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Hemocyte Nuclei Isolation from Adult Drosophila melanogaster for snRNA-seq
Methods in Molecular Biology, 2023In adult Drosophila, most of the hemocytes are macrophage-like cells (so called plasmatocytes), which serve various functions in organ homeostasis and immune defense. Ontogeny and functions are largely conserved between vertebrate and invertebrate macrophages.
Fabian Hersperger +2 more
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snRNA-seq reveals a subpopulation of adipocytes that regulates thermogenesis
Nature, 2020Adipose tissue is usually classified on the basis of its function as white, brown or beige (brite)1. It is an important regulator of systemic metabolism, as shown by the fact that dysfunctional adipose tissue in obesity leads to a variety of secondary metabolic complications2,3.
Wenfei Sun, Hua Dong, Miroslav Balaz
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snRNA-seq of mouse brain cortex
2023GDF11 loxp mice aged 4-5M were treated in the prefrontal lobe with a focal injection of AAV9 CaMKIIa-Cre-P2A-GFP virus (KO group) or AAV9 CaMKIIa-GFP virus (Ctrl group). We used single nucleus RNA sequencing (snRNA-seq) to analyze the differential expressed genes (DEGs).
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snRNA-seq of Cervical Squamous Cell Carcinoma
2022To understand the etiological, structural, and immunological characteristics of cervical squamous cell carcinoma (CSCC), we conducted single nucleus RNA sequencing (snRNA-seq) experiments for cervical samples from 5 ...
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Glial changes and gene expression in Alzheimer's disease from snRNA-Seq and spatial transcriptomics
Journal of Alzheimer’s DiseaseBackground Alzheimer's disease (AD) is characterized by cortical atrophy, glutamatergic neuron loss, and cognitive decline. However, large-scale quantitative assessments of cellular changes during AD pathology remain scarce.
Songren Wei +9 more
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snRNA-seq of the cochlear nucleus
The molecular mechanisms underlying the complex functions of the cochlear nucleus (CN) remain poorly understood, requiring a thorough characterization of cellular properties. We utilized single-nuclei RNA sequencing (snRNA-seq) and spatial enhanced resolution omics-sequencing (Stereo-seq) to generate a comprehensive celluar and molecular atlas of the ...openaire +1 more source
Integrated snRNA-seq AnnData of paired primary–recurrent glioblastoma
We generated a processed AnnData object containing integrated and annotated single-nucleus RNA-seq profiles from paired primary and recurrent glioblastoma samples obtained from a publicly available GEO dataset (accession code GSE131928).openaire +1 more source