Contribution of smFRET to Chromatin Research
Biophysica, 2023 Chromatins are structural components of chromosomes and consist of DNA and histone proteins. The structure, dynamics, and function of chromatins are important in regulating genetic processes. Several different experimental and theoretical tools have been
Bhaswati Sengupta, Mai Huynh
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A DNA Scaffold Approach Facilitates 5' Labeling of the SARS-CoV-2 RNA Pseudoknot for Single-Molecule Förster Resonance Energy Transfer Investigation. [PDF]
ChemphyschemSingle-molecule Förster resonance energy transfer (smFRET) studies of highly structured RNA molecules are often frustrated by issues with efficient dye conjugation.
Graham SP +5 more
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Biophysica, 2022 Single-molecule Förster resonance energy transfer (smFRET) enables the deconvolution of various conformational substates of biomolecules. Over the past two decades, it has been widely used to understand the conformational dynamics of enzymes.
Mai Huynh, Bhaswati Sengupta
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In-cell single-molecule FRET measurement of cytosolic RAF proteins to investigate the structural states and kinetics among them [PDF]
Frontiers in Molecular BiosciencesThe structure of a protein is closely linked to its function. Many proteins undergo conformational changes while working in living cells. Consequently, proteins in various structural states coexist in the native cellular environment.
Kenji Okamoto, Yasushi Sako
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Single-photon smFRET. I: Theory and conceptual basis
Biophysical Reports, 2023 We present a unified conceptual framework and the associated software package for single-molecule Förster resonance energy transfer (smFRET) analysis from single-photon arrivals leveraging Bayesian nonparametrics, BNP-FRET.
Ayush Saurabh +4 more
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Single-photon smFRET: II. Application to continuous illumination
Biophysical Reports, 2023 Here we adapt the Bayesian nonparametrics (BNP) framework presented in the first companion article to analyze kinetics from single-photon, single-molecule Förster resonance energy transfer (smFRET) traces generated under continuous illumination.
Ayush Saurabh +4 more
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Multiplexed smFRET Nucleic Acid Sensing Using DNA Nanotweezers
Biosensors, 2023 The multiplexed detection of disease biomarkers is part of an ongoing effort toward improving the quality of diagnostic testing, reducing the cost of analysis, and accelerating the treatment processes.
Anisa Kaur +4 more
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Developing Multichannel smFRET Approach to Dissecting Ribosomal Mechanisms
Chemical & Biomedical ImagingThe ribosome, a 2.6 megadalton biomolecule measuring approximately 20 nm in diameter, coordinates numerous ligands, factors, and regulators to translate proteins with high fidelity and speed.
Ran Lin, Yuhong Wang
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Crowding-induced morphological changes in synthetic lipid vesicles determined using smFRET
Frontiers in Bioengineering and Biotechnology, 2022 Lipid vesicles are valuable mesoscale molecular confinement vessels for studying membrane mechanics and lipid–protein interactions, and they have found utility among bio-inspired technologies, including drug delivery vehicles.
Steven D. Quinn +9 more
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An smFRET assay to probe the impact of antibiotics on intersubunit rotation in eukaryotic ribosomes. [PDF]
Methods EnzymolGrove AK +4 more
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