Results 71 to 80 of about 758 (215)

Large T antigen mediated target gene replication improves site-specific recombination efficiency

Frontiers in Bioengineering and Biotechnology
With advantages of high-fidelity, monoclonality and large cargo capacity, site-specific recombination (SSR) holds great promises for precise genomic modifications.
Zening Wang, Zening Wang, Chuan Chen, Xin Ge, Xin Ge   +4 more
doaj   +1 more source

Bismuth‐Based Photocatalysts for Water Remediation: Design Strategies, Mechanisms, and Challenges

Advanced Functional Materials, EarlyView.
This review highlights representative design strategies for bismuth‐based semiconductor photocatalysts in solar‐driven water purification. Emphasis is placed on electronic structure regulation, oxygen‐vacancy engineering, and interfacial coupling to enable efficient full‐spectrum light utilization, improved charge separation, and selective oxidation in
Shan Liu, Yi Zhao, Liming Cai, Simin Long, Jing Kang, Jingkai Lin, Jimin Shen, Zhonglin Chen, Shaobin Wang, Huayang Zhang   +9 more
wiley   +1 more source

Catalytically Enhanced Near Room‐Temperature Hydrogen Sensing Using Pd‐ZnO Colloidal Crystals

Advanced Functional Materials, EarlyView.
Pd‐decorated ZnO colloidal crystal monolayers demonstrate excellent performance across a wide hydrogen concentration range at 33°C, with high selectivity and reproducible device fabrication. The sensors show strong responses even at low‐ppm H2 concentrations and an ultra‐low detection limit of 82 ppb, indicating their suitability for diverse hydrogen ...
Hamidah Alluhaybi, Samuel J. Ippolito, Chung Kim Nguyen, Durairaj Mahendiran, Michelle J. S. Spencer, Edwin L. H. Mayes, Billy James Murdoch, Jason Choi, Shamali De Silva, James Tardio, Adrian Trinchi, Ahmad E. Kandjani, Mohammad Al Kobaisi, Ylias Sabri   +13 more
wiley   +1 more source

Traceless and Site-Specific Ubiquitination of Recombinant Proteins

Journal of the American Chemical Society, 2011
Protein ubiquitination is a post-translational modification that regulates almost all aspects of eukaryotic biology. Here we discover the first routes for the efficient site-specific incorporation of δ-thiol-L-lysine (7) and δ-hydroxy-L-lysine (8) into recombinant proteins, via evolution of a pyrrolysyl-tRNA synthetase/tRNA(CUA) pair.
Virdee, Satpal, Kapadnis, Prashant B., Elliott, Thomas, Lang, Kathrin, Madrzak, Julia, Nguyen, Duy P., Riechmann, Lutz, Chin, Jason W.   +7 more
openaire   +3 more sources

Functionalizing Micro‐to‐Mesoscopic Electrode Architectures for Regulating Electron Transfer Behaviors in Electrocatalysis

Advanced Functional Materials, EarlyView.
A systematic review is conducted to assess the influence of electrode architecture across micro‐ to mesoscopic length scales on electron‐transfer pathways in electrocatalysis. We discuss the structure‐activity relationships in electrocatalytic applications, including resource recovery and environmental remediation, and provide cost‐effective, efficient
Manshu Zhao, Jiaojiao Zhang, Shideng Yuan, Xinmeng Yu, Keming Wan, Xiaoguang Duan, Zhining Wang   +6 more
wiley   +1 more source

Interaction‐Driven Assembly Pathways in Heterogeneous Active Microrobotic Systems

Advanced Functional Materials, EarlyView.
Peanut‐ and cube‐shaped light‐powered microrobots exhibit distinct magnetic and photocatalytic responses that determine their heterogeneous self‐assembly. In the dark, magnetic dipole interactions drive colloidal copolymerization with cubes acting as nucleation centers, whereas under light irradiation, phoretic interactions compete with magnetic ...
Domenico Calabrò, Roshan Velluvakandy, Martin Pumera, Francesco Priolo, Salvo Mirabella, Mario Urso   +5 more
wiley   +1 more source

Molecular Rotors: From Newtonian Fluids to Functional Materials as Viscosity and Temperature Sensing Tools

Advanced Functional Materials, EarlyView.
Molecular rotors are fluorescent molecules that are sensitive to viscosity and temperature variations in the environment. They exhibit a direct correlation between fluorescence and viscosity within Newtonian fluids. However, this correlation becomes less straightforward in complex systems.
Flavia Di Scala, Alice Briole, Valerii Myndrul, Thomas J. Cleij, Hanne Diliën, Bérengère Abou, Bart van Grinsven   +6 more
wiley   +1 more source

ORBIT: a New Paradigm for Genetic Engineering of Mycobacterial Chromosomes

mBio, 2018
Two efficient recombination systems were combined to produce a versatile method for chromosomal engineering that obviates the need to prepare double-stranded DNA (dsDNA) recombination substrates.
Kenan C. Murphy, Samantha J. Nelson, Subhalaxmi Nambi, Kadamba Papavinasasundaram, Christina E. Baer, Christopher M. Sassetti   +5 more
doaj   +1 more source

The λ Integrase Site-specific Recombination Pathway [PDF]

Microbiology Spectrum, 2015
ABSTRACTThe site-specific recombinase encoded by bacteriophage λ (Int) is responsible for integrating and excising the viral chromosome into and out of the chromosome of itsEscherichia colihost. Int carries out a reaction that is highly directional, tightly regulated, and depends upon an ensemble of accessory DNA bending proteins acting on 240 bp of ...
openaire   +2 more sources

Intrinsic Interfacial Energy Alignment Enables ETL‐Free Organic Solar Cells

Advanced Functional Materials, EarlyView.
Molecular orientation of the small‐molecule donor BTR‐Cl induces a vacuum‐level shift that pins the Fermi level at ∼4.4 eV at the active layer/Ag interface, enabling Ohmic contact formation without an electron transport layer. This strategy is extended to ternary PM6:Y6:BTR‐Cl devices, achieving an ETL‐free efficiency of 18.6% with enhanced operational
Heng Liu, Yuang Fu, Cangyu Wang, Luhang Xu, Xian‐Kai Chen, Man Chung Tang, Xinhui Lu   +6 more
wiley   +1 more source