Results 61 to 70 of about 10,065,751 (268)

Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining

Molecular Oncology, EarlyView.
IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis, Jack V. Mills, Wojciech Niedzwiedz, Valentine M. Macaulay   +3 more
wiley   +1 more source

Efficient Cre/loxP Site-Specific Recombination in a HepG2 Human Liver Cell Line

Cell Transplantation, 2000
A worldwide shortage of donor livers is a limiting factor of the clinical application of hepatocyte transplantation (HTX). To resolve this issue, we focused on a reversible immortalization system that allows temporary expansion of primary hepatocyte ...
Naoya Kobayashi M.D., Ph.D., Hirofumi Noguchi, Karen A. Westerman, Toshihisa Matsumura, Takamasa Watanabe, Toshinori Totsugawa, Toshiyoshi Fujiwara, Philippe Leboulch, Noriaki Tanaka   +8 more
doaj   +1 more source

Enhanced cell-permeant Cre protein for site-specific recombination in cultured cells

BMC Biotechnology, 2004
Background Cell-permeant Cre DNA site-specific recombinases provide an easily controlled means to regulate gene structure and function in living cells. Since recombination provides a stable and unambiguous record of protein uptake, the enzyme may also be
Ruley H Earl, Gebre-Amlak Kassatihun D, Jo Daewoong, Lin Qing   +3 more
doaj   +1 more source

Finding novel vulnerabilities of hypomorphic BRCA1 alleles

Molecular Oncology, EarlyView.
Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder, Klaas de Lint, Hồ Mỹ Phúc Võ, Mariana M. Góis, Rosalie A. Kampen, Marta San Martin Alonso, Ilse Nootenboom, Veronica Garzero, Tiemen J. Wendel, Rob M. F. Wolthuis, Sylvie M. Noordermeer   +10 more
wiley   +1 more source

Cell-Specific Ecdysone-Inducible Expression of FLP Recombinase in Mammalian Cells

BioTechniques, 1998
The ability of site-specific recombinases, like FLP and Cre, to catalyze alterations in genomic DNA is well established, whereas their application to genetic engineering strategies has been restricted because of the inability to temporally regulate their
Janet A. Sawicki, Bob Monks, Rebecca J. Morris   +2 more
doaj   +1 more source

Integrase-mediated recombination of the veb1 gene cassette encoding an extended-spectrum β-lactamase. [PDF]

PLoS ONE, 2012
The veb1 gene cassette encodes the extended spectrum β-lactamase, VEB-1 that is increasingly isolated from worldwide Gram-negative rods. Veb1 is commonly inserted into the variable region of different class 1 integrons in which it is always associated ...
Daniel Aubert, Thierry Naas, Patrice Nordmann   +2 more
doaj   +1 more source

Cancer‐associated mutations in endometriosis reframe a benign disease through molecular oncology

Molecular Oncology, EarlyView.
This review aims to comprehensively analyse cancer‐associated somatic mutations (CAMs) present in endometriotic lesions, emphasizing their biological roles, spatial distribution and implications for translational applications in medicine. By contextualizing a benign state within a genomic framework, this analysis seeks to establish its value as a ...
Clarissa Mujacic, Maria Concetta Vitale, Rita Siino, Emilia Di Giovanni, Carla Ferrante Bannera, Enrico Di Marco, Andrea Gottardo, Tancredi Didier Bazan Russo, Nadia Barraco, Alessandro Perez, Valerio Gristina, Antonio Galvano, Giuseppe Badalamenti, Antonio Russo, Viviana Bazan, Lorena Incorvaia   +15 more
wiley   +1 more source

Castration‐resistant prostate cancer cells are addicted to the high activity of cyclin‐dependent kinase 2

Molecular Oncology, EarlyView.
We show that emergence of castration‐resistant prostate (CRPC) is associated with significant upregulation of cyclins that positively regulate cyclin‐dependent kinase 2 (CDK2) and concomitant downregulation of CDK4 cyclins. This renders CRPC cells dependent on the high activity of CDK2, and CDK2 inhibitors synergistically sensitize CRPC cells to both ...
Joyeeta Chatterjee, Alessia Marin, Shivani Yalala, Harri M. Itkonen   +3 more
wiley   +1 more source

Bayesian modeling of recombination events in bacterial populations [PDF]

, 2008
Background: We consider the discovery of recombinant segments jointly with their origins within multilocus DNA sequences from bacteria representing heterogeneous populations of fairly closely related species.
Marttinen, P., Hanage William P, Mahenthiralingam Eshwar, Adam Baldwin, Jukka Corander, Marttinen Pekka, Hanage, William P., Pekka Marttinen, Hanage, W. P., Marttinen, Pekka, Dowson, Christopher G., Baldwin, A., Baldwin Adam, Dowson, C., Chris Dowson, Corander, J., Baldwin, Adam, Dowson Chris, William P Hanage, Eshwar Mahenthiralingam, Corander Jukka, Mahenthiralingam, Eshwar   +21 more
core   +1 more source

Differences in Integron Cassette Excision Dynamics Shape a Trade-Off between Evolvability and Genetic Capacitance

mBio, 2017
Integrons ensure a rapid and “on demand” response to environmental stresses driving bacterial adaptation. They are able to capture, store, and reorder functional gene cassettes due to site-specific recombination catalyzed by their integrase.
Céline Loot, Aleksandra Nivina, Jean Cury, José Antonio Escudero, Magaly Ducos-Galand, David Bikard, Eduardo P. C. Rocha, Didier Mazel   +7 more
doaj   +1 more source