Results 61 to 70 of about 283,041 (288)

TangleSolve: topological analysis of site-specific recombination

, 2002
Summary: TangleSolve is a program for analysing site-specific recombination using the tangle model. The program offers an easy-to-use graphical user interface and a visualization tool.
Mariel Vázquez, Yuki Saka
core   +1 more source

Exploring Protein Sequence Space Using Computationally Directed Recombination [PDF]

, 2006
Evolution has provided us with many protein sequences. However, these sequences represent a very small fraction of the possible sequences. In the laboratory, scientists have explored areas of sequence space not represented by natural proteins both to ...
Meyer, Michelle Margaret
core   +1 more source

The site-specific recombination system of actinophage TG1 [PDF]

FEMS Microbiology Letters, 2009
Actinophage TG1 forms stable lysogens by integrating at a unique site on chromosomes of Streptomyces strains. The phage (attP(TG1)) and bacterial (attB(TG1)) attachment sites for TG1 were deduced from comparative genomic studies on the TG1-lysogen and nonlysogen of Streptomyces avermitilis.
Kentaro, Morita, Tomoyuki, Yamamoto, Naoki, Fusada, Mamoru, Komatsu, Haruo, Ikeda, Nobutaka, Hirano, Hideo, Takahashi   +6 more
openaire   +2 more sources

Cell‐cycle‐specific lesion evolution rather than inhibition of double‐strand‐break repair underpins cisplatin radiosensitization

Molecular Oncology, EarlyView.
We analyze cisplatin–DNA adducts (CDAs) and double‐strand breaks (DSBs) in a cell‐cycle‐dependent manner. We find that CDAs form similarly across all cell cycle phases. DSBs arise only in S‐phase. CDAs might not directly impair DSB repair, but S‐phase DSB lesions evolve in the presence of CDAs and disrupt repair in G2, also causing radiosensitization ...
Ye Qiu, Xixi Lin, Emil Mladenov, Veronika Mladenova, Jürgen Thomale, Ali Sak, Yao Wang, Yunxuan Deng, Eleni Gkika, Martin Stuschke, George Iliakis   +10 more
wiley   +1 more source

The mechanism of site-specific recombination encoded by TN3 [PDF]

, 1984
The bacterial ampicillin resistance transposon Tn3 encodes a site-specific recombination system which mediates the second step in its transposition pathway: the resolution of cointegrates (Arthur and Sherratt (1979) Mol.gen.Genet. 175, 267-274). It vitro
Dyson, Paul J., Dyson, Paul J
core   +1 more source

Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining

Molecular Oncology, EarlyView.
IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis, Jack V. Mills, Wojciech Niedzwiedz, Valentine M. Macaulay   +3 more
wiley   +1 more source

Finding novel vulnerabilities of hypomorphic BRCA1 alleles

Molecular Oncology, EarlyView.
Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder, Klaas de Lint, Hồ Mỹ Phúc Võ, Mariana M. Góis, Rosalie A. Kampen, Marta San Martin Alonso, Ilse Nootenboom, Veronica Garzero, Tiemen J. Wendel, Rob M. F. Wolthuis, Sylvie M. Noordermeer   +10 more
wiley   +1 more source

Efficient Cre/loxP Site-Specific Recombination in a HepG2 Human Liver Cell Line

Cell Transplantation, 2000
A worldwide shortage of donor livers is a limiting factor of the clinical application of hepatocyte transplantation (HTX). To resolve this issue, we focused on a reversible immortalization system that allows temporary expansion of primary hepatocyte ...
Naoya Kobayashi M.D., Ph.D., Hirofumi Noguchi, Karen A. Westerman, Toshihisa Matsumura, Takamasa Watanabe, Toshinori Totsugawa, Toshiyoshi Fujiwara, Philippe Leboulch, Noriaki Tanaka   +8 more
doaj   +1 more source

Enhanced cell-permeant Cre protein for site-specific recombination in cultured cells

BMC Biotechnology, 2004
Background Cell-permeant Cre DNA site-specific recombinases provide an easily controlled means to regulate gene structure and function in living cells. Since recombination provides a stable and unambiguous record of protein uptake, the enzyme may also be
Ruley H Earl, Gebre-Amlak Kassatihun D, Jo Daewoong, Lin Qing   +3 more
doaj   +1 more source

Integrase-mediated recombination of the veb1 gene cassette encoding an extended-spectrum β-lactamase. [PDF]

PLoS ONE, 2012
The veb1 gene cassette encodes the extended spectrum β-lactamase, VEB-1 that is increasingly isolated from worldwide Gram-negative rods. Veb1 is commonly inserted into the variable region of different class 1 integrons in which it is always associated ...
Daniel Aubert, Thierry Naas, Patrice Nordmann   +2 more
doaj   +1 more source