Results 61 to 70 of about 5,377 (266)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Sliding-Mode Observer for Speed and Position Sensorless Control of Linear-PMSM

Electrical, Control and Communication Engineering, 2014
The paper presents a sliding-mode observer that utilizes sigmoid function for speed and position sensorless control of permanent-magnet linear synchronous motor (PMLSM). In conventional sliding mode observer method there are the chattering phenomenon and
Kazraji Saeed Masoumi, Soflayi Ramin Bavili, Sharifian Mohammad Bagher Bannae   +2 more
doaj   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Hydrostatic pressure activates HIF‐1α via β‐catenin to promote stemness in breast cancer cells

FEBS Open Bio, EarlyView.
To mimic the elevated intestinal fluid pressure in breast cancers, we loaded human breast cancer cells (MCF‐7, MDA‐MB‐453, and BT‐474) to 50 mmHg hydrostatic pressure. Hydrostatic pressure exposure upregulated HIF‐1α and induced stemness in MCF‐7 and BT‐474 cells.
Da Zhai, Yong Xu, Jingjing Ma, Ratchanee Duangrat, Chen Yan, Jingyan Liang, Hua Wei, Bin Jiang, Tao‐Sheng Li   +8 more
wiley   +1 more source

Observer–Based Sliding–Mode Fault–Tolerant Consistent Control for Hybrid Event–Triggered Multi–Agent Systems

International Journal of Applied Mathematics and Computer Science
An observer-based hybrid event-triggered sliding mode fault-tolerant consistent control strategy is proposed for actuator faults in nonlinear second-order leader–follower multi-agent systems.
Xia Dexian, Fu Xingjian
doaj   +1 more source

State of Charge Estimation for Power Lithium-Ion Battery Using a Fuzzy Logic Sliding Mode Observer

Energies, 2019
State of charge (SOC) estimation is of vital importance for the battery management system in electric vehicles. This paper proposes a new fuzzy logic sliding mode observer for SOC estimation.
Wenhui Zheng, Bizhong Xia, Wei Wang, Yongzhi Lai, Mingwang Wang, Huawen Wang   +5 more
doaj   +1 more source

Adaptive Backstepping Sliding Mode Control of the Hybrid Conveying Mechanism with Mismatched Disturbances via Nonlinear Disturbance Observers

Journal of Control Science and Engineering, 2020
An adaptive backstepping sliding mode controller combined with a nonlinear disturbance observer is designed for trajectory tracking of the electrically driven hybrid conveying mechanism with mismatched disturbances.
Wei Yuan, Guoqin Gao, Jianzhen Li
doaj   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Sensorless Fault-Tolerant Control Strategy of Six-Phase Induction Machine Based on Harmonic Suppression and Sliding Mode Observer

IEEE Access, 2019
This paper deduces a unified mathematical model of open-circuit fault of six-phase induction machine, and purposes a fault-tolerence sensorless strategy based on the sliding mode observer(SMO) to estimate the rotor flux and rotor speed.
Yiwen Geng, Zou Lai, Yonggang Li, Ding Wang, Ruicheng Chen, Pengfei Zheng   +5 more
doaj   +1 more source