Results 71 to 80 of about 17,437 (131)

RNAlater-compatible protocol for nuclei isolation from radical prostatectomy prostate cancer resections, enabling single-nucleus resolution RNA-seq

Scientific Reports
We present an optimised protocol for single-nucleus isolation from RNAlater-preserved prostate cancer (PC) resections that enables transcriptomically robust snRNA-seq.
Magdalena Julita Krystkiewicz-Orzechowska, Dorota Anusewicz, Katarzyna Kośla, Izabela Baryła, Agata Sakowicz, Michał Olczak, Mateusz Mroczek, Marek Lipiński, Andrzej K. Bednarek   +8 more
doaj   +1 more source

Optimized Method for High-Quality Isolation of Single-Nuclei From Mosquito Fat Body for RNA Sequencing

Bio-Protocol
Single-cell and single-nucleus RNA sequencing are revolutionizing our understanding of cellular biology. The identification of molecular markers, single-cell transcriptomic profiling, and differential gene expression at the cellular level has revealed ...
Stephanie de Carvalho, Colton McNinch, Carolina Barillas-Mury   +2 more
doaj   +1 more source

Collection and shipment of specimen for single-nuclei RNA sequencing (snRNA-Seq) v1

These samples are part of the JAX-Sen project in the SenNet Consortium. Here we provide details on specimen collection and shipment to the Robson laboratory at The Jackson Laboratory for Genomic Medicine (JAX-GM) in Farmington, CT for its processing for single-nuclei RNA-sequencing (snRNA-seq).
Laura Robinson, Susan Sheehan, Gaven Garland, Ron Korstanje   +3 more
openaire   +1 more source

Identification of epileptic hippocampal sclerosis related genes through bulk and single-nucleus RNA sequencing datasets

Cellular and Molecular Life Sciences
Background Hippocampal sclerosis is one of the most common pathological alterations in drug-refractory epilepsy. However, the exact causes and molecular mechanisms of its formation remain unknown. Methods We constructed a hippocampal sclerosis score (HSS)
Jincheng Wang, Zhongyu Zhou, Die Wu, Yiqiao Zeng, Hanyu Huang, Qigang Zhou, Fan Meng   +6 more
doaj   +1 more source

Single-nucleus RNA sequencing reveals transcriptional response of wheat roots and leaves to salt stress

Crop Journal
Deeper understanding of the mechanism by which wheat responds to salt stress (SS) remains a major challenge due to the scarcity of available single-cell/nucleus transcriptomics resources.
Linyi Qiao, Hong Liu, Tian Li, Yiming Ma, Jingjing Ning, Zhijian Chang, Junming Li, Xigang Liu, Zhiyong Zhao, Xingwei Zheng, Jiajia Zhao, Jun Zheng   +11 more
doaj   +1 more source

An improved nuclei isolation protocol from leaf tissue for single-cell transcriptomics.

PLoS ONE
The study of plant biology has traditionally focused on investigations conducted at the tissue, organ, or whole plant level. However, single-cell transcriptomics has recently emerged as an important tool for plant biology, enabling researchers to uncover
Gabriela Madrid, Gabriel Angelo Saraiva Raimundo, Fabian Andres Reyes, Edgard Augusto de Toledo Picoli, Marcio F R Resende, Kelly M Balmant   +5 more
doaj   +1 more source

Whole organism snRNA-seq reveals systemic peripheral changes in Alzheimer’s Disease fly models

Abstract Peripheral tissues become disrupted in Alzheimer’s Disease (AD). However, a comprehensive understanding of how the expression of AD-associated toxic proteins, Aβ42 and Tau, in neurons impacts the periphery is lacking.
Ye-Jin Park, Tzu-Chiao Lu, Tyler Jackson, Lindsey D. Goodman, Lindsey Ran, Jiaye Chen, Chung-Yi Liang, Erin Harrison, Christina Ko, Ao-Lin Hsu, Shinya Yamamoto, Yanyan Qi, Hugo J. Bellen, Hongjie Li   +13 more
openaire   +2 more sources

The snRNA-seq data analysis of hippocampal interneurons resolves 15 distinct lineages

Erythropoietin (EPO) aids in rectifying hippocampal transcriptional networks and synaptic structures of pyramidal lineages, thereby mitigating mood and cognition-associated disorders. An imminent conundrum is how EPO restores synapses by involving interneurons.
openaire   +2 more sources

DECIPHERING THE INTERNEURON DIVERSITY IN THE HUMAN CAUDATE NUCLEUS AND PUTAMEN USING SNRNA-SEQ.

IBRO Neuroscience Reports, 2023
Ana Muñoz Manchado, Leonardo Garma, Lisbeth Harder, Juan Manuel Barba Reyes, Mónica Díez Salguero, Alberto Serrano Pozo, Bradley Hyman   +6 more
openaire   +2 more sources

Hippocampal snRNA-seq in Collaborative Cross reveals molecular signatures of cognitive resilience independent of chronological aging. [PDF]

Alzheimers Dement
Chen Y, Ouellette AR, Cao Y, Zhang J, Kaczorowski CC.   +4 more
europepmc   +1 more source