Results 61 to 70 of about 17,437 (131)

Single cell transcriptomes and multiscale networks from persons with and without Alzheimer’s disease

Nature Communications
The emergence of single nucleus RNA sequencing (snRNA-seq) offers to revolutionize the study of Alzheimer’s disease (AD). Integration with complementary multiomics data such as genetics, proteomics and clinical data provides powerful opportunities to ...
Qi Wang, Jerry Antone, Eric Alsop, Rebecca Reiman, Cory Funk, Jaroslav Bendl, Joel T. Dudley, Winnie S. Liang, Timothy L. Karr, Panos Roussos, David A. Bennett, Philip L. De Jager, Geidy E. Serrano, Thomas G. Beach, Kendall Van Keuren-Jensen, Diego Mastroeni, Eric M. Reiman, Benjamin P. Readhead   +17 more
doaj   +1 more source

Robust single-nucleus RNA sequencing reveals depot-specific cell population dynamics in adipose tissue remodeling during obesity

eLife
Single-nucleus RNA sequencing (snRNA-seq), an alternative to single-cell RNA sequencing (scRNA-seq), encounters technical challenges in obtaining high-quality nuclei and RNA, persistently hindering its applications.
Jisun So, Olivia Strobel, Jamie Wann, Kyungchan Kim, Avishek Paul, Dominic J Acri, Luke C Dabin, Jungsu Kim, Gang Peng, Hyun Cheol Roh   +9 more
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An optimized protocol for single nuclei isolation from clinical biopsies for RNA-seq

Scientific Reports, 2022
Single nuclei RNA sequencing (snRNA-seq) has evolved as a powerful tool to study complex human diseases. Single cell resolution enables the study of novel cell types, biological processes, cell trajectories, and cell–cell signaling pathways.
Thomas V. Rousselle, Jennifer M. McDaniels, Amol C. Shetty, Elissa Bardhi, Daniel G. Maluf, Valeria R. Mas   +5 more
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Systematic evaluation of single-cell multimodal data integration enhances cell type resolution and discovery of clinically relevant states in complex tissues

Genome Biology
Background The integration of multimodal single-cell data enables comprehensive organ reference atlases, yet its impact remains largely unexplored, particularly in complex tissues.
Mario Acera-Mateos, Xian Adiconis, Jessica-Kanglin Li, Domenica Marchese, Ginevra Caratù, Chung-Chau Hon, Prabha Tiwari, Miki Kojima, Beate Vieth, Michael A. Murphy, Sean K. Simmons, Thomas Lefevre, Irene Claes, Christopher L. O’Connor, Rajasree Menon, Edgar A. Otto, Yoshinari Ando, Katy Vandereyken, Matthias Kretzler, Markus Bitzer, Ernest Fraenkel, Thierry Voet, Wolfgang Enard, Piero Carninci, Holger Heyn, Joshua Z. Levin, Elisabetta Mereu   +26 more
doaj   +1 more source

Spatio-molecular gene expression reflects dorsal anterior cingulate cortex structure and function in the human brain

Cell Reports
Summary: The human brain’s dorsal anterior cingulate cortex (dACC) is critical in cognitive control, specifically reward processing and conflict monitoring, and regulates fear and pain expression.
Kinnary Shah, Michael S. Totty, Svitlana V. Bach, Madeline R. Valentine, Atharv Chandra, Haya A. AlGrain, Heena R. Divecha, Ryan A. Miller, Felix Rene M. Siewe, Sang Ho Kwon, Ishbel Del Rosario Alvia, Anthony D. Ramnauth, Madhavi Tippani, Sanjana Tyagi, Joel E. Kleinman, Leonardo Collado-Torres, Shizhong Han, Thomas M. Hyde, Stephanie C. Page, Kristen R. Maynard, Stephanie C. Hicks, Keri Martinowich   +21 more
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High‐Throughput Single‐Nucleus RNA Profiling of Minimal Puncture FFPE Samples Reveals Spatiotemporal Heterogeneity of Cancer

Advanced Science
Puncture biopsy, especially those preserved by formalin fixed paraffin embedding (FFPE) samples, play an important role in various research purposes.
Weiqin Jiang, Xiang Zhang, Ziye Xu, Qing Cheng, Xiaohan Li, Yuyi Zhu, Fangru Lu, Ling Dong, Linghui Zeng, Weixiang Zhong, Yongcheng Wang, Longjiang Fan, Hongyu Chen   +12 more
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Multiplexed snRNA-seq from frozen human brain samples v1

, 2020

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Sample Preparation of Human Eye Anterior Segment for sc/snRNA-seq v1

, 2022
This protocol is used for sample preparation of human donor eye for single cell/single nuclei RNA sequencing. The protocol consists of dissection, fresh and frozen tissue dissociation. The dissection of both anterior segment and posterior segment are included, but only anterior segment dissociation is described in this protocol.
openaire   +1 more source

Single-nucleus RNA sequencing dataset of diverse tissues from wild-type monkey and Tau-P301L transgenic monkey

Scientific Data
Utilizing non-human primates to study the role of human Tau and its related pathologies is logical and important due to their closer similarity to human brain structure and function.
Bofeng Han, Yan Chen, Weijie Ouyang, Danyi Chen, Jiawei Li, Weien Liang, Xudong Zhang, Chengxi Wei, Ling Liu, Sen Yan, Zhuchi Tu   +10 more
doaj   +1 more source

A single-cell and spatial RNA-seq database for Alzheimer’s disease (ssREAD)

Nature Communications
Alzheimer’s Disease (AD) pathology has been increasingly explored through single-cell and single-nucleus RNA-sequencing (scRNA-seq & snRNA-seq) and spatial transcriptomics (ST). However, the surge in data demands a comprehensive, user-friendly repository.
Cankun Wang, Diana Acosta, Megan McNutt, Jiang Bian, Anjun Ma, Hongjun Fu, Qin Ma   +6 more
doaj   +1 more source