Size-exclusion chromatography-based enrichment of extracellular vesicles from urine samples [PDF]
Journal of Extracellular Vesicles, 2015 Renal biopsy is the gold-standard procedure to diagnose most of renal pathologies. However, this invasive method is of limited repeatability and often describes an irreversible renal damage.
Inés Lozano-Ramos +7 more
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Enrichment of extracellular vesicles from human synovial fluid using size exclusion chromatography
Journal of Extracellular Vesicles, 2018 As a complex biological fluid, human synovial fluid (SF) presents challenges for extracellular vesicle (EV) enrichment using standard methods. In this study of human SF, a size exclusion chromatography (SEC)-based method of EV enrichment is shown to ...
Andrew D. Foers +8 more
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Partial Purification of Peroxiredoxin-2 From Porcine Skeletal Muscle
Meat and Muscle Biology, 2021 Fresh meat quality is adversely affected by protein oxidation. However, a fundamental understanding of the diverse factors that influence protein oxidation in postmortem muscle remains elusive.
Edward M;. Steadham +3 more
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Characterization of hemodialysis membranes by inverse size exclusion chromatography [PDF]
, 1995 Inverse size exclusion chromatography (i-SEC) was used to characterize three different cellulosic hollow fiber hemodialysis membranes, i.e. low-flux cuprophan and hemophan and high-flux RC-HP400A.
Bargeman, Derk +5 more
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Size-exclusion chromatographic NMR of polymer mixtures [PDF]
, 2014 The use of chromatographic stationary phases or solvent modifiers to modulate diffusion properties in NMR experiments is now well established. Their use can be to improve resolution in the diffusion domain or to provide an insight into analyte–modifier ...
Adams +32 more
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Purification of bacteriocins using size-exclusion chromatography
Bangladesh Journal of Pharmacology, 2016 The bacteriocin purification involves following main steps. a). Extraction of cell-free-supernatant of bacteria. b). Ammonium sulfate precipitation. c). Dialysis. d). Diafiltration using PVP and e). Size-exclusion chromatography.
Vivek K. Bajpai +2 more
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Calibration of ultrafiltration membranes against size exclusion chromatography columns [PDF]
, 2010 Using the extension of the concept of universal calibration parameter, yielding a relation between the hydrodynamic volume of molecules and the elution volume in sizeexclusionchromatography (SEC), to retention coefficients in ultrafiltration (UF), we ...
Aimar, Pierre, Meireles, Martine
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Size exclusion chromatography for extraction of serum bile acids.
Journal of Lipid Research, 1990 A major problem in the measurement of serum bile acids is their quantitative extraction from the high molecular protein matrix. In our hands, the standard techniques of adsorption and reversed-phase chromatography have yielded incomplete recovery for ...
R Nuber, H Maucher, EF Stange
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Size exclusion chromatography as green support for forced degradation study of adalimumab
Acta Pharmaceutica, 2023 Size exclusion chromatography (SEC) has become a powerful tool for analysing size variants of biologic drugs in their native form. Modern SEC can be defined by the use of chromatographic columns packed with sub-3 µm particles, allowing an increase in ...
Kovačić Jelena +3 more
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Uncertainty of Size-Exclusion Chromatography Method in Quality Control of Bevacizumab Batches
Separations, 2021 In addition to the analytical challenges related to the size and complexity of biopharmaceutical drugs, the inherent variability that arises due to their manufacturing process requires monitoring throughout the production process to ensure the safety and
Alexis Oliva, Matías Llabrés
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