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Single-molecule visualization of ATP-induced dynamics of the subunit composition of an ECF transporter complex under turnover conditions. [PDF]
Nat CommunLefebvre SN +3 more
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smFRET-assisted RNA structure prediction
Communications in Information and SystemsSingle-molecule Förster Resonance Energy Transfer (smFRET) is a powerful biophysical technique that utilizes the distance-dependent energy transfer between donor and acceptor dyes linked to individual molecules, providing insights into molecular ...
Jun Li, Nils G. Walter, Shi-Jie Chen
semanticscholar +3 more sources
Observing transmembrane conformational signaling with smFRET.
Biophysical Journal, 2023G. Schlau-Cohen +2 more
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smFRET study of rRNA dimerization at the peptidyl transfer center
Biophysical Chemistry, 2021The ribosome is a ribozyme. At the peptidyl transfer center (PTC) of 180 nt, two loops (the A- and P- loops) bind to tRNAs and position them in close proximity for efficient peptidyl ligation. There is also a 2-fold rotational symmetry in the PTC, which suggests that the precursor of the modern ribosome possibly emerged through dimerization and gene ...
Doris, Xu, Yuhong, Wang
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BPS2025 - SmFRET investigation of structural dynamics during T-loop formation
Biophysical JournalArianna N Lacen, Hui-Ting Lee
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Journal of Physical Chemistry B, 2023
Many biological molecules respond to external stimuli that can cause their conformational states to shift from one steady state to another. Single-molecule FRET (Fluorescence Resonance Energy Transfer) is of particular interest to not only define the ...
Ting Chen, Fengnan Gao, Yan-Wen Tan
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Many biological molecules respond to external stimuli that can cause their conformational states to shift from one steady state to another. Single-molecule FRET (Fluorescence Resonance Energy Transfer) is of particular interest to not only define the ...
Ting Chen, Fengnan Gao, Yan-Wen Tan
semanticscholar +1 more source
Elucidating the conformational dynamics of human Hsp90 using smFRET
Biophysical JournalEcenaz Bilgen +4 more
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A Two-Step Method for smFRET Data Analysis
The Journal of Physical Chemistry B, 2016We demonstrate a two-step data analysis method to increase the accuracy of single-molecule Förster Resonance Energy Transfer (smFRET) experiments. Most current smFRET studies are at a time resolution on the millisecond level. When the system also contains molecular dynamics on the millisecond level, simulations show that large errors are present (e.g.,
Jixin, Chen +4 more
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