Results 11 to 20 of about 6,254 (216)
Single-photon smFRET. III. Application to pulsed illumination
Biophysical Reports, 2022 Förster resonance energy transfer (FRET) using pulsed illumination has been pivotal in leveraging lifetime information in FRET analysis. However, there remain major challenges in quantitative single photon, single molecule FRET (smFRET) data analysis ...
Matthew Safar +7 more
semanticscholar +3 more sources
Learning Continuous Potentials from smFRET
Biophysical Journal, 2022 Potential energy landscapes are useful models in describing events such as protein folding and binding. While single molecule fluorescence resonance energy transfer (smFRET) experiments encode information on continuous potentials for the system probed ...
J. Shepard, IV Bryan, S. Pressé
semanticscholar +3 more sources
Recovering true FRET efficiencies from smFRET investigations requires triplet state mitigation
Nature MethodsSingle-molecule fluorescence resonance energy transfer (smFRET) methods employed to quantify time-dependent compositional and conformational changes within biomolecules require elevated illumination intensities to recover robust photon emission streams ...
Avik K. Pati +9 more
semanticscholar +3 more sources
Multi-channel smFRET study reveals a compact conformation of EF-G on the ribosome
The International Journal of Biochemistry & Cell BiologyWhile elongation factor G (EF-G) is crucial for ribosome translocation, the role of its GTP hydrolysis remains ambiguous. EF-G’s indispensability is further exemplified by the phosphorylation of human eukaryotic elongation factor 2 (eEF2) at Thr56, which
Jordan L. Johnson +5 more
semanticscholar +5 more sources
Observation of SAM-VI Riboswitch Dynamics Using Single-Molecule FRET [PDF]
BiomoleculesRiboswitches regulate gene expression through intricate dynamic conformational transitions, with divalent cation Mg2+ and their ligands playing pivotal roles in this process.
Yanyan Xue +4 more
doaj +2 more sources
Single-Molecule FRET-Tracking of InlB-Activated MET Receptors in Living Cells. [PDF]
SmallSingle‐molecule FRET and single‐particle tracking reveal how ligand binding drives dimerization and activation of the MET receptor tyrosine kinase in living cells. Single‐molecule FRET reports on the lifetime of the (MET:InlB)2 complex. Distinct diffusion coefficients and modes of monomeric and dimeric receptor complexes uncover nanometer‐scale ...
Li Y +4 more
europepmc +2 more sources
40S scanning unveiled by smFRET
Biophysical Journal, 2022 Jinfan Wang, J. Puglisi
semanticscholar +2 more sources
Micromachines, 2021 Single-molecule Förster resonance energy transfer (smFRET) inherits the strategy of measurement from the effective “spectroscopic ruler” FRET and can be utilized to observe molecular behaviors with relatively high throughput at nanometer scale.
Yi Qiao +4 more
doaj +1 more source
Extraction of rapid kinetics from smFRET measurements using integrative detectors
Cell Reports Physical Science, 2021 Hidden Markov models (HMMs) are used to learn single-molecule kinetics across a range of experimental techniques. By their construction, HMMs assume that single-molecule events occur on slower timescales than those of data acquisition. To move beyond that HMM limitation and allow for single-molecule events to occur on any timescale, we must treat ...
Z. Kilic +5 more
semanticscholar +3 more sources
eLife, 2021 Single-molecule FRET (smFRET) has become a mainstream technique for studying biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by an ever-increasing number of groups has generated significant progress in sample ...
Eitan Lerner +45 more
doaj +1 more source