Results 71 to 80 of about 15,972 (195)

A Viscous DES‐AAV‐Foxo1 Delivery System With High Transfection Efficiency for the Treatment of Corneal Endothelial Dysfunction by Restoring Mitochondria‐ER Contacts

Advanced Science, EarlyView.
High glucose triggers corneal endothelial dysfunction by impairing FOXO1‐mediated ITPR1 transcription, leading to disrupted mitochondria‐associated membrane (MAM) integrity and defective ER‐to‐mitochondria Ca2+ transfer. This study develops an innovative viscous DES‐AAV‐Foxo1 delivery system with enhanced transfection efficiency. This non‐invasive gene
Hongran Zhao, Xiaoyu Li, Hongwei Wang, Zongyi Li, Qun Wang, Yangyang Zhang, Xu Jing, Xia Qi, Qingjun Zhou, Shengqian Dou, Lixin Xie   +10 more
wiley   +1 more source

Dissecting the molecular landscape of Parkinson’s disease and Parkinson’s disease dementia using highly efficient snRNA-seq (HIF-snRNA-seq)

Abstract This study presents a transcriptomic analysis of the cingulate cortex (CING) in Parkinson’s disease (PD) and Parkinson’s disease dementia (PDD) using a High-efficiency single-nucleus RNA sequencing (HiF-snRNA-seq) protocol optimized for post-mortem brain samples.
Sung-Ung Kang, Jinhee Park, Shinwon Ha, Dongsan Kim, Olga Pletnikova, Javier Redding-Ochoa, Juan C. Troncoso, Quan Peng, Beth O. Van Emburgh, Jaldhir Trivedi, Saurav Brahmachari, Bardia Nezami, Valina L. Dawson, Ted M. Dawson   +13 more
openaire   +2 more sources

Combining Spatial Multi‐Omics Data to Decipher Spatial Domains and Elucidate Cell Heterogeneity Based on Self‐Supervised Graph Learning

Advanced Science, EarlyView.
A self‐supervised multi‐view graph fusion framework integrates spatial multi‐omics, excelling in domain identification and denoising. It reconstructs spatial pseudo‐expression, jointly analyzes multi‐omics data, infers RNA velocity, predicts spatial omics features from single‐cell multi‐omics, and detects spatially dark genes and transcription factors,
Yuejing Lu, Rui Qiao, Ying Li, Junhong Li, Nuoya Yue, Jing Ge, Jiao Wang, Luonan Chen, Peiluan Li   +8 more
wiley   +1 more source

Xenograft snRNA-seq Integration and Normalization v1

This protocol describes the integration and normalization of single-nucleus RNA sequencing (snRNA-seq) data obtained from xenograft samples. The process involves loading data from multiple Cell Ranger outputs, creating Seurat objects, performing data integration, and normalizing the datasets.
Zac Chatterton, Chiara Pavan, Clare Parish   +2 more
openaire   +1 more source

“Membrane‐Guided” Repair Strategy: Precision Delivery of GGT1 Degrader for Targeted Repair and Regeneration of Spinal Cord Neurons

Advanced Science, EarlyView.
This study confirms that GGT1 is a key driver of neuronal ferroptosis following spinal cord injury. We developed NSCm@EA, a biomimetic delivery system coated with neural stem cell membranes, for precise delivery of enocyanin to injured neurons. By combining targeted delivery with ubiquitination degradation mechanisms, this system promotes MGRN1 ...
Tao Yang, Lei Ye, Peigen Xie, Tao Song, Jian Chen, Heng Zhao, Zhengshan Liu, Xi Chen, Jianing Ding, Xintian Ding, Ao Shao, Miaomiao Wu, Fengdong Zhao, Siyue Tao, Tao You   +14 more
wiley   +1 more source

Lactoferrin Deficiency During Lactation Causes Adult Obesity‐Related Metabolic Disease Through Persistent Adipose Dysfunction Driven by Impaired Adipocyte Development

