Results 101 to 110 of about 48,939 (293)

PARP inhibitors induce a senescence phenotype in non‐small cell lung carcinoma cell lines

FEBS Open Bio, EarlyView.
Talazoparib is the most potent inducer of senescence among different PARP1 inhibitors in human NSCLC cells. In the absence of PARP, no senescence phenotype was observed, demonstrating that PARP1 is necessary for the induction of senescence by this inhibitor.
Camille Huart, Manon Van den Abbeel, Christoph Schifflers, Karim Bouhjar, Andrea Scarmelotto, Alexis Khelfi, Anne‐Catherine Wera, Carine Michiels   +7 more
wiley   +1 more source

Fluorescence Lifetime Imaging Microscopy reveals rerouting of SNARE trafficking driving dendritic cell activation

eLife, 2017
SNARE proteins play a crucial role in intracellular trafficking by catalyzing membrane fusion, but assigning SNAREs to specific intracellular transport routes is challenging with current techniques.
Daniëlle Rianne José Verboogen, Natalia González Mancha, Martin ter Beest, Geert van den Bogaart   +3 more
doaj   +1 more source

Exo70-Mediated Recruitment of Nucleoporin Nup62 at the Leading Edge of Migrating Cells is Required for Cell Migration [PDF]

, 2009
Nucleoporin Nup62 localizes at the central channel of the nuclear pore complex and is essential for nucleocytoplasmic transport. Through its FG-repeat domain, Nup62 regulates nuclear pore permeability and binds nuclear transport receptors.
Bayliss, Bayliss, Bernad, Blower, Boyd, Bradford, Brown, Buss, Caswell, Cho, Clarkson, Dawlaty, De Corte, Dultz, Frey, Grindstaff, Gromley, He, Hsu, Inoue, Jeganathan, Joseph, Joseph, Kalverda, Kasper, Kawase, Kawato, Kirsh, Lee, Liu, Loiodice, Macaulay, Matunis, Melcak, Moore, Munson, Nagai, Orjalo, Oztan, Palancade, Palancade, Paschal, Pommereit, Prigent, Rabut, Rittmeyer, Rosse, Sakurai-Yageta, Schrader, Schwarz-Herion, Stewart, Taddei, Tayeb, Terry, Towbin, Van Impe, Wang, Ward, Yeaman, Zimmermann, Zuccolo, Zuo   +61 more
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Synergistic actions of v-SNARE transmembrane domains and membrane-curvature modifying lipids in neurotransmitter release

eLife, 2020
Vesicle fusion is mediated by assembly of SNARE proteins between opposing membranes. While previous work suggested an active role of SNARE transmembrane domains (TMDs) in promoting membrane merger (Dhara et al., 2016), the underlying mechanism remained ...
Madhurima Dhara, Maria Mantero Martinez, Mazen Makke, Yvonne Schwarz, Ralf Mohrmann, Dieter Bruns   +5 more
doaj   +1 more source

Genetic screen in Drosophila muscle identifies autophagy-mediated T-tubule remodeling and a Rab2 role in autophagy. [PDF]

, 2017
Transverse (T)-tubules make-up a specialized network of tubulated muscle cell membranes involved in excitation-contraction coupling for power of contraction. Little is known about how T-tubules maintain highly organized structures and contacts throughout
Fujita, Naonobu, Fukuda, Mitsunori, Groulx, Jean-Francois, Huang, Wilson, Jean, Steve, Kiger, Amy A, Koyama-Honda, Ikuko, Kuchitsu, Yoshihiko, Lin, Tzu-Han, Mizushima, Noboru, Nguyen, Jen   +10 more
core   +2 more sources

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

TRIP13 is a protein-remodeling AAA+ ATPase that catalyzes MAD2 conformation switching. [PDF]

, 2015
The AAA+ family ATPase TRIP13 is a key regulator of meiotic recombination and the spindle assembly checkpoint, acting on signaling proteins of the conserved HORMA domain family.
Corbett, Kevin D, Moeller, Arne, Rosenberg, Scott C, Speir, Jeffrey A, Su, Tiffany Y, Ye, Qiaozhen   +5 more
core   +2 more sources

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Sec3 promotes the initial binary t-SNARE complex assembly and membrane fusion

Nature Communications, 2017
Membrane fusion during exocytosis is mediated by interaction between SNARE proteins on vesicles and the cell membrane, but how SNARE complex assembly is initiated remains unknown.
Peng Yue, Yubo Zhang, Kunrong Mei, Shaoxiao Wang, Johannes Lesigang, Yueyao Zhu, Gang Dong, Wei Guo   +7 more
doaj   +1 more source