Results 81 to 90 of about 283,041 (288)

The unconventional Xer recombination machinery of Streptococci/Lactococci.

open access: yesPLoS Genetics, 2007
Homologous recombination between circular sister chromosomes during DNA replication in bacteria can generate chromosome dimers that must be resolved into monomers prior to cell division.
Pascal Le Bourgeois   +8 more
doaj   +1 more source

Microcin H47 system: an Escherichia coli small genomic island with novel features. [PDF]

open access: yesPLoS ONE, 2011
Genomic islands are DNA regions containing variable genetic information related to secondary metabolism. Frequently, they have the ability to excise from and integrate into replicons through site-specific recombination.
María F Azpiroz   +2 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

open access: yesFEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova   +2 more
wiley   +1 more source

Analysis of xer Site-Specific Recombination In Vivo [PDF]

open access: yes, 1992
The xer site-specific recombination system is employed in two related biological processes. The first of these roles is in resolving multimers of high copy number, randomly partitioned plasmids in order to ensure their heritable stability.
McCulloch, Richard
core  

Topological selectivity in Xer site-specific recombination.

open access: yes, 1997
The product topology of Xer-mediated site-specific recombination at plasmid sites has been determined. The product of deletion at pSC101 psi is a right-handed antiparallel 4-noded catenane. The ColE1 cer deletion product has an identical topology, except
Bath, J   +6 more
core   +1 more source

Comparative analysis of right element mutant lox sites on recombination efficiency in embryonic stem cells

open access: yesBMC Biotechnology, 2010
Background Cre-mediated site-specific integrative recombination in mouse embryonic stem (ES) cells is a useful tool for genome engineering, allowing precise and repeated site-specific integration. To promote the integrative reaction, a left element/right
Araki Masatake   +3 more
doaj   +1 more source

Interfacial Modulation for High‐Efficiency Large‐Area Organic Photovoltaics and Perovskite‐Organic Tandem Solar Modules

open access: yesAdvanced Functional Materials, EarlyView.
Self‐assembled monolayers (SAMs) are promising hole‐transporting materials for organic photovoltaics (OPVs), but suffer from self‐aggregation and poor large‐area uniformity. We find that interfacial modification using nicotinic hydrazide can eliminate the residual SAM aggregates by forming energetically favorable complexes, yielding uniform SAM.
Seongwon Yoon   +10 more
wiley   +1 more source

The Role of Ultra‐Thin Buffer Layers for Achieving Ultra‐Low Dark Currents in Single‐Component Organic Photodetectors

open access: yesAdvanced Functional Materials, EarlyView.
Here, SubNc single‐component organic photodiodes (SC‐OPDs) are investigated, which achieve highly competitive performance metrics, including high EQE, ultra‐low JD, and high specific detectivity (D*). This study emphasizes the critical role of an organic buffer layer in studying the interface energetics and effects to achieve state‐of‐the‐art ...
Anncharlott Kusber   +12 more
wiley   +1 more source

Structural snapshots of Xer recombination reveal activation by synaptic complex remodeling and DNA bending

open access: yeseLife, 2016
Bacterial Xer site-specific recombinases play an essential genome maintenance role by unlinking chromosome multimers, but their mechanism of action has remained structurally uncharacterized. Here, we present two high-resolution structures of Helicobacter
Aleksandra Bebel   +4 more
doaj   +1 more source

Homogenous FACsPbI3 Perovskite Solar Cells Enabled by a Seed‐Facilitated Cation Exchange Strategy

open access: yesAdvanced Functional Materials, EarlyView.
A novel seed‐facilitated cation exchange strategy is designed for homogenous FACsPbI3 perovskites using the biomass‐derived solvent γ‐valerolactone. Pre‐deposited 0D Cs4PbI6 seeds induce rapid nucleation and efficient cation exchange, producing a uniform FA–Cs distribution and significantly reducing defect density, enabling high‐performance and stable ...
Meng Ren   +10 more
wiley   +1 more source

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