High-grade extracellular vesicles preparation by combined size-exclusion and affinity chromatography
Scientific Reports, 2021 Extracellular vesicles (EVs) have recently gained growing interest for their diagnostic and therapeutic potential. Despite this, few protocols have been reported for the isolation of EVs with preserved biological function.
Cristina Bellotti +4 more
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Separation of whey proteins for chromatography liquid
Scientia Agropecuaria, 2010 This paper describes and compares three chromatographic methods for the analysis and quantification of most abundant proteins in cheese whey, -lactalbumin and -lactoglobulin. The methods were: Reverse-phase high performance liquid chromatography, anion
Abraham D. Giraldo Zuñiga +3 more
doaj +1 more source
Size fractionation by slalom chromatography and hydrodynamic chromatography [PDF]
, 2008 Hydrodynamic chromatography, also called separation by flow, is based on the use of the parabolic flow profile occurring in open capillaries or in the pores from a column filled with non-porous particles. The hydrodynamic chromatography separation medium,
Dias, Ricardo P.
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Molecular Dispersion of Starch as a Crucial Parameter during Size-Exclusion Chromatography
Foods, 2020 Starch, α-polyglucan consisting of a large number of anhydroglucose units joined by α-1,4- and α-1,6-glycosidic bonds, seems to be characterized by a simple structure when compared to other natural polymers.
Artur Szwengiel, Piotr Kubiak
doaj +1 more source
Effect of cytostatic drug presence on extracellular polymeric substances formation in municipal wastewater treated by membrane bioreactor [PDF]
, 2010 The effect of the cyclophosphamide and its mean metabolites on extracellular polymeric substances (EPS) formation was investigated. Two lab-scale membrane bioreactors were followed in parallel (one with the cytostatic drugs, the second without ...
Albasi, Claire +5 more
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Framework for rapid comparison of extracellular vesicle isolation methods
eLife, 2021 Extracellular vesicles (EVs) are released by all cells into biofluids and hold great promise as reservoirs of disease biomarkers. One of the main challenges in studying EVs is a lack of methods to quantify EVs that are sensitive enough and can ...
Dmitry Ter-Ovanesyan +6 more
doaj +1 more source
Iron-based ferritin nanocore as a contrast agent [PDF]
, 2010 Self-assembling protein cages have been exploited as templates for nanoparticle synthesis. The ferritin molecule, a protein cage present in most living systems, stores excess soluble ferrous iron in the form of an insoluble ferric complex within its ...
Johnson, Eric +4 more
core +2 more sources
Biomolecules, 2023 Follicular fluid (FF) is the microenvironment where a growing oocyte develops. Intrafollicular communication ensures oocyte competence and is carried out through paracrine signaling, the exchange of molecules via gap junctions, and the trafficking of ...
Maria Soares +7 more
doaj +1 more source
A high-affinity antibody against the CSP N-terminal domain lacks Plasmodium falciparum inhibitory activity [PDF]
, 2020 Malaria is a global health concern and research efforts are ongoing to develop a superior vaccine to RTS,S/AS01. To guide immunogen design, we seek a comprehensive understanding of the protective humoral response against Plasmodium falciparum ...
Bosch, A. +15 more
core +2 more sources
ABA triblock copolymers: from controlled synthesis to controlled function [PDF]
, 2003 The ABA amphiphilic block copolymers, poly(hydroxyethyl methacrylate-hlock-methylphenylsilane-block-hydroxyethyl methacrylate) (PHEMA-PMPS-PHEMA) and poly[oligo(ethylene glycol) methyl ether methacrylate-block-methylphenylsilane-block-oligo(ethylene ...
Allen +71 more
core +6 more sources