Advanced Science, EarlyView.
Lactational lactoferrin deficiency exerts lasting effects on epididymal adipose tissue development from lactation into adulthood: it impairs adipocyte hyperplasia and induces pathological hypertrophy, resulting in lower body weight yet exacerbated metabolic dysfunction under a high‐fat diet in adulthood.
Qin An, Yunxia Zou, Wenli Wang, Zhimei Cheng, Zhuoxing Zhang, Ruwei Liu, Xiong Wang, Kunlun Huang, Fangrong Ding, Yunping Dai, Qingyong Meng, Yali Zhang   +11 more
wiley   +1 more source

Systematic evaluation of single-cell multimodal data integration enhances cell type resolution and discovery of clinically relevant states in complex tissues

Genome Biology
Background The integration of multimodal single-cell data enables comprehensive organ reference atlases, yet its impact remains largely unexplored, particularly in complex tissues.
Mario Acera-Mateos, Xian Adiconis, Jessica-Kanglin Li, Domenica Marchese, Ginevra Caratù, Chung-Chau Hon, Prabha Tiwari, Miki Kojima, Beate Vieth, Michael A. Murphy, Sean K. Simmons, Thomas Lefevre, Irene Claes, Christopher L. O’Connor, Rajasree Menon, Edgar A. Otto, Yoshinari Ando, Katy Vandereyken, Matthias Kretzler, Markus Bitzer, Ernest Fraenkel, Thierry Voet, Wolfgang Enard, Piero Carninci, Holger Heyn, Joshua Z. Levin, Elisabetta Mereu   +26 more
doaj   +1 more source

Spatio-molecular gene expression reflects dorsal anterior cingulate cortex structure and function in the human brain

Cell Reports
Summary: The human brain’s dorsal anterior cingulate cortex (dACC) is critical in cognitive control, specifically reward processing and conflict monitoring, and regulates fear and pain expression.
Kinnary Shah, Michael S. Totty, Svitlana V. Bach, Madeline R. Valentine, Atharv Chandra, Haya A. AlGrain, Heena R. Divecha, Ryan A. Miller, Felix Rene M. Siewe, Sang Ho Kwon, Ishbel Del Rosario Alvia, Anthony D. Ramnauth, Madhavi Tippani, Sanjana Tyagi, Joel E. Kleinman, Leonardo Collado-Torres, Shizhong Han, Thomas M. Hyde, Stephanie C. Page, Kristen R. Maynard, Stephanie C. Hicks, Keri Martinowich   +21 more
doaj   +1 more source

Single‐Cell Annotation and Localization via Integrating Spatial Transcriptomics Maps the Mouse Ocular Atlas and RAO Dynamics

Advanced Science, EarlyView.
We developed the ASCAL pipeline, integrating complementary spatial transcriptomics, to construct a high‐fidelity mouse whole‐eye single‐cell atlas. Applying ASCAL to a retinal artery occlusion (RAO) model revealed spatially restricted immune activation localized to the ganglion cell layer and the selective depletion of a translationally active, outer ...
Chen Du, Yinming Li, Ziyue Li, Yuedan Wang, Shaopeng Li, Yijie Wang, Yingying Qu, Bingyang Lv, Ying Li, Ting Chen, Yu Zhou, Xuan Xiao   +11 more
wiley   +1 more source

High‐Throughput Single‐Nucleus RNA Profiling of Minimal Puncture FFPE Samples Reveals Spatiotemporal Heterogeneity of Cancer

Advanced Science
Puncture biopsy, especially those preserved by formalin fixed paraffin embedding (FFPE) samples, play an important role in various research purposes.
Weiqin Jiang, Xiang Zhang, Ziye Xu, Qing Cheng, Xiaohan Li, Yuyi Zhu, Fangru Lu, Ling Dong, Linghui Zeng, Weixiang Zhong, Yongcheng Wang, Longjiang Fan, Hongyu Chen   +12 more
doaj   +1 more source