sun H +8 more
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