Results 121 to 130 of about 6,254 (216)
Chemistry – A European Journal, Volume 31, Issue 32, June 6, 2025.Time‐resolved nuclear magnetic resonance (NMR) and circular dichroism (CD) spectroscopy were utilized to investigate the complex G‐quadruplex folding landscape of the human telomeric DNA in the presence of the ligand Phen‐DC3. Our findings revealed significant differences in the kinetics of the pathways originating from the unfolded or K+‐bound state ...
Ines Burkhart +3 more
wiley +1 more source
Automated smFRET microscope for the quantification of label-free DNA oligos
Biomedical Optics Express, 2019 Single-molecule FRET (smFRET) spectroscopy is a powerful tool for studying inhomogeneous dynamics in biological systems. However, because of the intrinsic variations that accompany the sample sizes, massive data sets are essential to extract statistically reliable information.
Ran Lin, Yuhong Wang
openaire +2 more sources
Conformational changes in Arp2/3 complex induced by ATP, WASp-VCA, and actin filaments [PDF]
, 2018 We used fluorescence spectroscopy and EM to determine how binding of ATP, nucleation-promoting factors, actin monomers, and actin filaments changes the conformation of Arp2/3 complex during the process that nucleates an actin filament branch.
Chou, Steven Z. +4 more
core +1 more source
CHIMIA, 2013 Single-molecule spectroscopy allows the direct observation of conformational dynamics in individual biomolecules. Here, we describe how single-molecule Förster resonance energy transfer (smFRET) reveals heterogeneous kinetics in the EBS1*/IBS1 ...
Sebastian L. B. König +2 more
doaj +1 more source
Conformational transition of FGFR kinase activation revealed by site-specific unnatural amino acid reporter and single molecule FRET [PDF]
, 2017 Protein kinases share significant structural similarity; however, structural features alone are insufficient to explain their diverse functions. Thus, bridging the gap between static structure and function requires a more detailed understanding of ...
Alexandrov, Y +10 more
core
eIF4G stimulates the activity of the DEAD box protein eIF4A by a conformational guidance mechanism [PDF]
, 2017 The activity of eIF4A, a key player in translation initiation, is regulated by other translation factors through currently unknown mechanisms. Here, we provide the necessary framework to understand the mechanism of eIF4A's regulation by eIF4G.
Gubaev, Airat +3 more
core
Probing Allostery in Oligomeric M2 Receptors using smFRET [PDF]
Biophysical Journal, 2021 Dennis D. Fernandes +2 more
openaire +1 more source
An optimization algorithm for single-molecule fluorescence resonance (smFRET) data processing
Acta Physica Sinica, 2017 The single-molecule fluorescence resonance energy transfer (smFRET) technique plays an important role in the development of biophysics. Measuring the changes of the fluorescence intensities of donor and acceptor and of the FRET efficiency can reveal the changes of distance between the labeling positions.
null Lü Xi-Ming +7 more
openaire +1 more source
Viruses, 2018 Viruses are simple agents exhibiting complex reproductive mechanisms. Decades of research have provided crucial basic insights, antiviral medication and moderately successful gene therapy trials. The most infectious viral particle is, however, not always
Nagma Parveen +3 more
doaj +1 more source
The Spliceosomal Protein Prp8 Stabilizes A Compact Conformation Of The U2-U6 Complex [PDF]
, 2012 The spliceosome is a large, RNA-protein complex that catalyzes pre-mRNA splicing during mRNA maturation. The RNA components (small nuclear RNA; snRNAs) of the spliceosome have been well studied and are believed to be involved in the splicing catalysis ...
Subasinghe, Subasinghe Appuhamilage Lemintha Imali
core +1 more source