Results 51 to 60 of about 301,220 (263)

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

Lipopolysaccharide uptake is augmented in lipopolysaccharide‐tolerant mouse macrophage‐like cells via increased CD14 expression

FEBS Open Bio, EarlyView.
In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara, Takayuki Manabe, Mika Jouta, Kiyoshi Kawasaki   +3 more
wiley   +1 more source

Determination of the thermodynamic parameters and critical micelle concentration in the water-toluene-sodium dodecyl sulfate emulsion system in the presence of methyl methacrylate [PDF]

شیمی کاربردی روز, 2014
In this study, the effect of methyl methacrylate on the stability of the emulsion system and its distribution in water- toluene system, half decay time, surface tension by drip method and critical micelle concentration by conductometry method were ...
Fariborz Azizinezhad, Salimeh Rezayi, Mohsen Shabani   +2 more
doaj   +1 more source

Reversible Denaturation of Enzymes by Sodium Dodecyl Sulfate

Journal of Biological Chemistry, 1971
Successful renaturation of several oligomeric enzymes after denaturation by sodium dodecyl sulfate is described. The results obtained suggest a general procedure for reactivation of enzymes after treatment with this detergent. Proteins in dodecyl sulfate solution are first incubated in concentrated urea and are then freed of the detergent by an anion ...
K, Weber, D J, Kuter
openaire   +2 more sources

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Retention behavior of selected alkaloids in Reversed Phase micellar chromatographic systems

Current Issues in Pharmacy and Medical Sciences, 2015
In this work, the effects of sodium dodecyl sulfate (SDS) concentrations on retention, separation selectivity, peak shapes and systems efficiency were investigated. Herein, the retention data for 11 alkaloids were determined on an RP18 silica column with
Petruczynik Anna, Noster Grzegorz, Waksmundzka-Hajnos Monika   +2 more
doaj   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

FEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

Chitosan/sodium dodecyl sulfate microcapsules preparation: Influence of applied method [PDF]

Journal of the Serbian Chemical Society
This study aims to compare and analyse the synthesis of microcapsules stabilized with chitosan/sodium dodecyl sulphate complex according to emulsion preparation methods.
Milinković Budinčić Jelena, Petrović Lidija, Aleksić Milijana, Fraj Jadranka, Đekić Ljiljana, Bučko Sandra, Katona Jaroslav, Spasojević Ljiljana, Ostojić Jelena, Radonić Željana, Bosnić Mina   +10 more
doaj   +1 more source

Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region

FEBS Open Bio, EarlyView.
Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson, Anja H. Schiemann, Evelyn Sattlegger   +2 more
wiley   +1 more source

Exploring the nature of the interactions between the molecules of the sodium dodecyl sulfate and water in crystal phases and in the water/vacuum interface

Heliyon, 2020
The nature of the interaction between the molecules of the sodium dodecyl sulfate surfactant forming two crystal phases, one anhydrous, NaC12H25O4S and the other, NaC12H25O4S.H2O, hydrated with one water molecule for unit cell, has been studied in detail
Yosslen Aray, José G. Parra, Ricardo Paredes, Luis Javier Álvarez, Antonio Diaz-Barrios   +4 more
doaj   +1 more source