Results 31 to 40 of about 192,952 (293)

Combination of Size-Exclusion Chromatography and Ultracentrifugation Improves the Proteomic Profiling of Plasma-Derived Small Extracellular Vesicles

open access: yesBiological Procedures Online, 2020
Background Circulating small extracellular vesicles (sEVs) and its associated proteins are of great interest in the early detection of many diseases. However, there is no gold standard for plasma sEVs isolation, especially for proteomic profiling which ...
Rui Wei   +6 more
doaj   +1 more source

Purification and size exclusion chromatography of PaoD. [PDF]

open access: yes, 2014
(A) 12% SDS/PAGE of the purified protein after Ni-TED chromatography. (B) Size exclusion chromatography of PaoD. Freshly purified PaoD was analyzed by analytical size exclusion chromatography in 50 mM Tris-HCl, 300 mM NaCl, pH 8.0 using a Superdex 75 ...
Eurico J. Cabrita (518149)   +6 more
core   +1 more source

Size Exclusion Chromatography analyses of CwlD variants. [PDF]

open access: yes, 2021
Purified His-tagged CwlDWT, CwlDH49A, and CwlDE65A were analyzed by size exclusion chromatography. mAU corresponds to the UV absorbance measurements (A280) during the protein elution. (TIF)
Sylvie Doublié (280774)   +4 more
core   +1 more source

Separation of whey proteins for chromatography liquid

open access: yesScientia Agropecuaria, 2010
This paper describes and compares three chromatographic methods for the analysis and quantification of most abundant proteins in cheese whey, -lactalbumin and -lactoglobulin. The methods were: Reverse-phase high performance liquid chromatography, anion
Abraham D. Giraldo Zuñiga   +3 more
doaj   +1 more source

Quantitative Analysis of Step-Growth Polymers by Size Exclusion Chromatography [PDF]

open access: yes, 2016
We report an advanced analysis protocol that allows to quantitatively study the course of step-growth reactions by size exclusion chromatography on the example of the depolymerization of a Diels-Alder polymer based on a furane/maleimide couple at ...
Lederer, Albena   +15 more
core   +1 more source

Improving size-exclusion chromatography separation for glycogen [PDF]

open access: yes, 2014
Exosomes are a subset of extracellular vesicles (EVs) that have important roles in intercellular communication. They contain and carry bioactive molecules within their membranes which are delivered to target cells.
Powell, Prudence O.   +6 more
core   +1 more source

Framework for rapid comparison of extracellular vesicle isolation methods

open access: yeseLife, 2021
Extracellular vesicles (EVs) are released by all cells into biofluids and hold great promise as reservoirs of disease biomarkers. One of the main challenges in studying EVs is a lack of methods to quantify EVs that are sensitive enough and can ...
Dmitry Ter-Ovanesyan   +6 more
doaj   +1 more source

Molecular Dispersion of Starch as a Crucial Parameter during Size-Exclusion Chromatography

open access: yesFoods, 2020
Starch, α-polyglucan consisting of a large number of anhydroglucose units joined by α-1,4- and α-1,6-glycosidic bonds, seems to be characterized by a simple structure when compared to other natural polymers.
Artur Szwengiel, Piotr Kubiak
doaj   +1 more source

Investigation and interpretation of band broadening in size exclusion chromatography [PDF]

open access: yes, 2019
Study of Band Broadening occurring in Size Exclusion Chromatography (SEC) is reported using very narrow PS standards obtained and characterised by Temperature Gradient Interaction Chromatography (TGIC).
Chang, T   +4 more
core   +1 more source

Isolation of Extracellular Vesicles from Human Follicular Fluid: Size-Exclusion Chromatography versus Ultracentrifugation

open access: yesBiomolecules, 2023
Follicular fluid (FF) is the microenvironment where a growing oocyte develops. Intrafollicular communication ensures oocyte competence and is carried out through paracrine signaling, the exchange of molecules via gap junctions, and the trafficking of ...
Maria Soares   +7 more
doaj   +1 more source

Home - About - Disclaimer - Privacy