Viruses, 2021 Extracellular vesicles (EVs) are cell-derived membranous particles secreted by all cell types (including virus infected and uninfected cells) into the extracellular milieu.
Hussein Kaddour +2 more
semanticscholar +3 more sources
Single-step isolation of extracellular vesicles by size-exclusion chromatography [PDF]
Journal of Extracellular Vesicles, 2014 Background: Isolation of extracellular vesicles from plasma is a challenge due to the presence of proteins and lipoproteins. Isolation of vesicles using differential centrifugation or density-gradient ultracentrifugation results in co-isolation of ...
Anita E. Grootemaat +5 more
core +3 more sources
Optimization of extracellular vesicle isolation and their separation from lipoproteins by size exclusion chromatography [PDF]
J Extracell Biol, 2023 Interest in the use of extracellular vesicles (EVs) as biomarkers of disease is rapidly growing. However, one main unsolved issue in the EV field is finding a technique able to eliminate non‐EV contaminants present in biofluid samples in a one‐step ...
Beatriz Benayas +4 more
semanticscholar +2 more sources
Size‐exclusion chromatography‐based enrichment of extracellular vesicles from urine samples [PDF]
Journal of Extracellular Vesicles, 2015 Renal biopsy is the gold-standard procedure to diagnose most of renal pathologies. However, this invasive method is of limited repeatability and often describes an irreversible renal damage.
Inés Lozano‐Ramos +7 more
openalex +3 more sources
High-grade extracellular vesicles preparation by combined size-exclusion and affinity chromatography
Scientific Reports, 2021 Extracellular vesicles (EVs) have recently gained growing interest for their diagnostic and therapeutic potential. Despite this, few protocols have been reported for the isolation of EVs with preserved biological function.
Cristina Bellotti +4 more
semanticscholar +2 more sources
Biological Procedures Online, 2020 Background Circulating small extracellular vesicles (sEVs) and its associated proteins are of great interest in the early detection of many diseases. However, there is no gold standard for plasma sEVs isolation, especially for proteomic profiling which ...
Rui Wei +6 more
semanticscholar +3 more sources
Comparative Proteomics of Seminal Exosomes Reveals Size-Exclusion Chromatography Outperforms Ultracentrifugation [PDF]
BiomedicinesBackground: Extracellular vesicles, particularly exosomes, play a crucial role in cell–cell communication and as carriers of biomarkers. However, their use in clinical settings is limited due to a lack of standardized isolation and characterization ...
Ajaya K. Moharana +6 more
doaj +2 more sources
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data [PDF]
Crystals, 2020 We describe the updated size-exclusion chromatography small angle X-ray scattering (SEC-SAXS) set-up used at the P12 bioSAXS beam line of the European Molecular Biology Laboratory (EMBL) at the PETRAIII synchrotron, DESY Hamburg (Germany).
Melissa A. Graewert +6 more
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Size Exclusion Chromatography–Mass Photometry: A New Method for Adeno-Associated Virus Product Characterization [PDF]
Cells, 2023 Over the past decade, adeno-associated viruses (AAVs) have attained significant prominence in gene therapy and genome editing applications, necessitating the development of robust and precise methodologies to ensure the quality and purity of AAV products.
Di Wu +3 more
doaj +2 more sources
Determination of Hydrodynamic Radius of Proteins by Size Exclusion Chromatography.
Bio-Protocol, 2017 Size exclusion chromatography (SEC) or gel filtration is a hydrodynamic technique that separates molecules in solution as a function of their size and shape. In the case of proteins, the hydrodynamic value that can be experimentally derived is the Stokes
V. La Verde, P. Dominici, A. Astegno
semanticscholar +3 more sources